##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
Acc65I AclI AscI AseI AsnI
Asp718I AviII BciVI BsaBI BsiWI
BsrBI BssHII BstBI CpoI EcoNI
EcoRI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NspV
PacI PmeI PshAI PshBI Psp1406I
RsrII SacII SapI SexAI SfuI
SgrAI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 6635 GACGTC GACGT^C
AccIII 1516 TCCGGA T^CCGGA
AgeI 3799 ACCGGT A^CCGGT
AocI 2379 CCTAAGG CC^T[ACGT]AGG
ApaI 3239 GGGCCC GGGCC^C
BamHI 2552 GGATCC G^GATCC
BclI 5385 TGATCA T^GATCA
BseAI 1516 TCCGGA T^CCGGA
BspEI 1516 TCCGGA T^CCGGA
Bst1107I 4282 GTATAC GTA^TAC
BstEII 1139 GGTGACC G^GT[ACGT]ACC
BstPI 1139 GGTGACC G^GT[ACGT]ACC
BstZ17I 4282 GTATAC GTA^TAC
Bsu36I 2379 CCTAAGG CC^T[ACGT]AGG
DraIII 3893 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1857 GACTTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2379 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1139 GGTGACC G^GT[ACGT]ACC
FbaI 5385 TGATCA T^GATCA
MroI 1516 TCCGGA T^CCGGA
NaeI 2953 GCCGGC GCC^GGC
NcoI 4076 CCATGG C^CATGG
NgoMIV 2953 GCCGGC G^CCGGC
NruI 795 TCGCGA TCG^CGA
PflMI 156 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3799 ACCGGT A^CCGGT
PvuI 1890 CGATCG CGAT^CG
SalI 3416 GTCGAC G^TCGAC
SbfI 3903 CCTGCAGG CCTGCA^GG
SfiI 3775 GGCCCGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3903 CCTGCAGG CCTGCA^GG
SspI 2743 AATATT AAT^ATT
Van91I 156 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3416 GTCGAC GT^[AC][GT]AC
4282 GTATAC
AflII 4 CTTAAG C^TTAAG
6173 CTTAAG
Alw44I 265 GTGCAC G^TGCAC
2439 GTGCAC
ApaLI 265 GTGCAC G^TGCAC
2439 GTGCAC
AspI 1895 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5181 GACACTGTC
AvrII 420 CCTAGG C^CTAGG
3446 CCTAGG
BbrPI 3973 CACGTG CAC^GTG
5300 CACGTG
BbvCI 1265 CCTCAGC CCTCAGC(-5/-2)
4040 GCTGAGG
BcgI 2843 CGAGGCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3659 CGACCCCGATGC
BfrI 4 CTTAAG C^TTAAG
6173 CTTAAG
BglII 4351 AGATCT A^GATCT
6660 AGATCT
BlnI 420 CCTAGG C^CTAGG
3446 CCTAGG
BsaWI 1516 TCCGGA [AT]^CCGG[AT]
3799 ACCGGT
BsmBI 6637 CGTCTC CGTCTC(1/5)
4808 GAGACG
BstAPI 3518 GCACGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6234 GCAGAGGCTGC
EagI 3783 CGGCCG C^GGCCG
3838 CGGCCG
EclXI 3783 CGGCCG C^GGCCG
3838 CGGCCG
Eco52I 3783 CGGCCG C^GGCCG
3838 CGGCCG
EcoRV 2316 GATATC GAT^ATC
2402 GATATC
PflFI 1895 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5181 GACACTGTC
PmaCI 3973 CACGTG CAC^GTG
5300 CACGTG
PmlI 3973 CACGTG CAC^GTG
5300 CACGTG
ScaI 859 AGTACT AGT^ACT
1357 AGTACT
SnaBI 4239 TACGTA TAC^GTA
4285 TACGTA
SphI 308 GCATGC GCATG^C
6270 GCATGC
Tth111I 1895 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5181 GACACTGTC
XcmI 3951 CCAGGGTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4116 CCAGAGGAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
Aor51HI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I ClaI DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI Eco47III EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII XmaCI XmaI XmnI