##################################
# NO CUT ENZYMES ( 28 enzymes) #
##################################
AflII AgeI AscI AseI AsnI
BfrI BsiWI BssHII Bst1107I BstZ17I
CpoI EcoRV FseI MluI NotI
NruI PacI PinAI PmeI PshAI
PshBI PvuI RsrII SalI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3996 GGTACC G^GTACC
AccIII 2103 TCCGGA T^CCGGA
AclI 7934 AACGTT AA^CGTT
Aor51HI 11919 AGCGCT AGC^GCT
Asp718I 3996 GGTACC G^GTACC
BbeI 4689 GGCGCC GGCGC^C
BseAI 2103 TCCGGA T^CCGGA
BsiEI 13909 CGGCCG CG[GA][CT]^CG
BspDI 11816 ATCGAT AT^CGAT
BspEI 2103 TCCGGA T^CCGGA
BstBI 5775 TTCGAA TT^CGAA
ClaI 11816 ATCGAT AT^CGAT
DraI 11738 TTTAAA TTT^AAA
DrdI 1286 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 13909 CGGCCG C^GGCCG
EclXI 13909 CGGCCG C^GGCCG
Eco47III 11919 AGCGCT AGC^GCT
Eco52I 13909 CGGCCG C^GGCCG
HpaI 4786 GTTAAC GTT^AAC
KasI 4689 GGCGCC G^GCGCC
KpnI 3996 GGTACC GGTAC^C
KspI 14603 CCGCGG CCGC^GG
MroI 2103 TCCGGA T^CCGGA
NaeI 1443 GCCGGC GCC^GGC
NarI 4689 GGCGCC GG^CGCC
NgoMIV 1443 GCCGGC G^CCGGC
NheI 2171 GCTAGC G^CTAGC
NspV 5775 TTCGAA TT^CGAA
Psp1406I 7934 AACGTT AA^CGTT
SacII 14603 CCGCGG CCGC^GG
SexAI 4993 ACCTGGT A^CC[AT]GGT
SfiI 3592 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4689 GGCGCC GGC^GCC
SfuI 5775 TTCGAA TT^CGAA
SmaI 13329 CCCGGG CCC^GGG
XmaCI 13329 CCCGGG C^CCGGG
XmaI 13329 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 1292 GACGTC GACGT^C
1829 GACGTC
AhdI 1130 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
13737 GACCTCATGTC
AspEI 1130 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
13737 GACCTCATGTC
AviII 5685 TGCGCA TGC^GCA
11545 TGCGCA
AvrII 1996 CCTAGG C^CTAGG
14753 CCTAGG
BlnI 1996 CCTAGG C^CTAGG
14753 CCTAGG
BsmBI 423 CGTCTC CGTCTC(1/5)
11822 GAGACG
BspLU11I 7102 ACATGT A^CATGT
14280 ACATGT
Eam1105I 1130 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
13737 GACCTCATGTC
FspI 5685 TGCGCA TGC^GCA
11545 TGCGCA
HindIII 1844 AAGCTT A^AGCTT
4452 AAGCTT
NdeI 1013 CATATG CA^TATG
8185 CATATG
PaeR7I 10764 CTCGAG C^TCGAG
13659 CTCGAG
SbfI 3358 CCTGCAGG CCTGCA^GG
6151 CCTGCAGG
ScaI 1572 AGTACT AGT^ACT
3114 AGTACT
Sse8387I 3358 CCTGCAGG CCTGCA^GG
6151 CCTGCAGG
XbaI 2182 TCTAGA T^CTAGA
12673 TCTAGA
XhoI 10764 CTCGAG C^TCGAG
13659 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 175 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI