##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AgeI AhdI AlwNI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII BanI BbeI
BcgI BclI BcnI BglI BsaBI
BsaHI BseRI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CfoI Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI Ksp632I
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 780 TCCGGA T^CCGGA
AflII 1571 CTTAAG C^TTAAG
Alw44I 1085 GTGCAC G^TGCAC
AocI 151 CCTCAGG CC^T[ACGT]AGG
ApaLI 1085 GTGCAC G^TGCAC
AspHI 1085 GTGCAC G[AT]GC[AT]^C
BalI 1503 TGGCCA TGG^CCA
BamHI 1035 GGATCC G^GATCC
BanII 1628 GGGCTC G[GA]GC[CT]^C
BbrPI 1199 CACGTG CAC^GTG
BbsI 8 GTCTTC GAAGAC(2/6)
BbvCI 214 CCTCAGC CCTCAGC(-5/-2)
BciVI 685 GGATAC GTATCC(6/5)
BfrI 1571 CTTAAG C^TTAAG
BglII 899 AGATCT A^GATCT
BlpI 1630 GCTCAGC GC^T[ACGT]AGC
BpmI 799 CTGGAG CTGGAG(16/14)
Bpu1102I 1630 GCTCAGC GC^T[ACGT]AGC
BpuAI 8 GTCTTC GAAGAC(2/6)
BsaI 435 GAGACC GGTCTC(1/5)
BsaWI 780 TCCGGA [AT]^CCGG[AT]
BseAI 780 TCCGGA T^CCGGA
BsgI 171 CTGCAC GTGCAG(16/14)
BsiHKAI 1085 GTGCAC G[AT]GC[AT]^C
BsmI 1327 GCATTC GAATGC(1/-1)
BspEI 780 TCCGGA T^CCGGA
BspMI 518 ACCTGC ACCTGC(4/8)
BstAPI 1217 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 151 CCTCAGG CC^T[ACGT]AGG
CelII 1630 GCTCAGC GC^T[ACGT]AGC
DraIII 1519 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1245 CCATGG C^C[GA][CT]GG
EaeI 1503 TGGCCA [CT]^GGCC[GA]
Eco81I 151 CCTCAGG CC^T[ACGT]AGG
EcoT22I 954 ATGCAT ATGCA^T
HapII 781 CCGG C^CGG
HincII 1229 GTTAAC GT[CT]^[GA]AC
HindII 1229 GTTAAC GT[CT]^[GA]AC
HpaI 1229 GTTAAC GTT^AAC
HpaII 781 CCGG C^CGG
MluNI 1503 TGGCCA TGG^CCA
MroI 780 TCCGGA T^CCGGA
MscI 1503 TGGCCA TGG^CCA
MspI 781 CCGG C^CGG
NcoI 1245 CCATGG C^CATGG
NdeI 1723 CATATG CA^TATG
NsiI 954 ATGCAT ATGCA^T
PmaCI 1199 CACGTG CAC^GTG
PmlI 1199 CACGTG CAC^GTG
ScaI 471 AGTACT AGT^ACT
TaqI 449 TCGA T^CGA
TthHB8I 449 TCGA T^CGA
XcmI 1702 CCAATTTGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1095 AAATTT [GA]^AATT[CT]
1435 AAATTC
ApoI 1095 AAATTT [GA]^AATT[CT]
1435 AAATTC
AseI 713 ATTAAT AT^TAAT
1367 ATTAAT
AsnI 713 ATTAAT AT^TAAT
1367 ATTAAT
Asp700I 345 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAAGCATTC
AvaII 103 GGACC G^G[AT]CC
854 GGACC
AvrII 869 CCTAGG C^CTAGG
1040 CCTAGG
BlnI 869 CCTAGG C^CTAGG
1040 CCTAGG
Bsp1407I 198 TGTACA T^GTACA
768 TGTACA
BsrGI 198 TGTACA T^GTACA
768 TGTACA
Cac8I 1150 GCTTGC GC[ACGT]^[ACGT]GC
1635 GCCAGC
DraI 254 TTTAAA TTT^AAA
644 TTTAAA
EcoNI 151 CCTCAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
642 CCTTTAAAAGG
HindIII 293 AAGCTT A^AGCTT
1668 AAGCTT
MspA1I 168 CAGCTG C[AC]G^C[GT]G
858 CAGCTG
PshBI 713 ATTAAT AT^TAAT
1367 ATTAAT
PstI 1145 CTGCAG CTGCA^G
1224 CTGCAG
PvuII 168 CAGCTG CAG^CTG
858 CAGCTG
SfaNI 415 GCATC GCATC(5/9)
956 GCATC
SpeI 14 ACTAGT A^CTAGT
1786 ACTAGT
SspBI 198 TGTACA T^GTACA
768 TGTACA
XmnI 345 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AfaI AflIII AluI AlwI BbvI
BfaI BmyI BsaAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII