################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AocI AscI AseI AsnI Asp718I BamHI BbrPI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI Eco81I EcoRV FseI KpnI KspI MamI MfeI MluI MroI MunI NheI NruI NspV PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SapI ScaI SfuI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflII 1281 CTTAAG C^TTAAG Alw44I 6455 GTGCAC G^TGCAC ApaLI 6455 GTGCAC G^TGCAC AspI 1838 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5114 TGCGCA TGC^GCA AvrII 5238 CCTAGG C^CTAGG BbeI 2171 GGCGCC GGCGC^C BclI 643 TGATCA T^GATCA BfrI 1281 CTTAAG C^TTAAG BglII 242 AGATCT A^GATCT BlnI 5238 CCTAGG C^CTAGG Bsp1407I 4962 TGTACA T^GTACA BsrGI 4962 TGTACA T^GTACA DraIII 2337 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 402 GACGGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 643 TGATCA T^GATCA FspI 5114 TGCGCA TGC^GCA HindIII 4222 AAGCTT A^AGCTT KasI 2171 GGCGCC G^GCGCC NaeI 2168 GCCGGC GCC^GGC NarI 2171 GGCGCC GG^CGCC NdeI 5072 CATATG CA^TATG NgoMIV 2168 GCCGGC G^CCGGC NotI 1778 GCGGCCGC GC^GGCCGC PacI 4648 TTAATTAA TTAAT^TAA PaeR7I 6417 CTCGAG C^TCGAG PflFI 1838 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC SexAI 2338 ACCAGGT A^CC[AT]GGT SfoI 2171 GGCGCC GGC^GCC SgrAI 2434 CACCGGCG C[GA]^CCGG[CT]G SmaI 3365 CCCGGG CCC^GGG SspBI 4962 TGTACA T^GTACA SspI 6606 AATATT AAT^ATT Tth111I 1838 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 6417 CTCGAG C^TCGAG XmaCI 3365 CCCGGG C^CCGGG XmaI 3365 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 3638 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5620 GACACAAAGTC AspEI 3638 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5620 GACACAAAGTC BcgI 3005 CGACACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2897 GCATGAAGGTCG BciVI 3460 GTATCC GTATCC(6/5) 4107 GTATCC BstEII 1589 GGTCACC G^GT[ACGT]ACC 5257 GGTGACC BstPI 1589 GGTCACC G^GT[ACGT]ACC 5257 GGTGACC DraI 4084 TTTAAA TTT^AAA 4410 TTTAAA Eam1105I 3638 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5620 GACACAAAGTC EcoO65I 1589 GGTCACC G^GT[ACGT]ACC 5257 GGTGACC EcoRI 792 GAATTC G^AATTC 4579 GAATTC EcoT22I 3819 ATGCAT ATGCA^T 3888 ATGCAT HpaI 315 GTTAAC GTT^AAC 3974 GTTAAC NcoI 3289 CCATGG C^CATGG 3853 CCATGG NsiI 3819 ATGCAT ATGCA^T 3888 ATGCAT PshAI 778 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2286 GACCCCCGTC SbfI 175 CCTGCAGG CCTGCA^GG 1919 CCTGCAGG SfiI 1691 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1899 GGCCCACCCGGCC SphI 3821 GCATGC GCATG^C 5348 GCATGC Sse8387I 175 CCTGCAGG CCTGCA^GG 1919 CCTGCAGG XbaI 4950 TCTAGA T^CTAGA 5682 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SalI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI