##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BbeI BbsI BfrI BpuAI BsaBI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraIII Eam1105I Eco47III EcoO65I FseI
FspI HpaI KasI MamI MluI
NarI NheI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4153 GGTACC G^GTACC
AgeI 4594 ACCGGT A^CCGGT
Asp718I 4153 GGTACC G^GTACC
AspI 1709 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2463 GGATCC G^GATCC
BbvCI 4998 GCTGAGG CCTCAGC(-5/-2)
BciVI 4520 GGATAC GTATCC(6/5)
BclI 1968 TGATCA T^GATCA
BglII 4413 AGATCT A^GATCT
BlpI 860 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 860 GCTGAGC GC^T[ACGT]AGC
BsiEI 1918 CGGCCG CG[GA][CT]^CG
BsrDI 4004 GCAATG GCAATG(2/0)
CelII 860 GCTGAGC GC^T[ACGT]AGC
CpoI 1055 CGGACCG CG^G[AT]CCG
DraI 4748 TTTAAA TTT^AAA
EagI 1918 CGGCCG C^GGCCG
EclXI 1918 CGGCCG C^GGCCG
Eco52I 1918 CGGCCG C^GGCCG
EcoNI 1120 CCTGATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3551 GATATC GAT^ATC
FbaI 1968 TGATCA T^GATCA
HincII 1715 GTCAAC GT[CT]^[GA]AC
HindII 1715 GTCAAC GT[CT]^[GA]AC
KpnI 4153 GGTACC GGTAC^C
KspI 1069 CCGCGG CCGC^GG
MfeI 3915 CAATTG C^AATTG
MunI 3915 CAATTG C^AATTG
NdeI 5405 CATATG CA^TATG
PaeR7I 549 CTCGAG C^TCGAG
PflFI 1709 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4594 ACCGGT A^CCGGT
RsrII 1055 CGGACCG CG^G[AT]CCG
SacII 1069 CCGCGG CCGC^GG
SphI 1441 GCATGC GCATG^C
SspI 5162 AATATT AAT^ATT
Tth111I 1709 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 549 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 91 GACGCC G[GA]^CG[CT]C
239 GACGCC
Alw44I 1787 GTGCAC G^TGCAC
2813 GTGCAC
ApaLI 1787 GTGCAC G^TGCAC
2813 GTGCAC
AvrII 3169 CCTAGG C^CTAGG
3420 CCTAGG
BglI 497 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4030 GCCATGGAGGC
BlnI 3169 CCTAGG C^CTAGG
3420 CCTAGG
BsaHI 91 GACGCC G[GA]^CG[CT]C
239 GACGCC
Bsp1407I 1343 TGTACA T^GTACA
3012 TGTACA
BspLU11I 4683 ACATGT A^CATGT
5440 ACATGT
BsrBI 863 GAGCGG CCGCTC(-3/-3)
1868 GAGCGG
BsrGI 1343 TGTACA T^GTACA
3012 TGTACA
BstAPI 100 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
481 GCAGACCTTGC
EcoT22I 4583 ATGCAT ATGCA^T
4929 ATGCAT
Hin1I 91 GACGCC G[GA]^CG[CT]C
239 GACGCC
NaeI 881 GCCGGC GCC^GGC
1920 GCCGGC
NgoMIV 881 GCCGGC G^CCGGC
1920 GCCGGC
NsiI 4583 ATGCAT ATGCA^T
4929 ATGCAT
SapI 1690 GAAGAGC GCTCTTC(1/4)
4346 GAAGAGC
SmaI 289 CCCGGG CCC^GGG
462 CCCGGG
SspBI 1343 TGTACA T^GTACA
3012 TGTACA
StuI 2055 AGGCCT AGG^CCT
3417 AGGCCT
XmaCI 289 CCCGGG C^CCGGG
462 CCCGGG
XmaI 289 CCCGGG C^CCGGG
462 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcgI BcnI BfaI
BmyI BpmI BsaAI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspEI BspHI BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI