##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BbrPI BbvCI
BclI BfrI BglI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrDI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI FspI
KpnI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 769 GTGCAC G^TGCAC
AlwNI 1387 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 769 GTGCAC G^TGCAC
Asp700I 2535 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2365 CCTAGG C^CTAGG
BamHI 2369 GGATCC G^GATCC
BcgI 665 GCACATCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1191 GTATCC GTATCC(6/5)
BlnI 2365 CCTAGG C^CTAGG
BsaAI 1188 CACGTA [CT]AC^GT[GA]
BspHI 2001 TCATGA T^CATGA
BspMI 2391 GCAGGT ACCTGC(4/8)
BsrBI 608 GAGCGG CCGCTC(-3/-3)
BstAPI 1670 GCATTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 741 TGGCCG [CT]^GGCC[GA]
EcoRI 947 GAATTC G^AATTC
HgaI 130 GACGC GACGC(5/10)
HpaI 2203 GTTAAC GTT^AAC
KspI 374 CCGCGG CCGC^GG
PshAI 641 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 158 CTGCAG CTGCA^G
RcaI 2001 TCATGA T^CATGA
SacII 374 CCGCGG CCGC^GG
SmlI 1857 CTCAAG C^T[CT][GA]AG
XbaI 2429 TCTAGA T^CTAGA
XmnI 2535 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 947 GAATTC [GA]^AATT[CT]
2284 AAATTC
AcyI 121 GGCGCC G[GA]^CG[CT]C
473 GGCGCC
ApaI 102 GGGCCC GGGCC^C
1236 GGGCCC
ApoI 947 GAATTC [GA]^AATT[CT]
2284 AAATTC
BanI 121 GGCGCC G^G[CT][GA]CC
473 GGCGCC
BbeI 121 GGCGCC GGCGC^C
473 GGCGCC
BbsI 1183 GAAGAC GAAGAC(2/6)
463 GTCTTC
BglII 1708 AGATCT A^GATCT
3025 AGATCT
BpuAI 1183 GAAGAC GAAGAC(2/6)
463 GTCTTC
BsaBI 714 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1592 GATGACTATC
BsaHI 121 GGCGCC G[GA]^CG[CT]C
473 GGCGCC
BsgI 1344 GTGCAG GTGCAG(16/14)
3017 GTGCAG
Bsp1407I 1171 TGTACA T^GTACA
2274 TGTACA
BspLU11I 642 ACATGT A^CATGT
2772 ACATGT
BsrGI 1171 TGTACA T^GTACA
2274 TGTACA
DraII 101 GGGGCCC [GA]G^G[ACGT]CC[CT]
1235 AGGGCCC
EcoO109I 101 GGGGCCC [GA]G^G[ACGT]CC[CT]
1235 AGGGCCC
HaeII 121 GGCGCC [GA]GCGC^[CT]
473 GGCGCC
Hin1I 121 GGCGCC G[GA]^CG[CT]C
473 GGCGCC
HindIII 1001 AAGCTT A^AGCTT
1827 AAGCTT
KasI 121 GGCGCC G^GCGCC
473 GGCGCC
MaeII 1189 ACGT A^CGT
1919 ACGT
MamI 714 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1592 GATGACTATC
NaeI 206 GCCGGC GCC^GGC
743 GCCGGC
NarI 121 GGCGCC GG^CGCC
473 GGCGCC
NcoI 2022 CCATGG C^CATGG
2860 CCATGG
NgoMIV 206 GCCGGC G^CCGGC
743 GCCGGC
PflMI 1253 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2855 CCACTCCATGG
PleI 68 GAGTC GAGTC(4/5)
1400 GAGTC
SapI 506 GAAGAGC GCTCTTC(1/4)
2488 GAAGAGC
SfoI 121 GGCGCC GGC^GCC
473 GGCGCC
SmaI 98 CCCGGG CCC^GGG
412 CCCGGG
SspBI 1171 TGTACA T^GTACA
2274 TGTACA
StuI 1327 AGGCCT AGG^CCT
1737 AGGCCT
Van91I 1253 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2855 CCACTCCATGG
XmaCI 98 CCCGGG C^CCGGG
412 CCCGGG
XmaI 98 CCCGGG C^CCGGG
412 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EarI
EcoNI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII