##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp700I Asp718I BamHI BbrPI
BcgI BciVI BclI BfrI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BstBI ClaI DraIII DrdI
EcoRI EcoRV EcoT22I FbaI HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI ScaI SfuI SnaBI
SpeI SspBI SspI Tsp509I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 1297 ACGTGT A^C[GA][CT]GT
Alw44I 1869 GTGCAC G^TGCAC
AlwI 30 GGATC GGATC(4/5)
Aor51HI 245 AGCGCT AGC^GCT
ApaLI 1869 GTGCAC G^TGCAC
AviII 2641 TGCGCA TGC^GCA
AvrII 2807 CCTAGG C^CTAGG
BbeI 367 GGCGCC GGCGC^C
BbsI 2246 GAAGAC GAAGAC(2/6)
BfaI 2808 CTAG C^TAG
BlnI 2807 CCTAGG C^CTAGG
BpuAI 2246 GAAGAC GAAGAC(2/6)
BspHI 304 TCATGA T^CATGA
BsrBI 69 GAGCGG CCGCTC(-3/-3)
Bst1107I 885 GTATAC GTA^TAC
BstZ17I 885 GTATAC GTA^TAC
CpoI 2434 CGGACCG CG^G[AT]CCG
Eco47III 245 AGCGCT AGC^GCT
EcoNI 707 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 3036 GGCCGGCC GGCCGG^CC
FspI 2641 TGCGCA TGC^GCA
HincII 1431 GTCGAC GT[CT]^[GA]AC
HindII 1431 GTCGAC GT[CT]^[GA]AC
HindIII 1836 AAGCTT A^AGCTT
KasI 367 GGCGCC G^GCGCC
KspI 2740 CCGCGG CCGC^GG
MaeI 2808 CTAG C^TAG
NarI 367 GGCGCC GG^CGCC
RcaI 304 TCATGA T^CATGA
RsrII 2434 CGGACCG CG^G[AT]CCG
SacI 279 GAGCTC GAGCT^C
SacII 2740 CCGCGG CCGC^GG
SalI 1431 GTCGAC G^TCGAC
SfiI 41 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 367 GGCGCC GGC^GCC
SgrAI 1667 CGCCGGTG C[GA]^CCGG[CT]G
StuI 593 AGGCCT AGG^CCT
SwaI 3283 ATTTAAAT ATTT^AAAT
TfiI 2970 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 2607 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3269 GACTAAGAGTC
AspEI 2607 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3269 GACTAAGAGTC
AspHI 279 GAGCTC G[AT]GC[AT]^C
1869 GTGCAC
AspI 552 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2211 GACTACGTC
BbvCI 471 CCTCAGC CCTCAGC(-5/-2)
2576 CCTCAGC
BglII 400 AGATCT A^GATCT
2545 AGATCT
BsaI 1057 GAGACC GGTCTC(1/5)
1191 GAGACC
BsiEI 15 CGGCCG CG[GA][CT]^CG
1288 CGGCCG
BsiHKAI 279 GAGCTC G[AT]GC[AT]^C
1869 GTGCAC
BsrDI 1798 GCAATG GCAATG(2/0)
2014 GCAATG
BstAPI 1721 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2441 GCAGCTGCTGC
BstYI 400 AGATCT [GA]^GATC[CT]
2545 AGATCT
DraI 3284 TTTAAA TTT^AAA
3297 TTTAAA
EagI 15 CGGCCG C^GGCCG
1288 CGGCCG
Eam1105I 2607 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3269 GACTAAGAGTC
EarI 2028 CTCTTC CTCTTC(1/4)
2451 CTCTTC
EclXI 15 CGGCCG C^GGCCG
1288 CGGCCG
Eco52I 15 CGGCCG C^GGCCG
1288 CGGCCG
Ksp632I 2028 CTCTTC CTCTTC(1/4)
2451 CTCTTC
MflI 400 AGATCT [GA]^GATC[CT]
2545 AGATCT
MseI 3285 TTAA T^TAA
3298 TTAA
NcoI 808 CCATGG C^CATGG
1030 CCATGG
PflFI 552 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2211 GACTACGTC
SapI 2027 GCTCTTC GCTCTTC(1/4)
2450 GCTCTTC
SbfI 1727 CCTGCAGG CCTGCA^GG
3118 CCTGCAGG
SmlI 396 CTCAAG C^T[CT][GA]AG
1935 CTTGAG
Sse8387I 1727 CCTGCAGG CCTGCA^GG
3118 CCTGCAGG
Tru9I 3285 TTAA T^TAA
3298 TTAA
Tth111I 552 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2211 GACTACGTC
XcmI 467 CCAGCCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2542 CCAAGATCTTTGTGG
XhoII 400 AGATCT [GA]^GATC[CT]
2545 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI AocI ApaI AvaI
AvaII BalI BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SphI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XmaCI
XmaI