##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbeI BlnI BsaHI
BsiWI BsmBI BspDI BsrDI BssHII
Bst1107I BstBI BstZ17I ClaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI Hin1I KasI KpnI
MluI NarI NdeI NotI NruI
NspV PacI PinAI PmeI Psp1406I
PvuI SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 577 TCCGGA T^CCGGA
Alw44I 2702 GTGCAC G^TGCAC
ApaI 609 GGGCCC GGGCC^C
ApaLI 2702 GTGCAC G^TGCAC
AseI 1120 ATTAAT AT^TAAT
AsnI 1120 ATTAAT AT^TAAT
AvaI 3930 CTCGAG C^[CT]CG[GA]G
BamHI 512 GGATCC G^GATCC
BbrPI 1550 CACGTG CAC^GTG
BbvCI 1276 GCTGAGG CCTCAGC(-5/-2)
BcgI 1479 CGAGAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2562 GGATAC GTATCC(6/5)
BsaAI 1550 CACGTG [CT]AC^GT[GA]
BsaBI 2936 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 577 TCCGGA [AT]^CCGG[AT]
BseAI 577 TCCGGA T^CCGGA
BsiEI 44 CGGTCG CG[GA][CT]^CG
BsoBI 3930 CTCGAG C^[CT]CG[GA]G
BspEI 577 TCCGGA T^CCGGA
BsrBI 72 GAGCGG CCGCTC(-3/-3)
BssSI 3833 CACGAG CACGAG(-5/-1)
BstEII 4886 GGTGACC G^GT[ACGT]ACC
BstPI 4886 GGTGACC G^GT[ACGT]ACC
EcoNI 3196 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4886 GGTGACC G^GT[ACGT]ACC
EcoRI 5807 GAATTC G^AATTC
HgaI 184 GACGC GACGC(5/10)
KspI 86 CCGCGG CCGC^GG
MamI 2936 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 929 CAATTG C^AATTG
MroI 577 TCCGGA T^CCGGA
MunI 929 CAATTG C^AATTG
NaeI 4 GCCGGC GCC^GGC
NcoI 1812 CCATGG C^CATGG
NgoMIV 4 GCCGGC G^CCGGC
NheI 1698 GCTAGC G^CTAGC
PaeR7I 3930 CTCGAG C^TCGAG
PmaCI 1550 CACGTG CAC^GTG
PmlI 1550 CACGTG CAC^GTG
PshAI 1965 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1120 ATTAAT AT^TAAT
PstI 1502 CTGCAG CTGCA^G
SacII 86 CCGCGG CCGC^GG
SapI 2915 GCTCTTC GCTCTTC(1/4)
ScaI 2163 AGTACT AGT^ACT
SpeI 3376 ACTAGT A^CTAGT
SspI 1171 AATATT AAT^ATT
StuI 1316 AGGCCT AGG^CCT
XbaI 3509 TCTAGA T^CTAGA
XhoI 3930 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 2524 CTTAAG C^TTAAG
5503 CTTAAG
Asp700I 1609 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1867 GAATGCCTTC
BcnI 831 CCGGG CC^[GC]GG
870 CCCGG
BfrI 2524 CTTAAG C^TTAAG
5503 CTTAAG
BglII 437 AGATCT A^GATCT
2941 AGATCT
BlpI 57 GCTCAGC GC^T[ACGT]AGC
2539 GCTCAGC
Bpu1102I 57 GCTCAGC GC^T[ACGT]AGC
2539 GCTCAGC
BspLU11I 266 ACATGT A^CATGT
2574 ACATGT
BspMI 848 ACCTGC ACCTGC(4/8)
3070 GCAGGT
BsrFI 4 GCCGGC [GA]^CCGG[CT]
3099 ACCGGC
CelII 57 GCTCAGC GC^T[ACGT]AGC
2539 GCTCAGC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
3099 ACCGGC
CpoI 89 CGGTCCG CG^G[AT]CCG
148 CGGACCG
DraIII 3796 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5001 CACTGGGTG
EcoT22I 1163 ATGCAT ATGCA^T
4067 ATGCAT
HaeII 143 AGCGCC [GA]GCGC^[CT]
1417 GGCGCT
HpaI 121 GTTAAC GTT^AAC
4310 GTTAAC
MaeII 658 ACGT A^CGT
1551 ACGT
NciI 831 CCGGG CC^[GC]GG
870 CCCGG
NsiI 1163 ATGCAT ATGCA^T
4067 ATGCAT
PpuMI 866 AGGACCC [GA]G^G[AT]CC[CT]
4097 AGGACCC
RsrII 89 CGGTCCG CG^G[AT]CCG
148 CGGACCG
XmnI 1609 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1867 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AspI AvaII BalI
BanI BanII BbsI BbvI BclI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII