##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AspEI AviII BbrPI BfrI
BsaAI BsaBI BsiEI BsiWI BsmI
BspDI BssHII BstBI ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoT22I
FseI FspI HpaI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI ScaI SfuI SgrAI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1389 TCCGGA T^CCGGA
AseI 5404 ATTAAT AT^TAAT
AsnI 5404 ATTAAT AT^TAAT
BciVI 1274 GGATAC GTATCC(6/5)
BseAI 1389 TCCGGA T^CCGGA
BspEI 1389 TCCGGA T^CCGGA
Bst1107I 1267 GTATAC GTA^TAC
BstEII 3271 GGTGACC G^GT[ACGT]ACC
BstPI 3271 GGTGACC G^GT[ACGT]ACC
BstZ17I 1267 GTATAC GTA^TAC
EcoO65I 3271 GGTGACC G^GT[ACGT]ACC
EcoRI 4366 GAATTC G^AATTC
EcoRV 5109 GATATC GAT^ATC
HindIII 2596 AAGCTT A^AGCTT
KspI 658 CCGCGG CCGC^GG
MroI 1389 TCCGGA T^CCGGA
NdeI 2632 CATATG CA^TATG
PaeR7I 4988 CTCGAG C^TCGAG
PshBI 5404 ATTAAT AT^TAAT
SacII 658 CCGCGG CCGC^GG
SapI 83 GCTCTTC GCTCTTC(1/4)
SphI 42 GCATGC GCATG^C
SspI 2661 AATATT AAT^ATT
XhoI 4988 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 3425 GTGCAC G^TGCAC
3476 GTGCAC
Aor51HI 150 AGCGCT AGC^GCT
5710 AGCGCT
ApaLI 3425 GTGCAC G^TGCAC
3476 GTGCAC
Asp700I 2757 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4344 GAATGGCTTC
AspI 528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6631 GACTCCGTC
AvrII 1906 CCTAGG C^CTAGG
2917 CCTAGG
BamHI 95 GGATCC G^GATCC
1878 GGATCC
BbvCI 3041 GCTGAGG CCTCAGC(-5/-2)
6540 GCTGAGG
BcgI 187 CGAGCGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5751 GCAATCTTCTCG
BclI 2218 TGATCA T^GATCA
5245 TGATCA
BlnI 1906 CCTAGG C^CTAGG
2917 CCTAGG
BsgI 146 GTGCAG GTGCAG(16/14)
3919 GTGCAG
BspHI 4283 TCATGA T^CATGA
5392 TCATGA
BsrBI 1008 CCGCTC CCGCTC(-3/-3)
5166 GAGCGG
BstAPI 3792 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5784 GCAGATCGTGC
DraI 2029 TTTAAA TTT^AAA
5115 TTTAAA
DraIII 5736 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
6069 CACCATGTG
DrdI 2555 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3355 GACGTGCTGGTC
Eco47III 150 AGCGCT AGC^GCT
5710 AGCGCT
FbaI 2218 TGATCA T^GATCA
5245 TGATCA
HincII 2637 GTTGAC GT[CT]^[GA]AC
3808 GTCAAC
HindII 2637 GTTGAC GT[CT]^[GA]AC
3808 GTCAAC
PflFI 528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6631 GACTCCGTC
RcaI 4283 TCATGA T^CATGA
5392 TCATGA
SfiI 2494 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4422 GGCCCAGCTGGCC
StuI 1694 AGGCCT AGG^CCT
3016 AGGCCT
Tth111I 528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6631 GACTCCGTC
XmnI 2757 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4344 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI Sse8387I SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI