##################################
# NO CUT ENZYMES ( 28 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
AvrII BlnI BsiWI BssHII Bst1107I
BstZ17I FseI KspI MluI PacI
PinAI PmeI PshBI Psp1406I SacII
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 16 enzymes) #
###################################
AatII 423 GACGTC GACGT^C
Acc65I 3172 GGTACC G^GTACC
AflII 2361 CTTAAG C^TTAAG
Asp718I 3172 GGTACC G^GTACC
BfrI 2361 CTTAAG C^TTAAG
BstBI 14034 TTCGAA TT^CGAA
CpoI 67 CGGTCCG CG^G[AT]CCG
EcoT22I 1368 ATGCAT ATGCA^T
KpnI 3172 GGTACC GGTAC^C
NotI 120 GCGGCCGC GC^GGCCGC
NruI 1967 TCGCGA TCG^CGA
NsiI 1368 ATGCAT ATGCA^T
NspV 14034 TTCGAA TT^CGAA
PvuI 12158 CGATCG CGAT^CG
RsrII 67 CGGTCCG CG^G[AT]CCG
SfuI 14034 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 11038 GTGCAC G^TGCAC
14093 GTGCAC
AocI 1613 CCTGAGG CC^T[ACGT]AGG
13713 CCTGAGG
ApaI 6834 GGGCCC GGGCC^C
8381 GGGCCC
ApaLI 11038 GTGCAC G^TGCAC
14093 GTGCAC
BspDI 4191 ATCGAT AT^CGAT
7767 ATCGAT
BspHI 3041 TCATGA T^CATGA
4411 TCATGA
BssSI 9303 CACGAG CACGAG(-5/-1)
9726 CACGAG
Bsu36I 1613 CCTGAGG CC^T[ACGT]AGG
13713 CCTGAGG
ClaI 4191 ATCGAT AT^CGAT
7767 ATCGAT
Eco81I 1613 CCTGAGG CC^T[ACGT]AGG
13713 CCTGAGG
HpaI 909 GTTAAC GTT^AAC
2373 GTTAAC
NdeI 2656 CATATG CA^TATG
6739 CATATG
NheI 58 GCTAGC G^CTAGC
9644 GCTAGC
PaeR7I 6894 CTCGAG C^TCGAG
11779 CTCGAG
RcaI 3041 TCATGA T^CATGA
4411 TCATGA
SalI 9798 GTCGAC G^TCGAC
13026 GTCGAC
XhoI 6894 CTCGAG C^TCGAG
11779 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 197 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AhdI AluI
AlwI AlwNI Aor51HI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
AviII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI SphI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI