##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvrII BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I CelII CpoI DraIII Eco47III
FseI HpaI KpnI KspI MroI
NdeI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacII SexAI SfuI
SgrAI SpeI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AclI 4760 AACGTT AA^CGTT
AflII 6233 CTTAAG C^TTAAG
AflIII 5864 ACGCGT A^C[GA][CT]GT
AocI 722 CCTCAGG CC^T[ACGT]AGG
AviII 1252 TGCGCA TGC^GCA
BbrPI 6528 CACGTG CAC^GTG
BcgI 3375 CGACCGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6233 CTTAAG C^TTAAG
BsaBI 2305 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1646 ATCGAT AT^CGAT
Bsu36I 722 CCTCAGG CC^T[ACGT]AGG
ClaI 1646 ATCGAT AT^CGAT
EagI 1330 CGGCCG C^GGCCG
EclXI 1330 CGGCCG C^GGCCG
Eco52I 1330 CGGCCG C^GGCCG
Eco81I 722 CCTCAGG CC^T[ACGT]AGG
EcoRI 2351 GAATTC G^AATTC
EcoRV 2546 GATATC GAT^ATC
FspI 1252 TGCGCA TGC^GCA
MamI 2305 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5864 ACGCGT A^CGCGT
NheI 5500 GCTAGC G^CTAGC
PmaCI 6528 CACGTG CAC^GTG
PmlI 6528 CACGTG CAC^GTG
Psp1406I 4760 AACGTT AA^CGTT
SalI 5769 GTCGAC G^TCGAC
SbfI 3382 CCTGCAGG CCTGCA^GG
ScaI 7257 AGTACT AGT^ACT
SfiI 2587 GGCCAGAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 3687 TACGTA TAC^GTA
Sse8387I 3382 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 5769 GTCGAC GT^[AC][GT]AC
6863 GTAGAC
AhdI 1459 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACTTGGAGTC
AspEI 1459 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACTTGGAGTC
BanI 6012 GGCGCC G^G[CT][GA]CC
6903 GGCGCC
BbeI 6012 GGCGCC GGCGC^C
6903 GGCGCC
BciVI 4138 GGATAC GTATCC(6/5)
6260 GTATCC
BsaAI 3687 TACGTA [CT]AC^GT[GA]
6528 CACGTG
BsgI 6020 CTGCAC GTGCAG(16/14)
6586 CTGCAC
BsmBI 3371 GAGACG CGTCTC(1/5)
6089 GAGACG
BsmI 1733 GAATGC GAATGC(1/-1)
6158 GAATGC
Bsp1407I 513 TGTACA T^GTACA
2463 TGTACA
BsrGI 513 TGTACA T^GTACA
2463 TGTACA
DraI 2533 TTTAAA TTT^AAA
6408 TTTAAA
DrdI 140 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3956 GACGCGCAGGTC
Eam1105I 1459 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACTTGGAGTC
KasI 6012 GGCGCC G^GCGCC
6903 GGCGCC
NaeI 6009 GCCGGC GCC^GGC
6900 GCCGGC
NarI 6012 GGCGCC GG^CGCC
6903 GGCGCC
NgoMIV 6009 GCCGGC G^CCGGC
6900 GCCGGC
PflMI 4399 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5545 CCAGCAACTGG
SfoI 6012 GGCGCC GGC^GCC
6903 GGCGCC
SmaI 4650 CCCGGG CCC^GGG
6165 CCCGGG
SphI 582 GCATGC GCATG^C
6486 GCATGC
SspBI 513 TGTACA T^GTACA
2463 TGTACA
StuI 2418 AGGCCT AGG^CCT
2595 AGGCCT
Van91I 4399 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5545 CCAGCAACTGG
XbaI 3847 TCTAGA T^CTAGA
7579 TCTAGA
XmaCI 4650 CCCGGG C^CCGGG
6165 CCCGGG
XmaI 4650 CCCGGG C^CCGGG
6165 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BamHI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PaeR7I PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoI XhoII XmnI