################################## # NO CUT ENZYMES ( 28 enzymes) # ################################## AflII AgeI AscI AseI AsnI BfrI BsiWI BssHII Bst1107I BstZ17I CpoI EcoRV FseI MluI NotI NruI PacI PinAI PmeI PshAI PshBI PvuI RsrII SalI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 3996 GGTACC G^GTACC AccIII 2103 TCCGGA T^CCGGA AclI 7934 AACGTT AA^CGTT Aor51HI 11919 AGCGCT AGC^GCT Asp718I 3996 GGTACC G^GTACC BbeI 4689 GGCGCC GGCGC^C BseAI 2103 TCCGGA T^CCGGA BsiEI 13909 CGGCCG CG[GA][CT]^CG BspDI 11816 ATCGAT AT^CGAT BspEI 2103 TCCGGA T^CCGGA BstBI 5775 TTCGAA TT^CGAA ClaI 11816 ATCGAT AT^CGAT DraI 11738 TTTAAA TTT^AAA DrdI 1286 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 13909 CGGCCG C^GGCCG EclXI 13909 CGGCCG C^GGCCG Eco47III 11919 AGCGCT AGC^GCT Eco52I 13909 CGGCCG C^GGCCG HpaI 4786 GTTAAC GTT^AAC KasI 4689 GGCGCC G^GCGCC KpnI 3996 GGTACC GGTAC^C KspI 14603 CCGCGG CCGC^GG MroI 2103 TCCGGA T^CCGGA NaeI 1443 GCCGGC GCC^GGC NarI 4689 GGCGCC GG^CGCC NgoMIV 1443 GCCGGC G^CCGGC NheI 2171 GCTAGC G^CTAGC NspV 5775 TTCGAA TT^CGAA Psp1406I 7934 AACGTT AA^CGTT SacII 14603 CCGCGG CCGC^GG SexAI 4993 ACCTGGT A^CC[AT]GGT SfiI 3592 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4689 GGCGCC GGC^GCC SfuI 5775 TTCGAA TT^CGAA SmaI 13329 CCCGGG CCC^GGG XmaCI 13329 CCCGGG C^CCGGG XmaI 13329 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AatII 1292 GACGTC GACGT^C 1829 GACGTC AhdI 1130 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 13737 GACCTCATGTC AspEI 1130 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 13737 GACCTCATGTC AviII 5685 TGCGCA TGC^GCA 11545 TGCGCA AvrII 1996 CCTAGG C^CTAGG 14753 CCTAGG BlnI 1996 CCTAGG C^CTAGG 14753 CCTAGG BsmBI 423 CGTCTC CGTCTC(1/5) 11822 GAGACG BspLU11I 7102 ACATGT A^CATGT 14280 ACATGT Eam1105I 1130 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 13737 GACCTCATGTC FspI 5685 TGCGCA TGC^GCA 11545 TGCGCA HindIII 1844 AAGCTT A^AGCTT 4452 AAGCTT NdeI 1013 CATATG CA^TATG 8185 CATATG PaeR7I 10764 CTCGAG C^TCGAG 13659 CTCGAG SbfI 3358 CCTGCAGG CCTGCA^GG 6151 CCTGCAGG ScaI 1572 AGTACT AGT^ACT 3114 AGTACT Sse8387I 3358 CCTGCAGG CCTGCA^GG 6151 CCTGCAGG XbaI 2182 TCTAGA T^CTAGA 12673 TCTAGA XhoI 10764 CTCGAG C^TCGAG 13659 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 175 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI