##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BcgI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FauI FbaI
FseI FspI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SpeI Sse8387I StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1697 GGTACC G^GTACC
AccI 2131 GTCTAC GT^[AC][GT]AC
AcyI 383 GGCGCC G[GA]^CG[CT]C
AflII 1875 CTTAAG C^TTAAG
ApaI 1118 GGGCCC GGGCC^C
Asp718I 1697 GGTACC G^GTACC
BalI 906 TGGCCA TGG^CCA
BamHI 2613 GGATCC G^GATCC
BbeI 383 GGCGCC GGCGC^C
BbrPI 705 CACGTG CAC^GTG
BbvCI 1236 GCTGAGG CCTCAGC(-5/-2)
BciVI 2453 GTATCC GTATCC(6/5)
BfrI 1875 CTTAAG C^TTAAG
BsaHI 383 GGCGCC G[GA]^CG[CT]C
BsrBI 2325 CCGCTC CCGCTC(-3/-3)
BsrFI 66 GCCGGC [GA]^CCGG[CT]
BstXI 2570 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 66 GCCGGC [GA]^CCGG[CT]
DraIII 182 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1983 GAAGAG CTCTTC(1/4)
EcoNI 607 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1703 GATATC GAT^ATC
HgaI 1826 GACGC GACGC(5/10)
Hin1I 383 GGCGCC G[GA]^CG[CT]C
HincII 2192 GTTAAC GT[CT]^[GA]AC
HindII 2192 GTTAAC GT[CT]^[GA]AC
HindIII 1551 AAGCTT A^AGCTT
HpaI 2192 GTTAAC GTT^AAC
KasI 383 GGCGCC G^GCGCC
KpnI 1697 GGTACC GGTAC^C
Ksp632I 1983 GAAGAG CTCTTC(1/4)
KspI 73 CCGCGG CCGC^GG
MluNI 906 TGGCCA TGG^CCA
MscI 906 TGGCCA TGG^CCA
NaeI 66 GCCGGC GCC^GGC
NarI 383 GGCGCC GG^CGCC
NgoMIV 66 GCCGGC G^CCGGC
PflMI 387 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 705 CACGTG CAC^GTG
PmlI 705 CACGTG CAC^GTG
PstI 1811 CTGCAG CTGCA^G
SacII 73 CCGCGG CCGC^GG
SapI 1983 GAAGAGC GCTCTTC(1/4)
SfoI 383 GGCGCC GGC^GCC
SnaBI 2502 TACGTA TAC^GTA
Van91I 387 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1097 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 74 CGCG CG^CG
2065 CGCG
AflIII 798 ACATGT A^C[GA][CT]GT
2162 ACATGT
AlwNI 278 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGTGGCTG
Asp700I 851 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1656 GAAAATGTTC
BbsI 2361 GAAGAC GAAGAC(2/6)
489 GTCTTC
BpuAI 2361 GAAGAC GAAGAC(2/6)
489 GTCTTC
BsaAI 705 CACGTG [CT]AC^GT[GA]
2502 TACGTA
BsmI 1225 GAATGC GAATGC(1/-1)
2223 GAATGC
BspHI 144 TCATGA T^CATGA
1668 TCATGA
BspLU11I 798 ACATGT A^CATGT
2162 ACATGT
BstAPI 181 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
983 GCAGTCTGTGC
BstUI 74 CGCG CG^CG
2065 CGCG
BstYI 200 AGATCC [GA]^GATC[CT]
2613 GGATCC
DraI 1528 TTTAAA TTT^AAA
1718 TTTAAA
DsaI 73 CCGCGG C^C[GA][CT]GG
2795 CCGTGG
EcoT14I 2684 CCAAGG C^C[AT][AT]GG
2761 CCTTGG
EcoT22I 750 ATGCAT ATGCA^T
1999 ATGCAT
MflI 200 AGATCC [GA]^GATC[CT]
2613 GGATCC
MspA1I 73 CCGCGG C[AC]G^C[GT]G
419 CCGCTG
MvnI 74 CGCG CG^CG
2065 CGCG
NsiI 750 ATGCAT ATGCA^T
1999 ATGCAT
RcaI 144 TCATGA T^CATGA
1668 TCATGA
SacI 950 GAGCTC GAGCT^C
1332 GAGCTC
SphI 1468 GCATGC GCATG^C
1880 GCATGC
StyI 2684 CCAAGG C^C[AT][AT]GG
2761 CCTTGG
XhoII 200 AGATCC [GA]^GATC[CT]
2613 GGATCC
XmnI 851 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1656 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BssSI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRI EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI