##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AclI AgeI AscI AseI
AsnI AviII BsiEI BsiWI Bsp1407I
BspDI BsrGI BssHII ClaI CpoI
EagI EclXI Eco52I FseI FspI
HpaI KspI MfeI MluI MunI
NdeI NotI NruI PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SfiI SgrAI SnaBI SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 5554 GGTACC G^GTACC
AccIII 420 TCCGGA T^CCGGA
Alw44I 8014 GTGCAC G^TGCAC
ApaLI 8014 GTGCAC G^TGCAC
Asp718I 5554 GGTACC G^GTACC
AspI 8877 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 31 GGCGCC GGCGC^C
BcgI 6505 CGATTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 5409 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 420 TCCGGA T^CCGGA
BsmBI 569 CGTCTC CGTCTC(1/5)
BspEI 420 TCCGGA T^CCGGA
BspLU11I 8790 ACATGT A^CATGT
BssSI 5709 CTCGTG CACGAG(-5/-1)
Bst1107I 5457 GTATAC GTA^TAC
BstBI 717 TTCGAA TT^CGAA
BstEII 5978 GGTCACC G^GT[ACGT]ACC
BstPI 5978 GGTCACC G^GT[ACGT]ACC
BstZ17I 5457 GTATAC GTA^TAC
DraI 8090 TTTAAA TTT^AAA
DrdI 4625 GACACGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5978 GGTCACC G^GT[ACGT]ACC
EcoRV 260 GATATC GAT^ATC
EcoT22I 4536 ATGCAT ATGCA^T
HindIII 428 AAGCTT A^AGCTT
KasI 31 GGCGCC G^GCGCC
KpnI 5554 GGTACC GGTAC^C
MamI 5409 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 420 TCCGGA T^CCGGA
NarI 31 GGCGCC GG^CGCC
NheI 1048 GCTAGC G^CTAGC
NsiI 4536 ATGCAT ATGCA^T
NspV 717 TTCGAA TT^CGAA
PflFI 8877 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 3147 GTCGAC G^TCGAC
SbfI 864 CCTGCAGG CCTGCA^GG
SexAI 7148 ACCAGGT A^CC[AT]GGT
SfoI 31 GGCGCC GGC^GCC
SfuI 717 TTCGAA TT^CGAA
SpeI 2141 ACTAGT A^CTAGT
SphI 6325 GCATGC GCATG^C
Sse8387I 864 CCTGCAGG CCTGCA^GG
SspI 6164 AATATT AAT^ATT
Tth111I 8877 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3147 GTCGAC GT^[AC][GT]AC
5457 GTATAC
AccII 54 CGCG CG^CG
2654 CGCG
AflII 4049 CTTAAG C^TTAAG
7004 CTTAAG
AhdI 562 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3244 GACAGCCAGTC
Aor51HI 2739 AGCGCT AGC^GCT
2867 AGCGCT
AspEI 562 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3244 GACAGCCAGTC
AvrII 7856 CCTAGG C^CTAGG
8656 CCTAGG
BamHI 5093 GGATCC G^GATCC
8652 GGATCC
BciVI 8506 GGATAC GTATCC(6/5)
6488 GTATCC
BfrI 4049 CTTAAG C^TTAAG
7004 CTTAAG
BlnI 7856 CCTAGG C^CTAGG
8656 CCTAGG
BsrFI 5643 GCCGGC [GA]^CCGG[CT]
6104 GCCGGC
BstUI 54 CGCG CG^CG
2654 CGCG
Cfr10I 5643 GCCGGC [GA]^CCGG[CT]
6104 GCCGGC
Eam1105I 562 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3244 GACAGCCAGTC
Eco47III 2739 AGCGCT AGC^GCT
2867 AGCGCT
EcoRI 1208 GAATTC G^AATTC
7485 GAATTC
HincII 1803 GTCAAC GT[CT]^[GA]AC
3147 GTCGAC
HindII 1803 GTCAAC GT[CT]^[GA]AC
3147 GTCGAC
MvnI 54 CGCG CG^CG
2654 CGCG
NaeI 5643 GCCGGC GCC^GGC
6104 GCCGGC
NcoI 3039 CCATGG C^CATGG
7723 CCATGG
NgoMIV 5643 GCCGGC G^CCGGC
6104 GCCGGC
PaeR7I 485 CTCGAG C^TCGAG
1130 CTCGAG
ScaI 6264 AGTACT AGT^ACT
6834 AGTACT
StuI 861 AGGCCT AGG^CCT
4227 AGGCCT
XhoI 485 CTCGAG C^TCGAG
1130 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI