##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI AscI Asp718I AspI
AvaI BalI BamHI BanI BbeI
BbrPI BcgI BclI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI EaeI EagI
EclXI Eco47III Eco52I EcoNI FbaI
FseI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1333 GTCTAC GT^[AC][GT]AC
AflII 1405 CTTAAG C^TTAAG
AflIII 2037 ACATGT A^C[GA][CT]GT
AhdI 386 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 604 GTGCAC G^TGCAC
AocI 2267 CCTCAGG CC^T[ACGT]AGG
ApaI 19 GGGCCC GGGCC^C
ApaLI 604 GTGCAC G^TGCAC
AseI 2075 ATTAAT AT^TAAT
AsnI 2075 ATTAAT AT^TAAT
Asp700I 1298 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 386 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 604 GTGCAC G[AT]GC[AT]^C
AviII 353 TGCGCA TGC^GCA
AvrII 1280 CCTAGG C^CTAGG
BbvCI 2138 CCTCAGC CCTCAGC(-5/-2)
BcnI 572 CCCGG CC^[GC]GG
BfrI 1405 CTTAAG C^TTAAG
BglI 1929 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1229 AGATCT A^GATCT
BlnI 1280 CCTAGG C^CTAGG
BlpI 2253 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2253 GCTCAGC GC^T[ACGT]AGC
BsaAI 1183 TACGTA [CT]AC^GT[GA]
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BsiHKAI 604 GTGCAC G[AT]GC[AT]^C
BsmI 58 GCATTC GAATGC(1/-1)
BspLU11I 2037 ACATGT A^CATGT
BsrBI 205 GAGCGG CCGCTC(-3/-3)
BsrFI 325 GCCGGT [GA]^CCGG[CT]
BstAPI 1258 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2267 CCTCAGG CC^T[ACGT]AGG
CelII 2253 GCTCAGC GC^T[ACGT]AGC
Cfr10I 325 GCCGGT [GA]^CCGG[CT]
DraIII 830 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1658 GACAGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 272 CCATGG C^C[GA][CT]GG
Eam1105I 386 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2267 CCTCAGG CC^T[ACGT]AGG
EcoRI 2636 GAATTC G^AATTC
EcoRV 2488 GATATC GAT^ATC
EcoT22I 1192 ATGCAT ATGCA^T
FauI 22 CCCGC CCCGC(4/6)
FspI 353 TGCGCA TGC^GCA
HindIII 2513 AAGCTT A^AGCTT
MfeI 2053 CAATTG C^AATTG
MunI 2053 CAATTG C^AATTG
NciI 572 CCCGG CC^[GC]GG
NcoI 272 CCATGG C^CATGG
NsiI 1192 ATGCAT ATGCA^T
PpuMI 228 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2075 ATTAAT AT^TAAT
ScaI 1859 AGTACT AGT^ACT
SexAI 1337 ACCTGGT A^CC[AT]GGT
SnaBI 1183 TACGTA TAC^GTA
TfiI 918 GAATC G^A[AT]TC
XmnI 1298 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 24 CGCG CG^CG
375 CGCG
AlwNI 1905 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2090 CAGCAACTG
AvaII 229 GGACC G^G[AT]CC
1984 GGACC
BbsI 309 GAAGAC GAAGAC(2/6)
2292 GTCTTC
BfaI 1281 CTAG C^TAG
1857 CTAG
BpuAI 309 GAAGAC GAAGAC(2/6)
2292 GTCTTC
BsaI 39 GGTCTC GGTCTC(1/5)
817 GGTCTC
BseRI 2307 CTCCTC GAGGAG(10/8)
202 GAGGAG
BsgI 505 GTGCAG GTGCAG(16/14)
800 GTGCAG
BspHI 1892 TCATGA T^CATGA
2355 TCATGA
BspMI 1267 GCAGGT ACCTGC(4/8)
1997 GCAGGT
BstEII 78 GGTGACC G^GT[ACGT]ACC
328 GGTCACC
BstPI 78 GGTGACC G^GT[ACGT]ACC
328 GGTCACC
BstUI 24 CGCG CG^CG
375 CGCG
DraII 228 AGGACCT [GA]G^G[ACGT]CC[CT]
300 AGGCCCT
EarI 1423 CTCTTC CTCTTC(1/4)
1561 CTCTTC
EcoO109I 228 AGGACCT [GA]G^G[ACGT]CC[CT]
300 AGGCCCT
EcoO65I 78 GGTGACC G^GT[ACGT]ACC
328 GGTCACC
EcoT14I 272 CCATGG C^C[AT][AT]GG
1280 CCTAGG
Ksp632I 1423 CTCTTC CTCTTC(1/4)
1561 CTCTTC
MaeI 1281 CTAG C^TAG
1857 CTAG
MslI 597 CACGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
621 CATGCGGATG
MspA1I 152 CAGCTG C[AC]G^C[GT]G
1428 CAGCTG
MvnI 24 CGCG CG^CG
375 CGCG
PflMI 124 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2167 CCAAAGACTGG
PstI 155 CTGCAG CTGCA^G
2315 CTGCAG
PvuII 152 CAGCTG CAG^CTG
1428 CAGCTG
RcaI 1892 TCATGA T^CATGA
2355 TCATGA
SspI 433 AATATT AAT^ATT
1821 AATATT
StyI 272 CCATGG C^C[AT][AT]GG
1280 CCTAGG
TaqI 401 TCGA T^CGA
447 TCGA
TthHB8I 401 TCGA T^CGA
447 TCGA
Van91I 124 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2167 CCAAAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BciVI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII