##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BlnI        BsaAI       BsaBI       BseAI       
BsiWI       BspEI       BspHI       BssHII      Bst1107I    
BstBI       BstZ17I     CpoI        DrdI        EagI        
EclXI       Eco52I      EcoRI       EcoT22I     FseI        
FspI        HpaI        KasI        KpnI        MaeII       
MamI        MfeI        MluI        MroI        MunI        
NarI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SspI        StuI        SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Aor51HI      5296  AGCGCT               AGC^GCT
BalI         4405  TGGCCA               TGG^CCA
BciVI        5517  GGATAC               GTATCC(6/5)
BclI         5719  TGATCA               T^GATCA
BglII        2683  AGATCT               A^GATCT
BsiEI        3282  CGACCG               CG[GA][CT]^CG
BspDI         372  ATCGAT               AT^CGAT
BstAPI       5243  GCACATCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3636  GGTGACC              G^GT[ACGT]ACC
BstPI        3636  GGTGACC              G^GT[ACGT]ACC
BstXI        1589  CCAGTGTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          372  ATCGAT               AT^CGAT
DraIII       3140  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     5296  AGCGCT               AGC^GCT
EcoO65I      3636  GGTGACC              G^GT[ACGT]ACC
EcoRV        4928  GATATC               GAT^ATC
FbaI         5719  TGATCA               T^GATCA
HincII         42  GTTGAC               GT[CT]^[GA]AC
HindII         42  GTTGAC               GT[CT]^[GA]AC
KspI         3641  CCGCGG               CCGC^GG
MluNI        4405  TGGCCA               TGG^CCA
MscI         4405  TGGCCA               TGG^CCA
NaeI          386  GCCGGC               GCC^GGC
NdeI         6377  CATATG               CA^TATG
NgoMIV        386  GCCGGC               G^CCGGC
NheI         5670  GCTAGC               G^CTAGC
PshAI        2057  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        3641  CCGCGG               CCGC^GG
SbfI         5680  CCTGCAGG             CCTGCA^GG
ScaI          362  AGTACT               AGT^ACT
SpeI         6207  ACTAGT               A^CTAGT
SphI         5354  GCATGC               GCATG^C
Sse8387I     5680  CCTGCAGG             CCTGCA^GG
XbaI         5488  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         3990  GTCTAC               GT^[AC][GT]AC
             6151  GTCTAC              
AcyI         3354  GACGCC               G[GA]^CG[CT]C
             4360  GGCGTC              
AflII        5712  CTTAAG               C^TTAAG
             5894  CTTAAG              
AflIII        446  ACATGT               A^C[GA][CT]GT
             2602  ACATGT              
AhdI         1689  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4716  GACCAGGGGTC         
Alw44I        885  GTGCAC               G^TGCAC
             4843  GTGCAC              
AlwI         5081  GGATC                GGATC(4/5)
             5554  GGATC               
AocI         2259  CCTCAGG              CC^T[ACGT]AGG
             4917  CCTAAGG             
ApaLI         885  GTGCAC               G^TGCAC
             4843  GTGCAC              
AspEI        1689  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4716  GACCAGGGGTC         
AspI         1839  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3495  GACGGTGTC           
BfrI         5712  CTTAAG               C^TTAAG
             5894  CTTAAG              
BglI         1468  GCCACCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5027  GCCTTCAGGGC         
BsaHI        3354  GACGCC               G[GA]^CG[CT]C
             4360  GGCGTC              
BsaWI         455  ACCGGA               [AT]^CCGG[AT]
             3523  TCCGGT              
BspLU11I      446  ACATGT               A^CATGT
             2602  ACATGT              
BsrDI          17  CATTGC               GCAATG(2/0)
             5934  CATTGC              
BssSI        3430  CACGAG               CACGAG(-5/-1)
             3893  CTCGTG              
Bsu36I       2259  CCTCAGG              CC^T[ACGT]AGG
             4917  CCTAAGG             
DraI         2933  TTTAAA               TTT^AAA
             5974  TTTAAA              
Eam1105I     1689  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4716  GACCAGGGGTC         
Eco81I       2259  CCTCAGG              CC^T[ACGT]AGG
             4917  CCTAAGG             
HaeII        3567  AGCGCC               [GA]GCGC^[CT]
             5296  AGCGCT              
Hin1I        3354  GACGCC               G[GA]^CG[CT]C
             4360  GGCGTC              
HindIII      4824  AAGCTT               A^AGCTT
             5920  AAGCTT              
PflFI        1839  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3495  GACGGTGTC           
Tth111I      1839  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3495  GACGGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwNI       ApaI        ApoI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspMI       BsrBI       BsrFI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI