##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AclI Aor51HI AscI AseI
AsnI Asp718I BamHI BsiWI BstBI
CpoI DrdI Eco47III EcoRI EcoRV
HpaI KpnI KspI MfeI MluI
MunI NheI NotI NruI NspV
PacI PaeR7I PmeI PshBI Psp1406I
PvuI RsrII SacI SacII SbfI
SfiI SfuI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 4818 CTTAAG C^TTAAG
AgeI 3121 ACCGGT A^CCGGT
AhdI 3736 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 112 GGGCCC GGGCC^C
Asp700I 714 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3736 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1094 TGCGCA TGC^GCA
AvrII 768 CCTAGG C^CTAGG
BalI 4100 TGGCCA TGG^CCA
BbeI 85 GGCGCC GGCGC^C
BcgI 5671 CGATTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2173 GTATCC GTATCC(6/5)
BfrI 4818 CTTAAG C^TTAAG
BlnI 768 CCTAGG C^CTAGG
BsaBI 3103 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 5506 GAGACG CGTCTC(1/5)
BspDI 1059 ATCGAT AT^CGAT
BspHI 4236 TCATGA T^CATGA
BssHII 1164 GCGCGC G^CGCGC
BstAPI 3855 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2928 GGTTACC G^GT[ACGT]ACC
BstPI 2928 GGTTACC G^GT[ACGT]ACC
ClaI 1059 ATCGAT AT^CGAT
Eam1105I 3736 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2928 GGTTACC G^GT[ACGT]ACC
EcoT22I 1921 ATGCAT ATGCA^T
FseI 3867 GGCCGGCC GGCCGG^CC
FspI 1094 TGCGCA TGC^GCA
KasI 85 GGCGCC G^GCGCC
MamI 3103 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4100 TGGCCA TGG^CCA
MscI 4100 TGGCCA TGG^CCA
NarI 85 GGCGCC GG^CGCC
NsiI 1921 ATGCAT ATGCA^T
PinAI 3121 ACCGGT A^CCGGT
RcaI 4236 TCATGA T^CATGA
SalI 3526 GTCGAC G^TCGAC
ScaI 5188 AGTACT AGT^ACT
SexAI 1227 ACCTGGT A^CC[AT]GGT
SfoI 85 GGCGCC GGC^GCC
SgrAI 203 CGCCGGTG C[GA]^CCGG[CT]G
SspI 839 AATATT AAT^ATT
StuI 3834 AGGCCT AGG^CCT
XmnI 714 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 2124 GACGTC GACGT^C
3658 GACGTC
AccIII 55 TCCGGA T^CCGGA
2738 TCCGGA
Alw44I 1119 GTGCAC G^TGCAC
3996 GTGCAC
ApaLI 1119 GTGCAC G^TGCAC
3996 GTGCAC
AspI 2755 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3928 GACCACGTC
BclI 3431 TGATCA T^GATCA
4720 TGATCA
BlpI 1459 GCTGAGC GC^T[ACGT]AGC
2643 GCTTAGC
Bpu1102I 1459 GCTGAGC GC^T[ACGT]AGC
2643 GCTTAGC
BsaI 2155 GAGACC GGTCTC(1/5)
4593 GAGACC
BseAI 55 TCCGGA T^CCGGA
2738 TCCGGA
BsiEI 72 CGGCCG CG[GA][CT]^CG
3866 CGGCCG
BspEI 55 TCCGGA T^CCGGA
2738 TCCGGA
BspLU11I 4776 ACATGT A^CATGT
6272 ACATGT
BsrBI 89 CCGCTC CCGCTC(-3/-3)
3628 CCGCTC
CelII 1459 GCTGAGC GC^T[ACGT]AGC
2643 GCTTAGC
EagI 72 CGGCCG C^GGCCG
3866 CGGCCG
EclXI 72 CGGCCG C^GGCCG
3866 CGGCCG
Eco52I 72 CGGCCG C^GGCCG
3866 CGGCCG
FbaI 3431 TGATCA T^GATCA
4720 TGATCA
HindIII 1532 AAGCTT A^AGCTT
3508 AAGCTT
MroI 55 TCCGGA T^CCGGA
2738 TCCGGA
NdeI 4686 CATATG CA^TATG
4991 CATATG
PflFI 2755 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3928 GACCACGTC
PflMI 1326 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3930 CCACGTCCTGG
SpeI 3144 ACTAGT A^CTAGT
5592 ACTAGT
Tth111I 2755 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3928 GACCACGTC
Van91I 1326 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3930 CCACGTCCTGG
XbaI 2310 TCTAGA T^CTAGA
4458 TCTAGA
XcmI 1000 CCAGTGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4042 CCAGACCTTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
Bst1107I BstF5I BstNI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII