##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BbrPI BbvCI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I CelII ClaI DraIII
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRV FbaI FokI FseI HgaI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI Tth111I Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 983 GTAGAC GT^[AC][GT]AC
AcyI 323 GGCGCC G[GA]^CG[CT]C
AfaI 1286 GTAC GT^AC
AflII 739 CTTAAG C^TTAAG
AflIII 1350 ACGTGT A^C[GA][CT]GT
Aor51HI 673 AGCGCT AGC^GCT
ApaI 200 GGGCCC GGGCC^C
AspHI 558 GTGCTC G[AT]GC[AT]^C
AvaI 780 CCCGGG C^[CT]CG[GA]G
AviII 478 TGCGCA TGC^GCA
BamHI 965 GGATCC G^GATCC
BbeI 323 GGCGCC GGCGC^C
BfrI 739 CTTAAG C^TTAAG
BglI 93 GCCGCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 304 CTCCAG CTGGAG(16/14)
BsaHI 323 GGCGCC G[GA]^CG[CT]C
BseRI 264 CTCCTC GAGGAG(10/8)
BsiHKAI 558 GTGCTC G[AT]GC[AT]^C
BsmFI 778 GTCCC GGGAC(10/14)
BsoBI 780 CCCGGG C^[CT]CG[GA]G
BspHI 710 TCATGA T^CATGA
BspMI 760 ACCTGC ACCTGC(4/8)
BsrBI 344 GAGCGG CCGCTC(-3/-3)
BssHII 125 GCGCGC G^CGCGC
BstXI 1108 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 503 CGGACCG CG^G[AT]CCG
DrdI 892 GACCAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 673 AGCGCT AGC^GCT
EcoRI 861 GAATTC G^AATTC
EcoT22I 1454 ATGCAT ATGCA^T
FspI 478 TGCGCA TGC^GCA
Hin1I 323 GGCGCC G[GA]^CG[CT]C
KasI 323 GGCGCC G^GCGCC
MslI 1457 CATGTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 454 GCCGGC GCC^GGC
NarI 323 GGCGCC GG^CGCC
NgoMIV 454 GCCGGC G^CCGGC
NheI 1003 GCTAGC G^CTAGC
NsiI 1454 ATGCAT ATGCA^T
PleI 1104 GACTC GAGTC(4/5)
PstI 113 CTGCAG CTGCA^G
RcaI 710 TCATGA T^CATGA
RsaI 1286 GTAC GT^AC
RsrII 503 CGGACCG CG^G[AT]CCG
SfoI 323 GGCGCC GGC^GCC
SmaI 780 CCCGGG CCC^GGG
SspI 383 AATATT AAT^ATT
SwaI 1663 ATTTAAAT ATTT^AAAT
TaqI 688 TCGA T^CGA
TthHB8I 688 TCGA T^CGA
XmaCI 780 CCCGGG C^CCGGG
XmaI 780 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 60 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1558 CAGCTACTG
AvaII 504 GGACC G^G[AT]CC
777 GGTCC
BalI 852 TGGCCA TGG^CCA
1274 TGGCCA
BanI 323 GGCGCC G^G[CT][GA]CC
548 GGTGCC
BanII 107 GGGCTC G[GA]GC[CT]^C
200 GGGCCC
BbsI 151 GAAGAC GAAGAC(2/6)
976 GTCTTC
BfaI 1004 CTAG C^TAG
1651 CTAG
BpuAI 151 GAAGAC GAAGAC(2/6)
976 GTCTTC
BsiEI 178 CGGCCG CG[GA][CT]^CG
452 CGGCCG
BsmI 927 GAATGC GAATGC(1/-1)
1229 GCATTC
BsrFI 454 GCCGGC [GA]^CCGG[CT]
545 GCCGGT
BsrI 1272 ACTGG ACTGG(1/-1)
1010 CCAGT
BstYI 965 GGATCC [GA]^GATC[CT]
1151 AGATCC
Cfr10I 454 GCCGGC [GA]^CCGG[CT]
545 GCCGGT
DraI 231 TTTAAA TTT^AAA
1664 TTTAAA
DraII 199 GGGGCCC [GA]G^G[ACGT]CC[CT]
200 GGGCCCC
EagI 178 CGGCCG C^GGCCG
452 CGGCCG
EclXI 178 CGGCCG C^GGCCG
452 CGGCCG
Eco52I 178 CGGCCG C^GGCCG
452 CGGCCG
EcoO109I 199 GGGGCCC [GA]G^G[ACGT]CC[CT]
200 GGGCCCC
HaeII 323 GGCGCC [GA]GCGC^[CT]
673 AGCGCT
MaeI 1004 CTAG C^TAG
1651 CTAG
MaeII 595 ACGT A^CGT
1350 ACGT
MflI 965 GGATCC [GA]^GATC[CT]
1151 AGATCC
MluNI 852 TGGCCA TGG^CCA
1274 TGGCCA
MscI 852 TGGCCA TGG^CCA
1274 TGGCCA
NspI 751 ACATGC [GA]CATG^[CT]
1456 GCATGT
PvuII 116 CAGCTG CAG^CTG
577 CAGCTG
SmlI 553 CTCAAG C^T[CT][GA]AG
739 CTTAAG
Tsp45I 448 GTGAC ^GT[GC]AC
991 GTCAC
TspRI 1011 CAGTG CA[GC]TG[ACGT][ACGT]^
1271 CACTG
XhoII 965 GGATCC [GA]^GATC[CT]
1151 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BssKI BstAPI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfcI StyI TfiI Tru9I
TseI Tsp509I