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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BfrI BlnI BseAI
BsiEI BspDI BspEI BssSI ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FspI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NotI
NsiI PacI PaeR7I PinAI PmeI
PshBI PvuI RsrII SacII SalI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SpeI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 712 GTATAC GT^[AC][GT]AC
AclI 5417 AACGTT AA^CGTT
AcyI 344 GACGCC G[GA]^CG[CT]C
AhdI 81 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2501 AGCGCT AGC^GCT
ApaI 3464 GGGCCC GGGCC^C
Asp700I 4879 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 81 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 460 GGATCC G^GATCC
BbrPI 1096 CACGTG CAC^GTG
BcgI 938 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5298 TGATCA T^GATCA
BlpI 2099 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2099 GCTGAGC GC^T[ACGT]AGC
BsaHI 344 GACGCC G[GA]^CG[CT]C
BsiWI 2011 CGTACG C^GTACG
BspHI 4938 TCATGA T^CATGA
BspLU11I 5331 ACATGT A^CATGT
BsrBI 5741 CCGCTC CCGCTC(-3/-3)
BssHII 4301 GCGCGC G^CGCGC
Bst1107I 712 GTATAC GTA^TAC
BstBI 557 TTCGAA TT^CGAA
BstZ17I 712 GTATAC GTA^TAC
CelII 2099 GCTGAGC GC^T[ACGT]AGC
Eam1105I 81 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2501 AGCGCT AGC^GCT
FbaI 5298 TGATCA T^GATCA
FseI 959 GGCCGGCC GGCCGG^CC
Hin1I 344 GACGCC G[GA]^CG[CT]C
HindIII 5781 AAGCTT A^AGCTT
NdeI 4470 CATATG CA^TATG
NheI 3587 GCTAGC G^CTAGC
NruI 5845 TCGCGA TCG^CGA
NspV 557 TTCGAA TT^CGAA
PmaCI 1096 CACGTG CAC^GTG
PmlI 1096 CACGTG CAC^GTG
Psp1406I 5417 AACGTT AA^CGTT
RcaI 4938 TCATGA T^CATGA
SfiI 2952 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 557 TTCGAA TT^CGAA
XmnI 4879 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspI 3211 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4447 GACCCTGTC
AvaI 4736 CCCGAG C^[CT]CG[GA]G
5912 CCCGAG
BciVI 5900 GGATAC GTATCC(6/5)
2445 GTATCC
BsaBI 4809 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4930 GATTAGCATC
BsmBI 3865 CGTCTC CGTCTC(1/5)
3035 GAGACG
BsoBI 4736 CCCGAG C^[CT]CG[GA]G
5912 CCCGAG
Bsp1407I 1092 TGTACA T^GTACA
4489 TGTACA
BsrGI 1092 TGTACA T^GTACA
4489 TGTACA
DraIII 4413 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5533 CACGCAGTG
HaeII 2186 AGCGCC [GA]GCGC^[CT]
2501 AGCGCT
MamI 4809 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4930 GATTAGCATC
PflFI 3211 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4447 GACCCTGTC
PshAI 1405 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5216 GACTTTGGTC
SapI 231 GAAGAGC GCTCTTC(1/4)
4688 GAAGAGC
SbfI 1441 CCTGCAGG CCTGCA^GG
2278 CCTGCAGG
SphI 1044 GCATGC GCATG^C
2764 GCATGC
Sse8387I 1441 CCTGCAGG CCTGCA^GG
2278 CCTGCAGG
SspBI 1092 TGTACA T^GTACA
4489 TGTACA
Tth111I 3211 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4447 GACCCTGTC
XcmI 1149 CCATCCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4998 CCAACATCGTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII