##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BciVI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BseRI BsiWI BsmI Bsp1407I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HpaI KasI KpnI Ksp632I MfeI
MflI MluI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PstI PvuI RsrII SacI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 4 GTCGAC GT^[AC][GT]AC
AclI 554 AACGTT AA^CGTT
AfaI 153 GTAC GT^AC
AflIII 981 ACATGT A^C[GA][CT]GT
Alw44I 188 GTGCAC G^TGCAC
ApaLI 188 GTGCAC G^TGCAC
AseI 377 ATTAAT AT^TAAT
AsnI 377 ATTAAT AT^TAAT
AvaI 1172 CTCGAG C^[CT]CG[GA]G
BalI 883 TGGCCA TGG^CCA
BbvCI 529 CCTCAGC CCTCAGC(-5/-2)
BcgI 1105 GCAAGATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 661 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 328 CTGGAG CTGGAG(16/14)
BsaBI 539 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 808 GGTCTC GGTCTC(1/5)
BsoBI 1172 CTCGAG C^[CT]CG[GA]G
BspDI 1136 ATCGAT AT^CGAT
BspHI 229 TCATGA T^CATGA
BspLU11I 981 ACATGT A^CATGT
BspMI 805 GCAGGT ACCTGC(4/8)
BsrDI 315 GCAATG GCAATG(2/0)
BstAPI 1116 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 384 GCGC GCG^C
ClaI 1136 ATCGAT AT^CGAT
DraIII 182 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 138 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 384 GCGC GCG^C
HinP1I 384 GCGC G^CGC
HincII 4 GTCGAC GT[CT]^[GA]AC
HindII 4 GTCGAC GT[CT]^[GA]AC
KspI 922 CCGCGG CCGC^GG
MaeII 555 ACGT A^CGT
MamI 539 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 883 TGGCCA TGG^CCA
MscI 883 TGGCCA TGG^CCA
MslI 1316 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 990 CCATGG C^CATGG
NspI 981 ACATGT [GA]CATG^[CT]
PaeR7I 1172 CTCGAG C^TCGAG
PshAI 934 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 377 ATTAAT AT^TAAT
Psp1406I 554 AACGTT AA^CGTT
RcaI 229 TCATGA T^CATGA
RsaI 153 GTAC GT^AC
SacII 922 CCGCGG CCGC^GG
SalI 4 GTCGAC G^TCGAC
XhoI 1172 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 45 TCCGGA T^CCGGA
1194 TCCGGA
AcsI 422 AAATTT [GA]^AATT[CT]
1118 AAATTT
ApoI 422 AAATTT [GA]^AATT[CT]
1118 AAATTT
BanII 38 GAGCCC G[GA]GC[CT]^C
273 GGGCTC
BbsI 820 GAAGAC GAAGAC(2/6)
1177 GTCTTC
BpuAI 820 GAAGAC GAAGAC(2/6)
1177 GTCTTC
BsaWI 45 TCCGGA [AT]^CCGG[AT]
1194 TCCGGA
BseAI 45 TCCGGA T^CCGGA
1194 TCCGGA
BsgI 1058 CTGCAC GTGCAG(16/14)
803 GTGCAG
BsiEI 286 CGACCG CG[GA][CT]^CG
1249 CGACCG
BsmBI 940 CGTCTC CGTCTC(1/5)
1052 GAGACG
BsmFI 171 GTCCC GGGAC(10/14)
406 GTCCC
BspEI 45 TCCGGA T^CCGGA
1194 TCCGGA
BsrBI 31 CCGCTC CCGCTC(-3/-3)
836 CCGCTC
DrdI 287 GACCGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1168 GACCCTCGAGTC
EaeI 883 TGGCCA [CT]^GGCC[GA]
1245 TGGCCG
HapII 46 CCGG C^CGG
1195 CCGG
HindIII 724 AAGCTT A^AGCTT
1428 AAGCTT
HpaII 46 CCGG C^CGG
1195 CCGG
MroI 45 TCCGGA T^CCGGA
1194 TCCGGA
MspI 46 CCGG C^CGG
1195 CCGG
PvuII 671 CAGCTG CAG^CTG
1255 CAGCTG
TfiI 24 GAATC G^A[AT]TC
514 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I