##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbrPI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI BstBI ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HincII HindII HpaI
MroI NotI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SfuI SnaBI
SpeI SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 1342 GTGCAC G^TGCAC
ApaLI 1342 GTGCAC G^TGCAC
Asp700I 2823 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5505 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 5324 TGATCA T^GATCA
BsaBI 2874 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5026 GCATTC GAATGC(1/-1)
Bst1107I 2193 GTATAC GTA^TAC
BstZ17I 2193 GTATAC GTA^TAC
DraI 146 TTTAAA TTT^AAA
EcoRI 1100 GAATTC G^AATTC
FbaI 5324 TGATCA T^GATCA
HgaI 5058 GCGTC GACGC(5/10)
KspI 4459 CCGCGG CCGC^GG
MamI 2874 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5384 CAATTG C^AATTG
MluI 3180 ACGCGT A^CGCGT
MunI 5384 CAATTG C^AATTG
NcoI 303 CCATGG C^CATGG
NheI 4889 GCTAGC G^CTAGC
NruI 3472 TCGCGA TCG^CGA
PflFI 5505 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 4459 CCGCGG CCGC^GG
SapI 2419 GAAGAGC GCTCTTC(1/4)
SbfI 4917 CCTGCAGG CCTGCA^GG
ScaI 2602 AGTACT AGT^ACT
SexAI 2079 ACCTGGT A^CC[AT]GGT
SgrAI 5993 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3128 GCATGC GCATG^C
Sse8387I 4917 CCTGCAGG CCTGCA^GG
Tth111I 5505 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5845 TCTAGA T^CTAGA
XmnI 2823 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 4848 GGTACC G^GTACC
6084 GGTACC
AcyI 3884 GGCGCC G[GA]^CG[CT]C
5997 GGCGCC
AflII 1798 CTTAAG C^TTAAG
4962 CTTAAG
AflIII 266 ACACGT A^C[GA][CT]GT
3180 ACGCGT
Asp718I 4848 GGTACC G^GTACC
6084 GGTACC
AvrII 251 CCTAGG C^CTAGG
1891 CCTAGG
BamHI 1567 GGATCC G^GATCC
4957 GGATCC
BbeI 3884 GGCGCC GGCGC^C
5997 GGCGCC
BcgI 3809 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5557 GCACGCTATTCG
BciVI 3352 GGATAC GTATCC(6/5)
6149 GTATCC
BfrI 1798 CTTAAG C^TTAAG
4962 CTTAAG
BlnI 251 CCTAGG C^CTAGG
1891 CCTAGG
BsaAI 267 CACGTA [CT]AC^GT[GA]
4973 TACGTG
BsaHI 3884 GGCGCC G[GA]^CG[CT]C
5997 GGCGCC
BsiEI 3475 CGACCG CG[GA][CT]^CG
5433 CGACCG
BsrBI 5496 CCGCTC CCGCTC(-3/-3)
6191 GAGCGG
BsrDI 627 CATTGC GCAATG(2/0)
4473 GCAATG
DsaI 303 CCATGG C^C[GA][CT]GG
4459 CCGCGG
EcoNI 2206 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5762 CCTTCCCCAGG
Hin1I 3884 GGCGCC G[GA]^CG[CT]C
5997 GGCGCC
KasI 3884 GGCGCC G^GCGCC
5997 GGCGCC
KpnI 4848 GGTACC GGTAC^C
6084 GGTACC
MslI 304 CATGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3262 CATCAACGTG
NaeI 500 GCCGGC GCC^GGC
5994 GCCGGC
NarI 3884 GGCGCC GG^CGCC
5997 GGCGCC
NdeI 3750 CATATG CA^TATG
4086 CATATG
NgoMIV 500 GCCGGC G^CCGGC
5994 GCCGGC
SacI 63 GAGCTC GAGCT^C
5449 GAGCTC
SfoI 3884 GGCGCC GGC^GCC
5997 GGCGCC
SmaI 432 CCCGGG CCC^GGG
4590 CCCGGG
SspI 512 AATATT AAT^ATT
714 AATATT
XmaCI 432 CCCGGG C^CCGGG
4590 CCCGGG
XmaI 432 CCCGGG C^CCGGG
4590 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII