##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BlnI BsaAI BsaHI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eam1105I EcoNI EcoO65I EcoRV
FseI FspI Hin1I KasI KpnI
MluI MroI NarI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI ScaI SfoI SfuI SgrAI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Aor51HI 123 AGCGCT AGC^GCT
Asp700I 6969 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 3859 GCTGAGG CCTCAGC(-5/-2)
BciVI 3351 GTATCC GTATCC(6/5)
BclI 1483 TGATCA T^GATCA
BsaBI 6630 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2305 TCCGGT [AT]^CCGG[AT]
BsmBI 3789 CGTCTC CGTCTC(1/5)
BsrFI 158 GCCGGC [GA]^CCGG[CT]
BssSI 2561 CACGAG CACGAG(-5/-1)
Cfr10I 158 GCCGGC [GA]^CCGG[CT]
DrdI 405 GACCCCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 123 AGCGCT AGC^GCT
EcoT22I 3020 ATGCAT ATGCA^T
FbaI 1483 TGATCA T^GATCA
HaeII 123 AGCGCT [GA]GCGC^[CT]
HgaI 105 GACGC GACGC(5/10)
HpaI 1503 GTTAAC GTT^AAC
MamI 6630 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 158 GCCGGC GCC^GGC
NdeI 3023 CATATG CA^TATG
NgoMIV 158 GCCGGC G^CCGGC
NsiI 3020 ATGCAT ATGCA^T
PshAI 731 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1898 CCTGCAGG CCTGCA^GG
SfiI 2 GGCCGACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1814 ACTAGT A^CTAGT
Sse8387I 1898 CCTGCAGG CCTGCA^GG
XcmI 7239 CCAGTTTACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 6969 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 47 enzymes) #
###################################
AflII 3056 CTTAAG C^TTAAG
5504 CTTAAG
AocI 450 CCTGAGG CC^T[ACGT]AGG
1733 CCTGAGG
AseI 5762 ATTAAT AT^TAAT
5855 ATTAAT
AsnI 5762 ATTAAT AT^TAAT
5855 ATTAAT
AspI 3784 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6356 GACTATGTC
BalI 1459 TGGCCA TGG^CCA
2291 TGGCCA
BbsI 1198 GTCTTC GAAGAC(2/6)
2454 GTCTTC
BcgI 32 CGACGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
959 GCACAATAATCG
BfrI 3056 CTTAAG C^TTAAG
5504 CTTAAG
BglI 3 GCCGACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3907 GCCGAGGTGGC
BlpI 1560 GCTCAGC GC^T[ACGT]AGC
6030 GCTAAGC
Bpu1102I 1560 GCTCAGC GC^T[ACGT]AGC
6030 GCTAAGC
BpuAI 1198 GTCTTC GAAGAC(2/6)
2454 GTCTTC
BsiEI 1 CGGCCG CG[GA][CT]^CG
160 CGGCCG
BsmI 792 GAATGC GAATGC(1/-1)
129 GCATTC
Bsp1407I 3263 TGTACA T^GTACA
5791 TGTACA
BspHI 4232 TCATGA T^CATGA
7140 TCATGA
BspLU11I 5741 ACATGT A^CATGT
7437 ACATGT
BsrBI 327 CCGCTC CCGCTC(-3/-3)
185 GAGCGG
BsrDI 2372 CATTGC GCAATG(2/0)
1837 GCAATG
BsrGI 3263 TGTACA T^GTACA
5791 TGTACA
BstAPI 1892 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3891 GCAGAAGCTGC
BstXI 1876 CCAAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3709 CCAGCACTTTGG
Bsu36I 450 CCTGAGG CC^T[ACGT]AGG
1733 CCTGAGG
CelII 1560 GCTCAGC GC^T[ACGT]AGC
6030 GCTAAGC
DraIII 219 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3520 CACCCAGTG
EagI 1 CGGCCG C^GGCCG
160 CGGCCG
EclXI 1 CGGCCG C^GGCCG
160 CGGCCG
Eco52I 1 CGGCCG C^GGCCG
160 CGGCCG
Eco81I 450 CCTGAGG CC^T[ACGT]AGG
1733 CCTGAGG
EcoRI 4244 GAATTC G^AATTC
6510 GAATTC
HindIII 1669 AAGCTT A^AGCTT
1722 AAGCTT
KspI 25 CCGCGG CCGC^GG
2120 CCGCGG
MfeI 907 CAATTG C^AATTG
1267 CAATTG
MluNI 1459 TGGCCA TGG^CCA
2291 TGGCCA
MscI 1459 TGGCCA TGG^CCA
2291 TGGCCA
MunI 907 CAATTG C^AATTG
1267 CAATTG
NheI 6447 GCTAGC G^CTAGC
7253 GCTAGC
PflFI 3784 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6356 GACTATGTC
PshBI 5762 ATTAAT AT^TAAT
5855 ATTAAT
RcaI 4232 TCATGA T^CATGA
7140 TCATGA
SacII 25 CCGCGG CCGC^GG
2120 CCGCGG
SapI 4178 GAAGAGC GCTCTTC(1/4)
6181 GCTCTTC
SexAI 295 ACCTGGT A^CC[AT]GGT
1516 ACCAGGT
SphI 724 GCATGC GCATG^C
3830 GCATGC
SspBI 3263 TGTACA T^GTACA
5791 TGTACA
Tth111I 3784 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
6356 GACTATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmaCI XmaI