##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbrPI
BbvCI BfrI BglI BlnI BsaHI
BsiEI BsiWI BspLU11I BssHII BstEII
BstPI Bsu36I CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HaeII Hin1I
KasI KpnI MluI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 992 TCCGGA T^CCGGA
ApaI 3523 GGGCCC GGGCC^C
BalI 4877 TGGCCA TGG^CCA
BanI 3423 GGCACC G^G[CT][GA]CC
BseAI 992 TCCGGA T^CCGGA
BsmI 2639 GAATGC GAATGC(1/-1)
BspDI 4553 ATCGAT AT^CGAT
BspEI 992 TCCGGA T^CCGGA
BspMI 3610 GCAGGT ACCTGC(4/8)
BsrBI 1844 CCGCTC CCGCTC(-3/-3)
BsrFI 911 GCCGGT [GA]^CCGG[CT]
BssSI 2006 CACGAG CACGAG(-5/-1)
Bst1107I 1740 GTATAC GTA^TAC
BstAPI 4096 GCACACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 5751 CCAGTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1740 GTATAC GTA^TAC
Cfr10I 911 GCCGGT [GA]^CCGG[CT]
ClaI 4553 ATCGAT AT^CGAT
EcoNI 3514 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 2750 AAGCTT A^AGCTT
KspI 484 CCGCGG CCGC^GG
MfeI 2147 CAATTG C^AATTG
MluNI 4877 TGGCCA TGG^CCA
MroI 992 TCCGGA T^CCGGA
MscI 4877 TGGCCA TGG^CCA
MunI 2147 CAATTG C^AATTG
NheI 1725 GCTAGC G^CTAGC
PaeR7I 3139 CTCGAG C^TCGAG
SacII 484 CCGCGG CCGC^GG
ScaI 2911 AGTACT AGT^ACT
XhoI 3139 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 2341 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3918 GAATCAATTC
AspHI 3884 GTGCTC G[AT]GC[AT]^C
4337 GAGCAC
BamHI 2994 GGATCC G^GATCC
3953 GGATCC
BcgI 2424 GCATGATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3131 GCAGAGACCTCG
BglII 3802 AGATCT A^GATCT
5899 AGATCT
BlpI 1717 GCTGAGC GC^T[ACGT]AGC
1846 GCTCAGC
Bpu1102I 1717 GCTGAGC GC^T[ACGT]AGC
1846 GCTCAGC
BsaAI 3112 TACGTA [CT]AC^GT[GA]
5679 TACGTA
BsgI 4094 CTGCAC GTGCAG(16/14)
4649 CTGCAC
BsiHKAI 3884 GTGCTC G[AT]GC[AT]^C
4337 GAGCAC
BstBI 1658 TTCGAA TT^CGAA
1820 TTCGAA
CelII 1717 GCTGAGC GC^T[ACGT]AGC
1846 GCTCAGC
DraII 2530 GGGGCCT [GA]G^G[ACGT]CC[CT]
3522 AGGGCCC
DraIII 4340 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
5126 CACACTGTG
EcoO109I 2530 GGGGCCT [GA]G^G[ACGT]CC[CT]
3522 AGGGCCC
EcoRI 4377 GAATTC G^AATTC
6493 GAATTC
HincII 211 GTTAAC GT[CT]^[GA]AC
418 GTTAAC
HindII 211 GTTAAC GT[CT]^[GA]AC
418 GTTAAC
HpaI 211 GTTAAC GTT^AAC
418 GTTAAC
NspV 1658 TTCGAA TT^CGAA
1820 TTCGAA
SapI 3376 GAAGAGC GCTCTTC(1/4)
3451 GAAGAGC
SfuI 1658 TTCGAA TT^CGAA
1820 TTCGAA
SnaBI 3112 TACGTA TAC^GTA
5679 TACGTA
SphI 1050 GCATGC GCATG^C
5434 GCATGC
StuI 895 AGGCCT AGG^CCT
1912 AGGCCT
XmnI 2341 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3918 GAATCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AhdI AluI AlwI AlwNI ApoI
AspEI AvaI AvaII BanII BbsI
BbvI BciVI BclI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI Eam1105I EarI
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII