##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccII AclI AcsI AflII
AgeI AhdI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BanII BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstUI BstXI CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tsp509I Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 307 GGTACC G^GTACC
AccI 1114 GTATAC GT^[AC][GT]AC
AccIII 40 TCCGGA T^CCGGA
AcyI 1058 GGCGCC G[GA]^CG[CT]C
Aor51HI 540 AGCGCT AGC^GCT
Asp718I 307 GGTACC G^GTACC
BalI 726 TGGCCA TGG^CCA
BamHI 373 GGATCC G^GATCC
BbeI 1058 GGCGCC GGCGC^C
BbrPI 178 CACGTG CAC^GTG
BbsI 895 GAAGAC GAAGAC(2/6)
BclI 780 TGATCA T^GATCA
BglI 117 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 812 CTGGAG CTGGAG(16/14)
BpuAI 895 GAAGAC GAAGAC(2/6)
BsaAI 178 CACGTG [CT]AC^GT[GA]
BsaHI 1058 GGCGCC G[GA]^CG[CT]C
BsaI 556 GAGACC GGTCTC(1/5)
BsaWI 40 TCCGGA [AT]^CCGG[AT]
BseAI 40 TCCGGA T^CCGGA
BsmAI 556 GAGAC GTCTC(1/5)
BspEI 40 TCCGGA T^CCGGA
BspLU11I 1051 ACATGT A^CATGT
Bst1107I 1114 GTATAC GTA^TAC
BstAPI 217 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 298 GGTGACC G^GT[ACGT]ACC
BstPI 298 GGTGACC G^GT[ACGT]ACC
BstYI 373 GGATCC [GA]^GATC[CT]
BstZ17I 1114 GTATAC GTA^TAC
EaeI 726 TGGCCA [CT]^GGCC[GA]
Eco47III 540 AGCGCT AGC^GCT
EcoNI 311 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 298 GGTGACC G^GT[ACGT]ACC
EcoT22I 939 ATGCAT ATGCA^T
FauI 751 GCGGG CCCGC(4/6)
FbaI 780 TGATCA T^GATCA
HgaI 226 GCGTC GACGC(5/10)
Hin1I 1058 GGCGCC G[GA]^CG[CT]C
KasI 1058 GGCGCC G^GCGCC
KpnI 307 GGTACC GGTAC^C
MflI 373 GGATCC [GA]^GATC[CT]
MluNI 726 TGGCCA TGG^CCA
MroI 40 TCCGGA T^CCGGA
MscI 726 TGGCCA TGG^CCA
MseI 341 TTAA T^TAA
MslI 292 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1058 GGCGCC GG^CGCC
NsiI 939 ATGCAT ATGCA^T
PmaCI 178 CACGTG CAC^GTG
PmlI 178 CACGTG CAC^GTG
PstI 215 CTGCAG CTGCA^G
SfcI 215 CTGCAG C^T[GA][CT]AG
SfoI 1058 GGCGCC GGC^GCC
SmlI 517 CTCAAG C^T[CT][GA]AG
SphI 111 GCATGC GCATG^C
TaqI 1099 TCGA T^CGA
Tru9I 341 TTAA T^TAA
TthHB8I 1099 TCGA T^CGA
XhoII 373 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 455 CCGC CCGC(-3/-1)
751 GCGG
AfaI 275 GTAC GT^AC
308 GTAC
AlwI 374 GATCC GGATC(4/5)
373 GGATC
AvaI 153 CCCGGG C^[CT]CG[GA]G
879 CCCGGG
BsoBI 153 CCCGGG C^[CT]CG[GA]G
879 CCCGGG
EarI 689 CTCTTC CTCTTC(1/4)
347 GAAGAG
HaeII 540 AGCGCT [GA]GCGC^[CT]
1058 GGCGCC
HinfI 211 GACTC G^A[ACGT]TC
659 GAGTC
Ksp632I 689 CTCTTC CTCTTC(1/4)
347 GAAGAG
NspI 111 GCATGC [GA]CATG^[CT]
1051 ACATGT
PflMI 280 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
537 CCAAGCGCTGG
PleI 211 GACTC GAGTC(4/5)
659 GAGTC
PvuII 575 CAGCTG CAG^CTG
650 CAGCTG
RsaI 275 GTAC GT^AC
308 GTAC
SapI 347 GAAGAGC GCTCTTC(1/4)
688 GCTCTTC
SmaI 153 CCCGGG CCC^GGG
879 CCCGGG
StuI 195 AGGCCT AGG^CCT
760 AGGCCT
Van91I 280 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
537 CCAAGCGCTGG
XmaCI 153 CCCGGG C^CCGGG
879 CCCGGG
XmaI 153 CCCGGG C^CCGGG
879 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AflIII AluI AlwNI AocI AvaII
BanI BbvI BcnI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI