################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BbeI BlnI BsaHI BsiWI BsmBI BspDI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI Hin1I KasI KpnI MluI NarI NdeI NotI NruI NspV PacI PinAI PmeI Psp1406I PvuI SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 577 TCCGGA T^CCGGA Alw44I 2702 GTGCAC G^TGCAC ApaI 609 GGGCCC GGGCC^C ApaLI 2702 GTGCAC G^TGCAC AseI 1120 ATTAAT AT^TAAT AsnI 1120 ATTAAT AT^TAAT AvaI 3930 CTCGAG C^[CT]CG[GA]G BamHI 512 GGATCC G^GATCC BbrPI 1550 CACGTG CAC^GTG BbvCI 1276 GCTGAGG CCTCAGC(-5/-2) BcgI 1479 CGAGAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2562 GGATAC GTATCC(6/5) BsaAI 1550 CACGTG [CT]AC^GT[GA] BsaBI 2936 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 577 TCCGGA [AT]^CCGG[AT] BseAI 577 TCCGGA T^CCGGA BsiEI 44 CGGTCG CG[GA][CT]^CG BsoBI 3930 CTCGAG C^[CT]CG[GA]G BspEI 577 TCCGGA T^CCGGA BsrBI 72 GAGCGG CCGCTC(-3/-3) BssSI 3833 CACGAG CACGAG(-5/-1) BstEII 4886 GGTGACC G^GT[ACGT]ACC BstPI 4886 GGTGACC G^GT[ACGT]ACC EcoNI 3196 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4886 GGTGACC G^GT[ACGT]ACC EcoRI 5807 GAATTC G^AATTC HgaI 184 GACGC GACGC(5/10) KspI 86 CCGCGG CCGC^GG MamI 2936 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 929 CAATTG C^AATTG MroI 577 TCCGGA T^CCGGA MunI 929 CAATTG C^AATTG NaeI 4 GCCGGC GCC^GGC NcoI 1812 CCATGG C^CATGG NgoMIV 4 GCCGGC G^CCGGC NheI 1698 GCTAGC G^CTAGC PaeR7I 3930 CTCGAG C^TCGAG PmaCI 1550 CACGTG CAC^GTG PmlI 1550 CACGTG CAC^GTG PshAI 1965 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1120 ATTAAT AT^TAAT PstI 1502 CTGCAG CTGCA^G SacII 86 CCGCGG CCGC^GG SapI 2915 GCTCTTC GCTCTTC(1/4) ScaI 2163 AGTACT AGT^ACT SpeI 3376 ACTAGT A^CTAGT SspI 1171 AATATT AAT^ATT StuI 1316 AGGCCT AGG^CCT XbaI 3509 TCTAGA T^CTAGA XhoI 3930 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflII 2524 CTTAAG C^TTAAG 5503 CTTAAG Asp700I 1609 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1867 GAATGCCTTC BcnI 831 CCGGG CC^[GC]GG 870 CCCGG BfrI 2524 CTTAAG C^TTAAG 5503 CTTAAG BglII 437 AGATCT A^GATCT 2941 AGATCT BlpI 57 GCTCAGC GC^T[ACGT]AGC 2539 GCTCAGC Bpu1102I 57 GCTCAGC GC^T[ACGT]AGC 2539 GCTCAGC BspLU11I 266 ACATGT A^CATGT 2574 ACATGT BspMI 848 ACCTGC ACCTGC(4/8) 3070 GCAGGT BsrFI 4 GCCGGC [GA]^CCGG[CT] 3099 ACCGGC CelII 57 GCTCAGC GC^T[ACGT]AGC 2539 GCTCAGC Cfr10I 4 GCCGGC [GA]^CCGG[CT] 3099 ACCGGC CpoI 89 CGGTCCG CG^G[AT]CCG 148 CGGACCG DraIII 3796 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 5001 CACTGGGTG EcoT22I 1163 ATGCAT ATGCA^T 4067 ATGCAT HaeII 143 AGCGCC [GA]GCGC^[CT] 1417 GGCGCT HpaI 121 GTTAAC GTT^AAC 4310 GTTAAC MaeII 658 ACGT A^CGT 1551 ACGT NciI 831 CCGGG CC^[GC]GG 870 CCCGG NsiI 1163 ATGCAT ATGCA^T 4067 ATGCAT PpuMI 866 AGGACCC [GA]G^G[AT]CC[CT] 4097 AGGACCC RsrII 89 CGGTCCG CG^G[AT]CCG 148 CGGACCG XmnI 1609 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1867 GAATGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaII BalI BanI BanII BbsI BbvI BclI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII