##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI AspI AviII BbrPI
BciVI BfrI BlpI Bpu1102I BsiWI
Bsp1407I BspDI BspHI BsrGI BstBI
CelII ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRV
FseI FspI HindIII HpaI MluI
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SbfI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 105 GACGTC GACGT^C
Acc65I 1002 GGTACC G^GTACC
AccIII 2120 TCCGGA T^CCGGA
AhdI 2404 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1941 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1002 GGTACC G^GTACC
AspEI 2404 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2931 CCTAGG C^CTAGG
BbeI 897 GGCGCC GGCGC^C
BclI 2865 TGATCA T^GATCA
BglII 2663 AGATCT A^GATCT
BlnI 2931 CCTAGG C^CTAGG
BsaBI 348 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2120 TCCGGA T^CCGGA
BsiEI 1139 CGACCG CG[GA][CT]^CG
BspEI 2120 TCCGGA T^CCGGA
BssSI 1873 CACGAG CACGAG(-5/-1)
Bst1107I 526 GTATAC GTA^TAC
BstAPI 2060 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 526 GTATAC GTA^TAC
DraI 3356 TTTAAA TTT^AAA
Eam1105I 2404 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2245 GAATTC G^AATTC
EcoT22I 3293 ATGCAT ATGCA^T
FbaI 2865 TGATCA T^GATCA
KasI 897 GGCGCC G^GCGCC
KpnI 1002 GGTACC GGTAC^C
MamI 348 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2849 CAATTG C^AATTG
MroI 2120 TCCGGA T^CCGGA
MunI 2849 CAATTG C^AATTG
NarI 897 GGCGCC GG^CGCC
NdeI 1613 CATATG CA^TATG
NsiI 3293 ATGCAT ATGCA^T
PflMI 2994 CCACCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 583 GAGCTC GAGCT^C
SalI 919 GTCGAC G^TCGAC
SapI 2970 GCTCTTC GCTCTTC(1/4)
ScaI 3611 AGTACT AGT^ACT
SexAI 2198 ACCAGGT A^CC[AT]GGT
SfoI 897 GGCGCC GGC^GCC
SgrAI 831 CGCCGGCG C[GA]^CCGG[CT]G
StuI 2135 AGGCCT AGG^CCT
Van91I 2994 CCACCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1941 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 819 GTGCAC G^TGCAC
826 GTGCAC
ApaLI 819 GTGCAC G^TGCAC
826 GTGCAC
BcgI 2390 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1997 GCACGGTGATCG
BsaAI 315 CACGTA [CT]AC^GT[GA]
2806 TACGTG
BsmBI 2034 CGTCTC CGTCTC(1/5)
1662 GAGACG
BsmI 617 GCATTC GAATGC(1/-1)
1419 GCATTC
BspLU11I 2448 ACATGT A^CATGT
3812 ACATGT
BsrDI 3501 CATTGC GCAATG(2/0)
3048 GCAATG
BstEII 870 GGTGACC G^GT[ACGT]ACC
1455 GGTCACC
BstPI 870 GGTGACC G^GT[ACGT]ACC
1455 GGTCACC
EcoO65I 870 GGTGACC G^GT[ACGT]ACC
1455 GGTCACC
HincII 919 GTCGAC GT[CT]^[GA]AC
2437 GTCAAC
HindII 919 GTCGAC GT[CT]^[GA]AC
2437 GTCAAC
NcoI 2189 CCATGG C^CATGG
2633 CCATGG
NspI 2448 ACATGT [GA]CATG^[CT]
3812 ACATGT
PaeR7I 186 CTCGAG C^TCGAG
3239 CTCGAG
PshAI 1104 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3914 GACGGTTGTC
XhoI 186 CTCGAG C^TCGAG
3239 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI