################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI BbeI BciVI BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I Eco47III EcoRV FseI HindIII HpaI KasI KpnI MamI MfeI MluI MunI NarI NotI NruI NspV PacI PinAI PmeI PshAI PshBI PvuI RsrII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 4538 GACGTC GACGT^C AclI 472 AACGTT AA^CGTT AflII 6801 CTTAAG C^TTAAG Asp700I 6541 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1056 TGCGCA TGC^GCA AvrII 7162 CCTAGG C^CTAGG BamHI 5089 GGATCC G^GATCC BcgI 528 GCAGGGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 6801 CTTAAG C^TTAAG BglII 3022 AGATCT A^GATCT BlnI 7162 CCTAGG C^CTAGG BsiEI 1001 CGGCCG CG[GA][CT]^CG BspLU11I 3228 ACATGT A^CATGT BsrBI 1036 GAGCGG CCGCTC(-3/-3) BstEII 6416 GGTGACC G^GT[ACGT]ACC BstPI 6416 GGTGACC G^GT[ACGT]ACC DraIII 7034 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1001 CGGCCG C^GGCCG EclXI 1001 CGGCCG C^GGCCG Eco52I 1001 CGGCCG C^GGCCG EcoO65I 6416 GGTGACC G^GT[ACGT]ACC EcoT22I 2983 ATGCAT ATGCA^T FspI 1056 TGCGCA TGC^GCA KspI 14 CCGCGG CCGC^GG NaeI 4978 GCCGGC GCC^GGC NgoMIV 4978 GCCGGC G^CCGGC NheI 1789 GCTAGC G^CTAGC NsiI 2983 ATGCAT ATGCA^T PaeR7I 6361 CTCGAG C^TCGAG Psp1406I 472 AACGTT AA^CGTT SacII 14 CCGCGG CCGC^GG SbfI 579 CCTGCAGG CCTGCA^GG ScaI 4927 AGTACT AGT^ACT SphI 4507 GCATGC GCATG^C Sse8387I 579 CCTGCAGG CCTGCA^GG XbaI 4433 TCTAGA T^CTAGA XhoI 6361 CTCGAG C^TCGAG XmnI 6541 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 4770 GTAGAC GT^[AC][GT]AC 5497 GTAGAC AccIII 132 TCCGGA T^CCGGA 1097 TCCGGA AcyI 4538 GACGTC G[GA]^CG[CT]C 6299 GGCGTC AflIII 3153 ACGTGT A^C[GA][CT]GT 3228 ACATGT Alw44I 3517 GTGCAC G^TGCAC 5865 GTGCAC ApaLI 3517 GTGCAC G^TGCAC 5865 GTGCAC AspI 1015 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2604 GACAGGGTC BbrPI 1613 CACGTG CAC^GTG 3152 CACGTG BbvCI 3895 GCTGAGG CCTCAGC(-5/-2) 5763 GCTGAGG BclI 1573 TGATCA T^GATCA 2099 TGATCA BsaHI 4538 GACGTC G[GA]^CG[CT]C 6299 GGCGTC BseAI 132 TCCGGA T^CCGGA 1097 TCCGGA BsmBI 1677 CGTCTC CGTCTC(1/5) 3758 CGTCTC BspEI 132 TCCGGA T^CCGGA 1097 TCCGGA BsrDI 1577 CATTGC GCAATG(2/0) 2930 CATTGC BssSI 1558 CTCGTG CACGAG(-5/-1) 4054 CTCGTG DraI 3017 TTTAAA TTT^AAA 3956 TTTAAA DrdI 3716 GACTGCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6777 GACCCCTCTGTC EcoRI 2762 GAATTC G^AATTC 4281 GAATTC FbaI 1573 TGATCA T^GATCA 2099 TGATCA Hin1I 4538 GACGTC G[GA]^CG[CT]C 6299 GGCGTC HincII 1926 GTTGAC GT[CT]^[GA]AC 2457 GTTGAC HindII 1926 GTTGAC GT[CT]^[GA]AC 2457 GTTGAC MroI 132 TCCGGA T^CCGGA 1097 TCCGGA NdeI 4078 CATATG CA^TATG 4475 CATATG PflFI 1015 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2604 GACAGGGTC PmaCI 1613 CACGTG CAC^GTG 3152 CACGTG PmlI 1613 CACGTG CAC^GTG 3152 CACGTG SapI 3364 GCTCTTC GCTCTTC(1/4) 3380 GCTCTTC SmaI 434 CCCGGG CCC^GGG 816 CCCGGG Tth111I 1015 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2604 GACAGGGTC XmaCI 434 CCCGGG C^CCGGG 816 CCCGGG XmaI 434 CCCGGG C^CCGGG 816 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII