################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AclI AflII AgeI AhdI AscI AspEI AviII BbrPI BfrI BsaAI BsaBI BsiEI BsiWI BsmI BspDI BssHII BstBI ClaI CpoI EagI Eam1105I EclXI Eco52I EcoT22I FseI FspI HpaI MamI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AccIII 1389 TCCGGA T^CCGGA AseI 5404 ATTAAT AT^TAAT AsnI 5404 ATTAAT AT^TAAT BciVI 1274 GGATAC GTATCC(6/5) BseAI 1389 TCCGGA T^CCGGA BspEI 1389 TCCGGA T^CCGGA Bst1107I 1267 GTATAC GTA^TAC BstEII 3271 GGTGACC G^GT[ACGT]ACC BstPI 3271 GGTGACC G^GT[ACGT]ACC BstZ17I 1267 GTATAC GTA^TAC EcoO65I 3271 GGTGACC G^GT[ACGT]ACC EcoRI 4366 GAATTC G^AATTC EcoRV 5109 GATATC GAT^ATC HindIII 2596 AAGCTT A^AGCTT KspI 658 CCGCGG CCGC^GG MroI 1389 TCCGGA T^CCGGA NdeI 2632 CATATG CA^TATG PaeR7I 4988 CTCGAG C^TCGAG PshBI 5404 ATTAAT AT^TAAT SacII 658 CCGCGG CCGC^GG SapI 83 GCTCTTC GCTCTTC(1/4) SphI 42 GCATGC GCATG^C SspI 2661 AATATT AAT^ATT XhoI 4988 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 3425 GTGCAC G^TGCAC 3476 GTGCAC Aor51HI 150 AGCGCT AGC^GCT 5710 AGCGCT ApaLI 3425 GTGCAC G^TGCAC 3476 GTGCAC Asp700I 2757 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4344 GAATGGCTTC AspI 528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6631 GACTCCGTC AvrII 1906 CCTAGG C^CTAGG 2917 CCTAGG BamHI 95 GGATCC G^GATCC 1878 GGATCC BbvCI 3041 GCTGAGG CCTCAGC(-5/-2) 6540 GCTGAGG BcgI 187 CGAGCGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5751 GCAATCTTCTCG BclI 2218 TGATCA T^GATCA 5245 TGATCA BlnI 1906 CCTAGG C^CTAGG 2917 CCTAGG BsgI 146 GTGCAG GTGCAG(16/14) 3919 GTGCAG BspHI 4283 TCATGA T^CATGA 5392 TCATGA BsrBI 1008 CCGCTC CCGCTC(-3/-3) 5166 GAGCGG BstAPI 3792 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5784 GCAGATCGTGC DraI 2029 TTTAAA TTT^AAA 5115 TTTAAA DraIII 5736 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG 6069 CACCATGTG DrdI 2555 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3355 GACGTGCTGGTC Eco47III 150 AGCGCT AGC^GCT 5710 AGCGCT FbaI 2218 TGATCA T^GATCA 5245 TGATCA HincII 2637 GTTGAC GT[CT]^[GA]AC 3808 GTCAAC HindII 2637 GTTGAC GT[CT]^[GA]AC 3808 GTCAAC PflFI 528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6631 GACTCCGTC RcaI 4283 TCATGA T^CATGA 5392 TCATGA SfiI 2494 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4422 GGCCCAGCTGGCC StuI 1694 AGGCCT AGG^CCT 3016 AGGCCT Tth111I 528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6631 GACTCCGTC XmnI 2757 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4344 GAATGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 153 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI Sse8387I SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI