################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseRI BsiWI BsmI Bsp1407I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI Ksp632I MfeI MflI MluI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PstI PvuI RsrII SacI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 4 GTCGAC GT^[AC][GT]AC AclI 554 AACGTT AA^CGTT AfaI 153 GTAC GT^AC AflIII 981 ACATGT A^C[GA][CT]GT Alw44I 188 GTGCAC G^TGCAC ApaLI 188 GTGCAC G^TGCAC AseI 377 ATTAAT AT^TAAT AsnI 377 ATTAAT AT^TAAT AvaI 1172 CTCGAG C^[CT]CG[GA]G BalI 883 TGGCCA TGG^CCA BbvCI 529 CCTCAGC CCTCAGC(-5/-2) BcgI 1105 GCAAGATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 661 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 328 CTGGAG CTGGAG(16/14) BsaBI 539 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 808 GGTCTC GGTCTC(1/5) BsoBI 1172 CTCGAG C^[CT]CG[GA]G BspDI 1136 ATCGAT AT^CGAT BspHI 229 TCATGA T^CATGA BspLU11I 981 ACATGT A^CATGT BspMI 805 GCAGGT ACCTGC(4/8) BsrDI 315 GCAATG GCAATG(2/0) BstAPI 1116 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 384 GCGC GCG^C ClaI 1136 ATCGAT AT^CGAT DraIII 182 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 138 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HhaI 384 GCGC GCG^C HinP1I 384 GCGC G^CGC HincII 4 GTCGAC GT[CT]^[GA]AC HindII 4 GTCGAC GT[CT]^[GA]AC KspI 922 CCGCGG CCGC^GG MaeII 555 ACGT A^CGT MamI 539 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 883 TGGCCA TGG^CCA MscI 883 TGGCCA TGG^CCA MslI 1316 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 990 CCATGG C^CATGG NspI 981 ACATGT [GA]CATG^[CT] PaeR7I 1172 CTCGAG C^TCGAG PshAI 934 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 377 ATTAAT AT^TAAT Psp1406I 554 AACGTT AA^CGTT RcaI 229 TCATGA T^CATGA RsaI 153 GTAC GT^AC SacII 922 CCGCGG CCGC^GG SalI 4 GTCGAC G^TCGAC XhoI 1172 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 45 TCCGGA T^CCGGA 1194 TCCGGA AcsI 422 AAATTT [GA]^AATT[CT] 1118 AAATTT ApoI 422 AAATTT [GA]^AATT[CT] 1118 AAATTT BanII 38 GAGCCC G[GA]GC[CT]^C 273 GGGCTC BbsI 820 GAAGAC GAAGAC(2/6) 1177 GTCTTC BpuAI 820 GAAGAC GAAGAC(2/6) 1177 GTCTTC BsaWI 45 TCCGGA [AT]^CCGG[AT] 1194 TCCGGA BseAI 45 TCCGGA T^CCGGA 1194 TCCGGA BsgI 1058 CTGCAC GTGCAG(16/14) 803 GTGCAG BsiEI 286 CGACCG CG[GA][CT]^CG 1249 CGACCG BsmBI 940 CGTCTC CGTCTC(1/5) 1052 GAGACG BsmFI 171 GTCCC GGGAC(10/14) 406 GTCCC BspEI 45 TCCGGA T^CCGGA 1194 TCCGGA BsrBI 31 CCGCTC CCGCTC(-3/-3) 836 CCGCTC DrdI 287 GACCGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1168 GACCCTCGAGTC EaeI 883 TGGCCA [CT]^GGCC[GA] 1245 TGGCCG HapII 46 CCGG C^CGG 1195 CCGG HindIII 724 AAGCTT A^AGCTT 1428 AAGCTT HpaII 46 CCGG C^CGG 1195 CCGG MroI 45 TCCGGA T^CCGGA 1194 TCCGGA MspI 46 CCGG C^CGG 1195 CCGG PvuII 671 CAGCTG CAG^CTG 1255 CAGCTG TfiI 24 GAATC G^A[AT]TC 514 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I