##################################
# NO CUT ENZYMES ( 32 enzymes) #
##################################
Acc65I AgeI AocI AscI Asp718I
AviII AvrII BlnI BspHI BspLU11I
BstBI Bsu36I CpoI Eco81I EcoRV
FspI KpnI NdeI NruI NspV
PacI PaeR7I PinAI PmeI RcaI
RsrII SalI SfiI SfuI SpeI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 3376 AACGTT AA^CGTT
AflII 8706 CTTAAG C^TTAAG
Asp700I 10613 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 11682 GGATCC G^GATCC
BbrPI 10830 CACGTG CAC^GTG
BfrI 8706 CTTAAG C^TTAAG
BsaBI 3035 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 6984 CGTACG C^GTACG
BspDI 5484 ATCGAT AT^CGAT
BssHII 8668 GCGCGC G^CGCGC
Bst1107I 10515 GTATAC GTA^TAC
BstZ17I 10515 GTATAC GTA^TAC
ClaI 5484 ATCGAT AT^CGAT
EcoRI 10950 GAATTC G^AATTC
EcoT22I 9287 ATGCAT ATGCA^T
FseI 746 GGCCGGCC GGCCGG^CC
MamI 3035 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 5198 GCGGCCGC GC^GGCCGC
NsiI 9287 ATGCAT ATGCA^T
PmaCI 10830 CACGTG CAC^GTG
PmlI 10830 CACGTG CAC^GTG
PshAI 6377 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3376 AACGTT AA^CGTT
PvuI 8488 CGATCG CGAT^CG
SbfI 6263 CCTGCAGG CCTGCA^GG
ScaI 9430 AGTACT AGT^ACT
SgrAI 4525 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 11356 TACGTA TAC^GTA
SphI 7525 GCATGC GCATG^C
Sse8387I 6263 CCTGCAGG CCTGCA^GG
XbaI 11313 TCTAGA T^CTAGA
XmnI 10613 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 6354 GTCTAC GT^[AC][GT]AC
10515 GTATAC
AccIII 1079 TCCGGA T^CCGGA
4439 TCCGGA
AflIII 7135 ACGCGT A^C[GA][CT]GT
11437 ACGCGT
Aor51HI 324 AGCGCT AGC^GCT
11096 AGCGCT
AseI 10748 ATTAAT AT^TAAT
11104 ATTAAT
AsnI 10748 ATTAAT AT^TAAT
11104 ATTAAT
BbeI 2871 GGCGCC GGCGC^C
4716 GGCGCC
BcgI 5486 CGATGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
141 GCAAGCCCGTCG
BseAI 1079 TCCGGA T^CCGGA
4439 TCCGGA
BsmBI 1389 GAGACG CGTCTC(1/5)
4521 GAGACG
Bsp1407I 5212 TGTACA T^GTACA
7033 TGTACA
BspEI 1079 TCCGGA T^CCGGA
4439 TCCGGA
BsrGI 5212 TGTACA T^GTACA
7033 TGTACA
BstEII 1470 GGTCACC G^GT[ACGT]ACC
8984 GGTGACC
BstPI 1470 GGTCACC G^GT[ACGT]ACC
8984 GGTGACC
DraIII 570 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3786 CACAGGGTG
DrdI 8987 GACCACGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11076 GACATAACTGTC
Eco47III 324 AGCGCT AGC^GCT
11096 AGCGCT
EcoO65I 1470 GGTCACC G^GT[ACGT]ACC
8984 GGTGACC
HpaI 6876 GTTAAC GTT^AAC
11760 GTTAAC
KasI 2871 GGCGCC G^GCGCC
4716 GGCGCC
MfeI 2761 CAATTG C^AATTG
6136 CAATTG
MluI 7135 ACGCGT A^CGCGT
11437 ACGCGT
MroI 1079 TCCGGA T^CCGGA
4439 TCCGGA
MunI 2761 CAATTG C^AATTG
6136 CAATTG
NarI 2871 GGCGCC GG^CGCC
4716 GGCGCC
NheI 2715 GCTAGC G^CTAGC
6107 GCTAGC
PshBI 10748 ATTAAT AT^TAAT
11104 ATTAAT
SexAI 2233 ACCAGGT A^CC[AT]GGT
7732 ACCTGGT
SfoI 2871 GGCGCC GGC^GCC
4716 GGCGCC
SspBI 5212 TGTACA T^GTACA
7033 TGTACA
XcmI 703 CCATCCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
8296 CCAACCTCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AhdI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmaCI XmaI