##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AclI AgeI AocI
Aor51HI AscI Asp718I AvrII BanI
BbeI BciVI BglII BlnI BsaBI
BsiWI BsmI BspDI BsrBI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraIII DrdI
Eco47III Eco81I EcoO65I EcoT22I KasI
KpnI MamI MluI NarI NdeI
NheI NotI NruI NsiI PacI
PflMI PinAI PmeI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SfoI SgrAI SnaBI SpeI Sse8387I
StuI Van91I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2068 GACGTC GACGT^C
AccIII 3674 TCCGGA T^CCGGA
Alw44I 1696 GTGCAC G^TGCAC
ApaI 114 GGGCCC GGGCC^C
ApaLI 1696 GTGCAC G^TGCAC
AspI 3573 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4484 TGCGCA TGC^GCA
BamHI 1924 GGATCC G^GATCC
BbrPI 1590 CACGTG CAC^GTG
BbsI 299 GAAGAC GAAGAC(2/6)
BbvCI 2890 GCTGAGG CCTCAGC(-5/-2)
BlpI 196 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 196 GCTGAGC GC^T[ACGT]AGC
BpuAI 299 GAAGAC GAAGAC(2/6)
BsaAI 1590 CACGTG [CT]AC^GT[GA]
BsaI 1886 GAGACC GGTCTC(1/5)
BsaWI 3674 TCCGGA [AT]^CCGG[AT]
BseAI 3674 TCCGGA T^CCGGA
BspEI 3674 TCCGGA T^CCGGA
BspMI 1076 ACCTGC ACCTGC(4/8)
BstBI 1125 TTCGAA TT^CGAA
CelII 196 GCTGAGC GC^T[ACGT]AGC
EarI 1839 GAAGAG CTCTTC(1/4)
EcoRI 1571 GAATTC G^AATTC
EcoRV 3688 GATATC GAT^ATC
FseI 106 GGCCGGCC GGCCGG^CC
FspI 4484 TGCGCA TGC^GCA
Ksp632I 1839 GAAGAG CTCTTC(1/4)
MfeI 432 CAATTG C^AATTG
MroI 3674 TCCGGA T^CCGGA
MunI 432 CAATTG C^AATTG
NcoI 126 CCATGG C^CATGG
NspV 1125 TTCGAA TT^CGAA
PaeR7I 2154 CTCGAG C^TCGAG
PflFI 3573 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1590 CACGTG CAC^GTG
PmlI 1590 CACGTG CAC^GTG
PpuMI 983 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2702 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1718 AGTACT AGT^ACT
SexAI 3103 ACCTGGT A^CC[AT]GGT
SfiI 106 GGCCGGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1125 TTCGAA TT^CGAA
SmaI 37 CCCGGG CCC^GGG
SwaI 3998 ATTTAAAT ATTT^AAAT
Tth111I 3573 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2154 CTCGAG C^TCGAG
XmaCI 37 CCCGGG C^CCGGG
XmaI 37 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflII 221 CTTAAG C^TTAAG
482 CTTAAG
AflIII 3459 ACATGT A^C[GA][CT]GT
4162 ACATGT
AhdI 1595 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2063 GACGTGACGTC
AlwNI 1449 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
3020 CAGTGGCTG
AseI 3295 ATTAAT AT^TAAT
3587 ATTAAT
AsnI 3295 ATTAAT AT^TAAT
3587 ATTAAT
Asp700I 2189 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2395 GAAACAGTTC
AspEI 1595 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2063 GACGTGACGTC
BalI 1815 TGGCCA TGG^CCA
2022 TGGCCA
BclI 1992 TGATCA T^GATCA
2607 TGATCA
BfrI 221 CTTAAG C^TTAAG
482 CTTAAG
BsiEI 57 CGGCCG CG[GA][CT]^CG
109 CGGCCG
BsmBI 2124 GAGACG CGTCTC(1/5)
3068 GAGACG
BspLU11I 3459 ACATGT A^CATGT
4162 ACATGT
BstYI 828 AGATCC [GA]^GATC[CT]
1924 GGATCC
DraII 114 GGGCCCC [GA]G^G[ACGT]CC[CT]
983 GGGTCCT
EagI 57 CGGCCG C^GGCCG
109 CGGCCG
Eam1105I 1595 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2063 GACGTGACGTC
EclXI 57 CGGCCG C^GGCCG
109 CGGCCG
Eco52I 57 CGGCCG C^GGCCG
109 CGGCCG
EcoNI 202 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2886 CCTTGCTGAGG
EcoO109I 114 GGGCCCC [GA]G^G[ACGT]CC[CT]
983 GGGTCCT
FbaI 1992 TGATCA T^GATCA
2607 TGATCA
HaeII 2657 GGCGCT [GA]GCGC^[CT]
2821 GGCGCT
HindIII 218 AAGCTT A^AGCTT
2076 AAGCTT
HpaI 619 GTTAAC GTT^AAC
809 GTTAAC
KspI 54 CCGCGG CCGC^GG
87 CCGCGG
MflI 828 AGATCC [GA]^GATC[CT]
1924 GGATCC
MluNI 1815 TGGCCA TGG^CCA
2022 TGGCCA
MscI 1815 TGGCCA TGG^CCA
2022 TGGCCA
NaeI 107 GCCGGC GCC^GGC
1965 GCCGGC
NgoMIV 107 GCCGGC G^CCGGC
1965 GCCGGC
PshBI 3295 ATTAAT AT^TAAT
3587 ATTAAT
PstI 1062 CTGCAG CTGCA^G
1216 CTGCAG
SacII 54 CCGCGG CCGC^GG
87 CCGCGG
SphI 1917 GCATGC GCATG^C
3152 GCATGC
SspI 950 AATATT AAT^ATT
3491 AATATT
XbaI 1579 TCTAGA T^CTAGA
3380 TCTAGA
XcmI 426 CCAATGCAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1702 CCACATTCGAGTTGG
XhoII 828 AGATCC [GA]^GATC[CT]
1924 GGATCC
XmnI 2189 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2395 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I