##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI BalI
BbrPI BbsI BcgI BciVI BfrI
BlpI Bpu1102I BpuAI BsaWI BseAI
BsiHKAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoT22I HincII HindII
HindIII HpaI KpnI MfeI MluI
MluNI MroI MscI MunI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
SexAI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AlwNI 1532 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 64 GGGCCC GGGCC^C
Asp700I 1898 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 543 CCCGAG C^[CT]CG[GA]G
AviII 1279 TGCGCA TGC^GCA
AvrII 808 CCTAGG C^CTAGG
BamHI 134 GGATCC G^GATCC
BanI 278 GGCGCC G^G[CT][GA]CC
BbeI 278 GGCGCC GGCGC^C
BbvCI 786 CCTCAGC CCTCAGC(-5/-2)
BclI 901 TGATCA T^GATCA
BglI 66 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 808 CCTAGG C^CTAGG
BsaAI 609 CACGTA [CT]AC^GT[GA]
BsaI 960 GAGACC GGTCTC(1/5)
BsiEI 69 CGGCCG CG[GA][CT]^CG
BsmFI 17 GTCCC GGGAC(10/14)
BsoBI 543 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2456 TGTACA T^GTACA
BsrDI 1521 CATTGC GCAATG(2/0)
BsrFI 71 GCCGGC [GA]^CCGG[CT]
BsrGI 2456 TGTACA T^GTACA
BstXI 120 CCACACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 71 GCCGGC [GA]^CCGG[CT]
DsaI 60 CCGCGG C^C[GA][CT]GG
EaeI 69 CGGCCG [CT]^GGCC[GA]
EagI 69 CGGCCG C^GGCCG
EclXI 69 CGGCCG C^GGCCG
Eco52I 69 CGGCCG C^GGCCG
EcoRI 1002 GAATTC G^AATTC
EcoT14I 808 CCTAGG C^C[AT][AT]GG
FbaI 901 TGATCA T^GATCA
FseI 70 GGCCGGCC GGCCGG^CC
FspI 1279 TGCGCA TGC^GCA
KasI 278 GGCGCC G^GCGCC
KspI 60 CCGCGG CCGC^GG
MaeII 610 ACGT A^CGT
NaeI 71 GCCGGC GCC^GGC
NarI 278 GGCGCC GG^CGCC
NdeI 1444 CATATG CA^TATG
NgoMIV 71 GCCGGC G^CCGGC
NspI 975 GCATGC [GA]CATG^[CT]
PpuMI 15 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 789 CAGCTG CAG^CTG
SacII 60 CCGCGG CCGC^GG
SapI 1648 GCTCTTC GCTCTTC(1/4)
SfiI 65 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 278 GGCGCC GGC^GCC
SphI 975 GCATGC GCATG^C
SspBI 2456 TGTACA T^GTACA
SspI 1930 AATATT AAT^ATT
StuI 177 AGGCCT AGG^CCT
StyI 808 CCTAGG C^C[AT][AT]GG
XcmI 915 CCAGTAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1898 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 85 GGCGTC G[GA]^CG[CT]C
278 GGCGCC
AocI 80 CCTCAGG CC^T[ACGT]AGG
1395 CCTAAGG
BanII 64 GGGCCC G[GA]GC[CT]^C
720 GGGCTC
BglII 863 AGATCT A^GATCT
1066 AGATCT
BsaBI 1118 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2315 GATGTAAATC
BsaHI 85 GGCGTC G[GA]^CG[CT]C
278 GGCGCC
BsmAI 960 GAGAC GTCTC(1/5)
2274 GAGAC
Bsu36I 80 CCTCAGG CC^T[ACGT]AGG
1395 CCTAAGG
DraII 15 AGGTCCC [GA]G^G[ACGT]CC[CT]
558 AGGCCCT
Eco81I 80 CCTCAGG CC^T[ACGT]AGG
1395 CCTAAGG
EcoO109I 15 AGGTCCC [GA]G^G[ACGT]CC[CT]
558 AGGCCCT
EcoRV 1362 GATATC GAT^ATC
1809 GATATC
HaeII 278 GGCGCC [GA]GCGC^[CT]
361 GGCGCT
HgaI 86 GCGTC GACGC(5/10)
91 GCGTC
Hin1I 85 GGCGTC G[GA]^CG[CT]C
278 GGCGCC
MamI 1118 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2315 GATGTAAATC
PflMI 1424 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1814 CCATAAGCTGG
PleI 2082 GAGTC GAGTC(4/5)
2252 GAGTC
ScaI 1915 AGTACT AGT^ACT
1923 AGTACT
Van91I 1424 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1814 CCATAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmI Bsp1286I
BspHI BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoNI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII