##################################
# NO CUT ENZYMES ( 25 enzymes) #
##################################
AscI AvrII BcgI BciVI BlnI
BsiEI BsiWI CpoI EagI EclXI
Eco52I FseI MluI NdeI NheI
NotI NruI PacI PmeI PvuI
RsrII SalI SgrAI SnaBI XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 6760 AACGTT AA^CGTT
AflII 4852 CTTAAG C^TTAAG
AgeI 2219 ACCGGT A^CCGGT
AhdI 464 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5027 AGCGCT AGC^GCT
AspEI 464 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1275 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 27 GGCGCC GGCGC^C
BbrPI 2713 CACGTG CAC^GTG
BfrI 4852 CTTAAG C^TTAAG
BsaAI 2713 CACGTG [CT]AC^GT[GA]
BspDI 2905 ATCGAT AT^CGAT
BsrDI 743 GCAATG GCAATG(2/0)
BssHII 1303 GCGCGC G^CGCGC
BssSI 3859 CACGAG CACGAG(-5/-1)
Bst1107I 1171 GTATAC GTA^TAC
BstEII 1248 GGTCACC G^GT[ACGT]ACC
BstPI 1248 GGTCACC G^GT[ACGT]ACC
BstZ17I 1171 GTATAC GTA^TAC
ClaI 2905 ATCGAT AT^CGAT
DrdI 3532 GACATGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 464 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5027 AGCGCT AGC^GCT
EcoO65I 1248 GGTCACC G^GT[ACGT]ACC
HpaI 183 GTTAAC GTT^AAC
KasI 27 GGCGCC G^GCGCC
NarI 27 GGCGCC GG^CGCC
PflFI 1275 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2219 ACCGGT A^CCGGT
PmaCI 2713 CACGTG CAC^GTG
PmlI 2713 CACGTG CAC^GTG
PshAI 1263 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6760 AACGTT AA^CGTT
SbfI 5142 CCTGCAGG CCTGCA^GG
SfiI 2586 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 27 GGCGCC GGC^GCC
SpeI 1863 ACTAGT A^CTAGT
Sse8387I 5142 CCTGCAGG CCTGCA^GG
SwaI 4073 ATTTAAAT ATTT^AAAT
Tth111I 1275 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 797 GGTACC G^GTACC
3305 GGTACC
AccI 1171 GTATAC GT^[AC][GT]AC
8904 GTAGAC
AflIII 4625 ACATGT A^C[GA][CT]GT
5555 ACATGT
Asp718I 797 GGTACC G^GTACC
3305 GGTACC
AviII 3068 TGCGCA TGC^GCA
3875 TGCGCA
BamHI 146 GGATCC G^GATCC
7393 GGATCC
BclI 1295 TGATCA T^GATCA
5171 TGATCA
BsaBI 1833 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2163 GATTCGAATC
BsaI 1261 GAGACC GGTCTC(1/5)
2038 GAGACC
BsmBI 2857 CGTCTC CGTCTC(1/5)
2158 GAGACG
Bsp1407I 264 TGTACA T^GTACA
8323 TGTACA
BspLU11I 4625 ACATGT A^CATGT
5555 ACATGT
BsrGI 264 TGTACA T^GTACA
8323 TGTACA
BstAPI 1181 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4671 GCACTCCCTGC
BstBI 1932 TTCGAA TT^CGAA
2165 TTCGAA
DraIII 7685 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
8103 CACCAGGTG
EcoRV 301 GATATC GAT^ATC
8195 GATATC
EcoT22I 5658 ATGCAT ATGCA^T
8367 ATGCAT
FbaI 1295 TGATCA T^GATCA
5171 TGATCA
FspI 3068 TGCGCA TGC^GCA
3875 TGCGCA
KpnI 797 GGTACC GGTAC^C
3305 GGTACC
KspI 44 CCGCGG CCGC^GG
58 CCGCGG
MamI 1833 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2163 GATTCGAATC
MfeI 257 CAATTG C^AATTG
6914 CAATTG
MunI 257 CAATTG C^AATTG
6914 CAATTG
NaeI 3937 GCCGGC GCC^GGC
7306 GCCGGC
NgoMIV 3937 GCCGGC G^CCGGC
7306 GCCGGC
NsiI 5658 ATGCAT ATGCA^T
8367 ATGCAT
NspV 1932 TTCGAA TT^CGAA
2165 TTCGAA
PaeR7I 3868 CTCGAG C^TCGAG
4201 CTCGAG
SacII 44 CCGCGG CCGC^GG
58 CCGCGG
SapI 1825 GAAGAGC GCTCTTC(1/4)
5160 GCTCTTC
ScaI 6268 AGTACT AGT^ACT
8510 AGTACT
SexAI 4556 ACCTGGT A^CC[AT]GGT
8104 ACCAGGT
SfuI 1932 TTCGAA TT^CGAA
2165 TTCGAA
SspBI 264 TGTACA T^GTACA
8323 TGTACA
XhoI 3868 CTCGAG C^TCGAG
4201 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AatII AccII AccIII AciI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshBI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI