##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AcyI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI BalI BamHI BbeI BbrPI
BclI BglI BglII BlpI Bpu1102I
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BsrFI BssHII BssSI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV FauI
FbaI FseI HaeII Hin1I KasI
KspI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmlI PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 451 AACGTT AA^CGTT
AflII 1406 CTTAAG C^TTAAG
AlwNI 866 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 5381 CCTAGG C^CTAGG
BanII 3129 GAGCTC G[GA]GC[CT]^C
BbvCI 4037 GCTGAGG CCTCAGC(-5/-2)
BcgI 4256 CGAATGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 354 CCCGG CC^[GC]GG
BfrI 1406 CTTAAG C^TTAAG
BlnI 5381 CCTAGG C^CTAGG
BsaAI 1760 CACGTA [CT]AC^GT[GA]
BsaBI 3282 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 1833 GAGCGG CCGCTC(-3/-3)
BstEII 5004 GGTGACC G^GT[ACGT]ACC
BstPI 5004 GGTGACC G^GT[ACGT]ACC
DraIII 5743 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 744 GACTAGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1210 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5004 GGTGACC G^GT[ACGT]ACC
HapII 355 CCGG C^CGG
HgaI 2873 GCGTC GACGC(5/10)
HpaI 96 GTTAAC GTT^AAC
HpaII 355 CCGG C^CGG
MamI 3282 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 355 CCGG C^CGG
NciI 354 CCCGG CC^[GC]GG
NcoI 4027 CCATGG C^CATGG
PmeI 5734 GTTTAAAC GTTT^AAAC
PshAI 3015 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 451 AACGTT AA^CGTT
SacI 3129 GAGCTC GAGCT^C
SexAI 5744 ACCAGGT A^CC[AT]GGT
SpeI 5358 ACTAGT A^CTAGT
SphI 5663 GCATGC GCATG^C
StuI 4967 AGGCCT AGG^CCT
SwaI 4367 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1525 GGTACC G^GTACC
5328 GGTACC
Asp718I 1525 GGTACC G^GTACC
5328 GGTACC
AviII 2090 TGCGCA TGC^GCA
3114 TGCGCA
BciVI 1173 GGATAC GTATCC(6/5)
1592 GGATAC
Bsp1407I 4718 TGTACA T^GTACA
5055 TGTACA
BspMI 2941 ACCTGC ACCTGC(4/8)
5331 ACCTGC
BsrGI 4718 TGTACA T^GTACA
5055 TGTACA
Bst1107I 1810 GTATAC GTA^TAC
5973 GTATAC
BstBI 1449 TTCGAA TT^CGAA
5709 TTCGAA
BstXI 1468 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2841 CCAGCACTGTGG
BstZ17I 1810 GTATAC GTA^TAC
5973 GTATAC
CfoI 2091 GCGC GCG^C
3115 GCGC
DsaI 212 CCACGG C^C[GA][CT]GG
4027 CCATGG
EcoRI 1928 GAATTC G^AATTC
5712 GAATTC
EcoT22I 1199 ATGCAT ATGCA^T
2294 ATGCAT
FspI 2090 TGCGCA TGC^GCA
3114 TGCGCA
HhaI 2091 GCGC GCG^C
3115 GCGC
HinP1I 2091 GCGC G^CGC
3115 GCGC
HincII 96 GTTAAC GT[CT]^[GA]AC
296 GTTGAC
HindII 96 GTTAAC GT[CT]^[GA]AC
296 GTTGAC
KpnI 1525 GGTACC GGTAC^C
5328 GGTACC
MfeI 301 CAATTG C^AATTG
2868 CAATTG
MunI 301 CAATTG C^AATTG
2868 CAATTG
NsiI 1199 ATGCAT ATGCA^T
2294 ATGCAT
NspV 1449 TTCGAA TT^CGAA
5709 TTCGAA
PflMI 1516 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5717 CCAGGTAATGG
PpuMI 1187 AGGACCT [GA]G^G[AT]CC[CT]
1990 GGGACCT
ScaI 2813 AGTACT AGT^ACT
4514 AGTACT
SfuI 1449 TTCGAA TT^CGAA
5709 TTCGAA
SspBI 4718 TGTACA T^GTACA
5055 TGTACA
Van91I 1516 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5717 CCAGGTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BanI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI