##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BpmI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AlwI 358 GGATC GGATC(4/5)
ApaI 1009 GGGCCC GGGCC^C
AseI 1554 ATTAAT AT^TAAT
AsnI 1554 ATTAAT AT^TAAT
AspHI 1305 GAGCAC G[AT]GC[AT]^C
AvaII 1240 GGTCC G^G[AT]CC
AviII 5 TGCGCA TGC^GCA
BbrPI 1312 CACGTG CAC^GTG
BcnI 508 CCCGG CC^[GC]GG
BglII 150 AGATCT A^GATCT
BlpI 937 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 937 GCTGAGC GC^T[ACGT]AGC
BseRI 631 GAGGAG GAGGAG(10/8)
BsiHKAI 1305 GAGCAC G[AT]GC[AT]^C
BspMI 969 ACCTGC ACCTGC(4/8)
BsrBI 27 GAGCGG CCGCTC(-3/-3)
CelII 937 GCTGAGC GC^T[ACGT]AGC
DraI 2028 TTTAAA TTT^AAA
DraIII 1141 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 698 CCATGG C^C[GA][CT]GG
EcoRI 1078 GAATTC G^AATTC
EcoT14I 698 CCATGG C^C[AT][AT]GG
FspI 5 TGCGCA TGC^GCA
HindIII 1043 AAGCTT A^AGCTT
MfeI 9 CAATTG C^AATTG
MunI 9 CAATTG C^AATTG
NciI 508 CCCGG CC^[GC]GG
NcoI 698 CCATGG C^CATGG
PleI 1093 GACTC GAGTC(4/5)
PmaCI 1312 CACGTG CAC^GTG
PmlI 1312 CACGTG CAC^GTG
PshAI 178 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1554 ATTAAT AT^TAAT
PstI 1813 CTGCAG CTGCA^G
SapI 1201 GAAGAGC GCTCTTC(1/4)
SnaBI 1568 TACGTA TAC^GTA
SpeI 2003 ACTAGT A^CTAGT
SspI 667 AATATT AAT^ATT
StyI 698 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 1410 CCGC CCGC(-3/-1)
29 GCGG
BbsI 847 GAAGAC GAAGAC(2/6)
529 GTCTTC
BpuAI 847 GAAGAC GAAGAC(2/6)
529 GTCTTC
BsaBI 145 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1648 GATTTAGATC
BsmFI 110 GGGAC GGGAC(10/14)
1118 GTCCC
BsrDI 534 CATTGC GCAATG(2/0)
1832 GCAATG
BstF5I 339 CATCC GGATG(2/0)
834 CATCC
BstYI 150 AGATCT [GA]^GATC[CT]
358 GGATCT
CfoI 6 GCGC GCG^C
436 GCGC
FokI 339 CATCC GGATG(9/13)
834 CATCC
HaeIII 1010 GGCC GG^CC
1180 GGCC
HapII 509 CCGG C^CGG
1097 CCGG
HhaI 6 GCGC GCG^C
436 GCGC
HinP1I 6 GCGC G^CGC
436 GCGC
HpaII 509 CCGG C^CGG
1097 CCGG
MamI 145 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1648 GATTTAGATC
MflI 150 AGATCT [GA]^GATC[CT]
358 GGATCT
MspI 509 CCGG C^CGG
1097 CCGG
NlaIV 702 GGTTCC GG[ACGT]^[ACGT]CC
1009 GGGCCC
XhoII 150 AGATCT [GA]^GATC[CT]
358 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanII
BbvI BfaI BmyI BsaAI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I