##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbeI BbrPI
BcgI BlnI BsaAI BsaBI BseAI
BsiWI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I CpoI DrdI EagI
EclXI Eco52I EcoRI EcoT22I FseI
FspI HpaI KasI KpnI MaeII
MamI MfeI MluI MroI MunI
NarI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Aor51HI 5296 AGCGCT AGC^GCT
BalI 4405 TGGCCA TGG^CCA
BciVI 5517 GGATAC GTATCC(6/5)
BclI 5719 TGATCA T^GATCA
BglII 2683 AGATCT A^GATCT
BsiEI 3282 CGACCG CG[GA][CT]^CG
BspDI 372 ATCGAT AT^CGAT
BstAPI 5243 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3636 GGTGACC G^GT[ACGT]ACC
BstPI 3636 GGTGACC G^GT[ACGT]ACC
BstXI 1589 CCAGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 372 ATCGAT AT^CGAT
DraIII 3140 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 5296 AGCGCT AGC^GCT
EcoO65I 3636 GGTGACC G^GT[ACGT]ACC
EcoRV 4928 GATATC GAT^ATC
FbaI 5719 TGATCA T^GATCA
HincII 42 GTTGAC GT[CT]^[GA]AC
HindII 42 GTTGAC GT[CT]^[GA]AC
KspI 3641 CCGCGG CCGC^GG
MluNI 4405 TGGCCA TGG^CCA
MscI 4405 TGGCCA TGG^CCA
NaeI 386 GCCGGC GCC^GGC
NdeI 6377 CATATG CA^TATG
NgoMIV 386 GCCGGC G^CCGGC
NheI 5670 GCTAGC G^CTAGC
PshAI 2057 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3641 CCGCGG CCGC^GG
SbfI 5680 CCTGCAGG CCTGCA^GG
ScaI 362 AGTACT AGT^ACT
SpeI 6207 ACTAGT A^CTAGT
SphI 5354 GCATGC GCATG^C
Sse8387I 5680 CCTGCAGG CCTGCA^GG
XbaI 5488 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3990 GTCTAC GT^[AC][GT]AC
6151 GTCTAC
AcyI 3354 GACGCC G[GA]^CG[CT]C
4360 GGCGTC
AflII 5712 CTTAAG C^TTAAG
5894 CTTAAG
AflIII 446 ACATGT A^C[GA][CT]GT
2602 ACATGT
AhdI 1689 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4716 GACCAGGGGTC
Alw44I 885 GTGCAC G^TGCAC
4843 GTGCAC
AlwI 5081 GGATC GGATC(4/5)
5554 GGATC
AocI 2259 CCTCAGG CC^T[ACGT]AGG
4917 CCTAAGG
ApaLI 885 GTGCAC G^TGCAC
4843 GTGCAC
AspEI 1689 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4716 GACCAGGGGTC
AspI 1839 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3495 GACGGTGTC
BfrI 5712 CTTAAG C^TTAAG
5894 CTTAAG
BglI 1468 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5027 GCCTTCAGGGC
BsaHI 3354 GACGCC G[GA]^CG[CT]C
4360 GGCGTC
BsaWI 455 ACCGGA [AT]^CCGG[AT]
3523 TCCGGT
BspLU11I 446 ACATGT A^CATGT
2602 ACATGT
BsrDI 17 CATTGC GCAATG(2/0)
5934 CATTGC
BssSI 3430 CACGAG CACGAG(-5/-1)
3893 CTCGTG
Bsu36I 2259 CCTCAGG CC^T[ACGT]AGG
4917 CCTAAGG
DraI 2933 TTTAAA TTT^AAA
5974 TTTAAA
Eam1105I 1689 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4716 GACCAGGGGTC
Eco81I 2259 CCTCAGG CC^T[ACGT]AGG
4917 CCTAAGG
HaeII 3567 AGCGCC [GA]GCGC^[CT]
5296 AGCGCT
Hin1I 3354 GACGCC G[GA]^CG[CT]C
4360 GGCGTC
HindIII 4824 AAGCTT A^AGCTT
5920 AAGCTT
PflFI 1839 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3495 GACGGTGTC
Tth111I 1839 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3495 GACGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI