##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BlnI BsaBI
BsaHI BsaWI BseAI BsiWI BspDI
BspEI BssSI Bsu36I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SnaBI SpeI SphI Sse8387I
StuI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 1989 CTTAAG C^TTAAG
AhdI 1560 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 103 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 239 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1560 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2688 GTGCTC G[AT]GC[AT]^C
AspI 153 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2302 GGATCC G^GATCC
BanI 307 GGCACC G^G[CT][GA]CC
BbsI 1234 GAAGAC GAAGAC(2/6)
BfrI 1989 CTTAAG C^TTAAG
BpuAI 1234 GAAGAC GAAGAC(2/6)
BsaAI 2883 TACGTG [CT]AC^GT[GA]
BsaI 1366 GGTCTC GGTCTC(1/5)
BsiEI 369 CGACCG CG[GA][CT]^CG
BsiHKAI 2688 GTGCTC G[AT]GC[AT]^C
BsmBI 158 CGTCTC CGTCTC(1/5)
BspMI 2003 ACCTGC ACCTGC(4/8)
BsrFI 19 ACCGGC [GA]^CCGG[CT]
BssHII 403 GCGCGC G^CGCGC
BstAPI 673 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3208 GGTGACC G^GT[ACGT]ACC
BstPI 3208 GGTGACC G^GT[ACGT]ACC
BstXI 480 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 19 ACCGGC [GA]^CCGG[CT]
DraII 1542 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 311 CCATGG C^C[GA][CT]GG
Eam1105I 1560 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 378 CCTCCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1542 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3208 GGTGACC G^GT[ACGT]ACC
EcoT14I 311 CCATGG C^C[AT][AT]GG
HgaI 157 GCGTC GACGC(5/10)
MfeI 455 CAATTG C^AATTG
MunI 455 CAATTG C^AATTG
NcoI 311 CCATGG C^CATGG
NheI 639 GCTAGC G^CTAGC
PflFI 153 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1542 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1442 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1208 CAGCTG CAG^CTG
ScaI 1418 AGTACT AGT^ACT
SexAI 1004 ACCTGGT A^CC[AT]GGT
SgrAI 18 CACCGGCG C[GA]^CCGG[CT]G
StyI 311 CCATGG C^C[AT][AT]GG
Tth111I 153 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1876 TCTAGA T^CTAGA
XcmI 2093 CCACGTTAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 239 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
BglII 888 AGATCT A^GATCT
925 AGATCT
BlpI 84 GCTGAGC GC^T[ACGT]AGC
324 GCTGAGC
BpmI 644 CTGGAG CTGGAG(16/14)
683 CTGGAG
Bpu1102I 84 GCTGAGC GC^T[ACGT]AGC
324 GCTGAGC
BseRI 502 GAGGAG GAGGAG(10/8)
2391 GAGGAG
BsmFI 71 GGGAC GGGAC(10/14)
1491 GGGAC
BsmI 1037 GAATGC GAATGC(1/-1)
2219 GAATGC
BspLU11I 1282 ACATGT A^CATGT
1660 ACATGT
Bst1107I 1656 GTATAC GTA^TAC
1888 GTATAC
BstBI 217 TTCGAA TT^CGAA
955 TTCGAA
BstNI 108 CCTGG CC^[AT]GG
1005 CCTGG
BstZ17I 1656 GTATAC GTA^TAC
1888 GTATAC
CelII 84 GCTGAGC GC^T[ACGT]AGC
324 GCTGAGC
DraIII 989 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3114 CACATTGTG
EaeI 14 CGGCCA [CT]^GGCC[GA]
35 CGGCCA
EarI 499 GAAGAG CTCTTC(1/4)
1201 GAAGAG
EcoRII 108 CCTGG ^CC[AT]GG
1005 CCTGG
HaeII 81 GGCGCT [GA]GCGC^[CT]
328 AGCGCC
Ksp632I 499 GAAGAG CTCTTC(1/4)
1201 GAAGAG
MslI 2314 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3202 CATATTGGTG
MspA1I 32 CAGCGG C[AC]G^C[GT]G
1208 CAGCTG
MvaI 108 CCTGG CC^[AT]GG
1005 CCTGG
NdeI 1068 CATATG CA^TATG
1196 CATATG
NspI 1282 ACATGT [GA]CATG^[CT]
1660 ACATGT
NspV 217 TTCGAA TT^CGAA
955 TTCGAA
SfuI 217 TTCGAA TT^CGAA
955 TTCGAA
SmaI 68 CCCGGG CCC^GGG
608 CCCGGG
SspI 1850 AATATT AAT^ATT
2203 AATATT
SwaI 2484 ATTTAAAT ATTT^AAAT
3272 ATTTAAAT
XmaCI 68 CCCGGG C^CCGGG
608 CCCGGG
XmaI 68 CCCGGG C^CCGGG
608 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BsaJI BsgI
BsiYI BslI BsmAI BsoBI Bsp1286I
Bsp1407I BspHI BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI PleI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII