##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI BbeI
BciVI BsaBI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I Eco47III EcoRV
FseI HindIII HpaI KasI KpnI
MamI MfeI MluI MunI NarI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI PvuI RsrII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 4538 GACGTC GACGT^C
AclI 472 AACGTT AA^CGTT
AflII 6801 CTTAAG C^TTAAG
Asp700I 6541 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1056 TGCGCA TGC^GCA
AvrII 7162 CCTAGG C^CTAGG
BamHI 5089 GGATCC G^GATCC
BcgI 528 GCAGGGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6801 CTTAAG C^TTAAG
BglII 3022 AGATCT A^GATCT
BlnI 7162 CCTAGG C^CTAGG
BsiEI 1001 CGGCCG CG[GA][CT]^CG
BspLU11I 3228 ACATGT A^CATGT
BsrBI 1036 GAGCGG CCGCTC(-3/-3)
BstEII 6416 GGTGACC G^GT[ACGT]ACC
BstPI 6416 GGTGACC G^GT[ACGT]ACC
DraIII 7034 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1001 CGGCCG C^GGCCG
EclXI 1001 CGGCCG C^GGCCG
Eco52I 1001 CGGCCG C^GGCCG
EcoO65I 6416 GGTGACC G^GT[ACGT]ACC
EcoT22I 2983 ATGCAT ATGCA^T
FspI 1056 TGCGCA TGC^GCA
KspI 14 CCGCGG CCGC^GG
NaeI 4978 GCCGGC GCC^GGC
NgoMIV 4978 GCCGGC G^CCGGC
NheI 1789 GCTAGC G^CTAGC
NsiI 2983 ATGCAT ATGCA^T
PaeR7I 6361 CTCGAG C^TCGAG
Psp1406I 472 AACGTT AA^CGTT
SacII 14 CCGCGG CCGC^GG
SbfI 579 CCTGCAGG CCTGCA^GG
ScaI 4927 AGTACT AGT^ACT
SphI 4507 GCATGC GCATG^C
Sse8387I 579 CCTGCAGG CCTGCA^GG
XbaI 4433 TCTAGA T^CTAGA
XhoI 6361 CTCGAG C^TCGAG
XmnI 6541 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 4770 GTAGAC GT^[AC][GT]AC
5497 GTAGAC
AccIII 132 TCCGGA T^CCGGA
1097 TCCGGA
AcyI 4538 GACGTC G[GA]^CG[CT]C
6299 GGCGTC
AflIII 3153 ACGTGT A^C[GA][CT]GT
3228 ACATGT
Alw44I 3517 GTGCAC G^TGCAC
5865 GTGCAC
ApaLI 3517 GTGCAC G^TGCAC
5865 GTGCAC
AspI 1015 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2604 GACAGGGTC
BbrPI 1613 CACGTG CAC^GTG
3152 CACGTG
BbvCI 3895 GCTGAGG CCTCAGC(-5/-2)
5763 GCTGAGG
BclI 1573 TGATCA T^GATCA
2099 TGATCA
BsaHI 4538 GACGTC G[GA]^CG[CT]C
6299 GGCGTC
BseAI 132 TCCGGA T^CCGGA
1097 TCCGGA
BsmBI 1677 CGTCTC CGTCTC(1/5)
3758 CGTCTC
BspEI 132 TCCGGA T^CCGGA
1097 TCCGGA
BsrDI 1577 CATTGC GCAATG(2/0)
2930 CATTGC
BssSI 1558 CTCGTG CACGAG(-5/-1)
4054 CTCGTG
DraI 3017 TTTAAA TTT^AAA
3956 TTTAAA
DrdI 3716 GACTGCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6777 GACCCCTCTGTC
EcoRI 2762 GAATTC G^AATTC
4281 GAATTC
FbaI 1573 TGATCA T^GATCA
2099 TGATCA
Hin1I 4538 GACGTC G[GA]^CG[CT]C
6299 GGCGTC
HincII 1926 GTTGAC GT[CT]^[GA]AC
2457 GTTGAC
HindII 1926 GTTGAC GT[CT]^[GA]AC
2457 GTTGAC
MroI 132 TCCGGA T^CCGGA
1097 TCCGGA
NdeI 4078 CATATG CA^TATG
4475 CATATG
PflFI 1015 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2604 GACAGGGTC
PmaCI 1613 CACGTG CAC^GTG
3152 CACGTG
PmlI 1613 CACGTG CAC^GTG
3152 CACGTG
SapI 3364 GCTCTTC GCTCTTC(1/4)
3380 GCTCTTC
SmaI 434 CCCGGG CCC^GGG
816 CCCGGG
Tth111I 1015 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2604 GACAGGGTC
XmaCI 434 CCCGGG C^CCGGG
816 CCCGGG
XmaI 434 CCCGGG C^CCGGG
816 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII