##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AocI AscI AseI AsnI
Asp718I BamHI BbrPI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
Eco81I EcoRV FseI KpnI KspI
MamI MfeI MluI MroI MunI
NheI NruI NspV PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI ScaI
SfuI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 1281 CTTAAG C^TTAAG
Alw44I 6455 GTGCAC G^TGCAC
ApaLI 6455 GTGCAC G^TGCAC
AspI 1838 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5114 TGCGCA TGC^GCA
AvrII 5238 CCTAGG C^CTAGG
BbeI 2171 GGCGCC GGCGC^C
BclI 643 TGATCA T^GATCA
BfrI 1281 CTTAAG C^TTAAG
BglII 242 AGATCT A^GATCT
BlnI 5238 CCTAGG C^CTAGG
Bsp1407I 4962 TGTACA T^GTACA
BsrGI 4962 TGTACA T^GTACA
DraIII 2337 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 402 GACGGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 643 TGATCA T^GATCA
FspI 5114 TGCGCA TGC^GCA
HindIII 4222 AAGCTT A^AGCTT
KasI 2171 GGCGCC G^GCGCC
NaeI 2168 GCCGGC GCC^GGC
NarI 2171 GGCGCC GG^CGCC
NdeI 5072 CATATG CA^TATG
NgoMIV 2168 GCCGGC G^CCGGC
NotI 1778 GCGGCCGC GC^GGCCGC
PacI 4648 TTAATTAA TTAAT^TAA
PaeR7I 6417 CTCGAG C^TCGAG
PflFI 1838 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 2338 ACCAGGT A^CC[AT]GGT
SfoI 2171 GGCGCC GGC^GCC
SgrAI 2434 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3365 CCCGGG CCC^GGG
SspBI 4962 TGTACA T^GTACA
SspI 6606 AATATT AAT^ATT
Tth111I 1838 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 6417 CTCGAG C^TCGAG
XmaCI 3365 CCCGGG C^CCGGG
XmaI 3365 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 3638 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5620 GACACAAAGTC
AspEI 3638 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5620 GACACAAAGTC
BcgI 3005 CGACACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2897 GCATGAAGGTCG
BciVI 3460 GTATCC GTATCC(6/5)
4107 GTATCC
BstEII 1589 GGTCACC G^GT[ACGT]ACC
5257 GGTGACC
BstPI 1589 GGTCACC G^GT[ACGT]ACC
5257 GGTGACC
DraI 4084 TTTAAA TTT^AAA
4410 TTTAAA
Eam1105I 3638 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5620 GACACAAAGTC
EcoO65I 1589 GGTCACC G^GT[ACGT]ACC
5257 GGTGACC
EcoRI 792 GAATTC G^AATTC
4579 GAATTC
EcoT22I 3819 ATGCAT ATGCA^T
3888 ATGCAT
HpaI 315 GTTAAC GTT^AAC
3974 GTTAAC
NcoI 3289 CCATGG C^CATGG
3853 CCATGG
NsiI 3819 ATGCAT ATGCA^T
3888 ATGCAT
PshAI 778 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2286 GACCCCCGTC
SbfI 175 CCTGCAGG CCTGCA^GG
1919 CCTGCAGG
SfiI 1691 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1899 GGCCCACCCGGCC
SphI 3821 GCATGC GCATG^C
5348 GCATGC
Sse8387I 175 CCTGCAGG CCTGCA^GG
1919 CCTGCAGG
XbaI 4950 TCTAGA T^CTAGA
5682 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI Aor51HI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SalI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI