##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AviII BbeI BbrPI
BciVI BclI BfrI BglI BglII
BsaAI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI FbaI FseI FspI HpaI
KasI KspI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacI SacII SalI SapI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2284 GGTACC G^GTACC
AccI 1071 GTCTAC GT^[AC][GT]AC
AcyI 12 GGCGTC G[GA]^CG[CT]C
Alw44I 1773 GTGCAC G^TGCAC
AocI 570 CCTAAGG CC^T[ACGT]AGG
ApaI 448 GGGCCC GGGCC^C
ApaLI 1773 GTGCAC G^TGCAC
Asp718I 2284 GGTACC G^GTACC
AvrII 139 CCTAGG C^CTAGG
BcgI 1991 CGACTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 139 CCTAGG C^CTAGG
BlpI 902 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 902 GCTGAGC GC^T[ACGT]AGC
BsaBI 2663 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 12 GGCGTC G[GA]^CG[CT]C
BsaI 2571 GAGACC GGTCTC(1/5)
BsgI 1571 CTGCAC GTGCAG(16/14)
BsrDI 2429 CATTGC GCAATG(2/0)
BsrFI 1255 ACCGGC [GA]^CCGG[CT]
BssSI 1332 CACGAG CACGAG(-5/-1)
BstAPI 1202 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 570 CCTAAGG CC^T[ACGT]AGG
CelII 902 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1255 ACCGGC [GA]^CCGG[CT]
DrdI 1278 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 570 CCTAAGG CC^T[ACGT]AGG
EcoRV 2364 GATATC GAT^ATC
EcoT22I 2137 ATGCAT ATGCA^T
HgaI 13 GCGTC GACGC(5/10)
Hin1I 12 GGCGTC G[GA]^CG[CT]C
HindIII 702 AAGCTT A^AGCTT
KpnI 2284 GGTACC GGTAC^C
MamI 2663 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 772 CAATTG C^AATTG
MunI 772 CAATTG C^AATTG
NheI 2535 GCTAGC G^CTAGC
NsiI 2137 ATGCAT ATGCA^T
NspI 2404 ACATGC [GA]CATG^[CT]
PshAI 1316 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1457 CCTGCAGG CCTGCA^GG
Sse8387I 1457 CCTGCAGG CCTGCA^GG
SspI 1710 AATATT AAT^ATT
StuI 91 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 1107 AACGTT AA^CGTT
2391 AACGTT
AcsI 40 GAATTT [GA]^AATT[CT]
2346 AAATTT
ApoI 40 GAATTT [GA]^AATT[CT]
2346 AAATTT
Asp700I 335 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2459 GAAGGACTTC
BbvCI 1355 CCTCAGC CCTCAGC(-5/-2)
2580 GCTGAGG
BsiEI 1493 CGACCG CG[GA][CT]^CG
2672 CGACCG
BspHI 1531 TCATGA T^CATGA
2368 TCATGA
BspMI 1207 ACCTGC ACCTGC(4/8)
2760 GCAGGT
BstBI 1449 TTCGAA TT^CGAA
1705 TTCGAA
DraIII 1718 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2276 CACCAAGTG
HaeII 358 AGCGCC [GA]GCGC^[CT]
1965 AGCGCC
NcoI 1637 CCATGG C^CATGG
1899 CCATGG
NspV 1449 TTCGAA TT^CGAA
1705 TTCGAA
Psp1406I 1107 AACGTT AA^CGTT
2391 AACGTT
RcaI 1531 TCATGA T^CATGA
2368 TCATGA
ScaI 1468 AGTACT AGT^ACT
1540 AGTACT
SfuI 1449 TTCGAA TT^CGAA
1705 TTCGAA
SmlI 492 CTCAAG C^T[CT][GA]AG
1030 CTCAAG
TfiI 192 GAATC G^A[AT]TC
528 GATTC
XmnI 335 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2459 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII