##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
AocI ApaI ApaLI AscI Asp718I
AviII AvrII BalI BbrPI BcgI
BclI BglI BglII BlnI BlpI
Bpu1102I BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspMI BsrGI BssHII
BssSI BstEII BstPI BstXI Bsu36I
CelII ClaI CpoI DrdI DsaI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MluNI MroI
MscI NaeI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacI SacII
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AclI 2278 AACGTT AA^CGTT
AcsI 2268 AAATTT [GA]^AATT[CT]
AflII 1350 CTTAAG C^TTAAG
AhdI 1822 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2340 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 532 AGCGCT AGC^GCT
ApoI 2268 AAATTT [GA]^AATT[CT]
Asp700I 1884 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1822 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 385 CCCGAG C^[CT]CG[GA]G
BanII 488 GGGCTC G[GA]GC[CT]^C
BbeI 396 GGCGCC GGCGC^C
BbsI 1592 GTCTTC GAAGAC(2/6)
BbvCI 414 GCTGAGG CCTCAGC(-5/-2)
BciVI 2261 GTATCC GTATCC(6/5)
BcnI 184 CCGGG CC^[GC]GG
BfrI 1350 CTTAAG C^TTAAG
BpuAI 1592 GTCTTC GAAGAC(2/6)
BsaAI 928 TACGTG [CT]AC^GT[GA]
BsaI 432 GGTCTC GGTCTC(1/5)
BsaWI 341 ACCGGA [AT]^CCGG[AT]
BsgI 1659 GTGCAG GTGCAG(16/14)
BsiEI 100 CGGCCG CG[GA][CT]^CG
BsoBI 385 CCCGAG C^[CT]CG[GA]G
BspHI 2060 TCATGA T^CATGA
BspLU11I 1145 ACATGT A^CATGT
BsrFI 429 GCCGGT [GA]^CCGG[CT]
Bst1107I 1743 GTATAC GTA^TAC
BstAPI 1206 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1993 TTCGAA TT^CGAA
BstZ17I 1743 GTATAC GTA^TAC
Cfr10I 429 GCCGGT [GA]^CCGG[CT]
DraIII 1477 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 100 CGGCCG [CT]^GGCC[GA]
EagI 100 CGGCCG C^GGCCG
Eam1105I 1822 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 100 CGGCCG C^GGCCG
Eco47III 532 AGCGCT AGC^GCT
Eco52I 100 CGGCCG C^GGCCG
EcoT14I 2128 CCTTGG C^C[AT][AT]GG
KasI 396 GGCGCC G^GCGCC
MfeI 1706 CAATTG C^AATTG
MluI 1086 ACGCGT A^CGCGT
MslI 2061 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1706 CAATTG C^AATTG
NarI 396 GGCGCC GG^CGCC
NciI 184 CCGGG CC^[GC]GG
NdeI 2191 CATATG CA^TATG
NspI 1145 ACATGT [GA]CATG^[CT]
NspV 1993 TTCGAA TT^CGAA
PpuMI 541 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2278 AACGTT AA^CGTT
RcaI 2060 TCATGA T^CATGA
SalI 841 GTCGAC G^TCGAC
SapI 1579 GCTCTTC GCTCTTC(1/4)
ScaI 997 AGTACT AGT^ACT
SfoI 396 GGCGCC GGC^GCC
SfuI 1993 TTCGAA TT^CGAA
SmlI 1350 CTTAAG C^T[CT][GA]AG
StyI 2128 CCTTGG C^C[AT][AT]GG
XcmI 1705 CCAATTGGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1884 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 396 GGCGCC G[GA]^CG[CT]C
1812 GGCGTC
AfaI 998 GTAC GT^AC
1114 GTAC
AflIII 1086 ACGCGT A^C[GA][CT]GT
1145 ACATGT
AseI 1164 ATTAAT AT^TAAT
1407 ATTAAT
AsnI 1164 ATTAAT AT^TAAT
1407 ATTAAT
AspHI 751 GTGCTC G[AT]GC[AT]^C
2068 GTGCTC
AspI 311 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
509 GACGGAGTC
AvaII 542 GGTCC G^G[AT]CC
1374 GGACC
BanI 396 GGCGCC G^G[CT][GA]CC
2222 GGCACC
BfaI 1605 CTAG C^TAG
2330 CTAG
BsaHI 396 GGCGCC G[GA]^CG[CT]C
1812 GGCGTC
BsiHKAI 751 GTGCTC G[AT]GC[AT]^C
2068 GTGCTC
BsmBI 507 GAGACG CGTCTC(1/5)
1969 GAGACG
DraII 541 GGGTCCT [GA]G^G[ACGT]CC[CT]
1391 AGGCCCT
EcoO109I 541 GGGTCCT [GA]G^G[ACGT]CC[CT]
1391 AGGCCCT
Hin1I 396 GGCGCC G[GA]^CG[CT]C
1812 GGCGTC
MaeI 1605 CTAG C^TAG
2330 CTAG
PflFI 311 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
509 GACGGAGTC
PleI 93 GACTC GAGTC(4/5)
513 GAGTC
PshBI 1164 ATTAAT AT^TAAT
1407 ATTAAT
PstI 650 CTGCAG CTGCA^G
2308 CTGCAG
PvuII 412 CAGCTG CAG^CTG
1271 CAGCTG
RsaI 998 GTAC GT^AC
1114 GTAC
Tth111I 311 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
509 GACGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AccII AciI AluI AlwI
BamHI BbvI BmyI BpmI BsaBI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII