##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
AscI AseI AsnI BamHI BbeI
BbrPI BglII BsaAI BsiEI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI MfeI
MluI MunI NarI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Van91I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 3357 TCCGGA T^CCGGA
AcsI 3044 AAATTT [GA]^AATT[CT]
AflII 3474 CTTAAG C^TTAAG
ApoI 3044 AAATTT [GA]^AATT[CT]
AviII 4355 TGCGCA TGC^GCA
BclI 1836 TGATCA T^GATCA
BfrI 3474 CTTAAG C^TTAAG
BsaWI 3357 TCCGGA [AT]^CCGG[AT]
BseAI 3357 TCCGGA T^CCGGA
Bsp1407I 1558 TGTACA T^GTACA
BspEI 3357 TCCGGA T^CCGGA
BspHI 621 TCATGA T^CATGA
BsrGI 1558 TGTACA T^GTACA
BssSI 2245 CTCGTG CACGAG(-5/-1)
BstAPI 3726 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 191 GGTGACC G^GT[ACGT]ACC
BstPI 191 GGTGACC G^GT[ACGT]ACC
EcoO65I 191 GGTGACC G^GT[ACGT]ACC
FbaI 1836 TGATCA T^GATCA
FspI 4355 TGCGCA TGC^GCA
HincII 1387 GTCAAC GT[CT]^[GA]AC
HindII 1387 GTCAAC GT[CT]^[GA]AC
MroI 3357 TCCGGA T^CCGGA
NdeI 2785 CATATG CA^TATG
NheI 4499 GCTAGC G^CTAGC
PshAI 168 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 621 TCATGA T^CATGA
SbfI 4589 CCTGCAGG CCTGCA^GG
Sse8387I 4589 CCTGCAGG CCTGCA^GG
SspBI 1558 TGTACA T^GTACA
StuI 820 AGGCCT AGG^CCT
SwaI 670 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 872 GGTACC G^GTACC
2365 GGTACC
AflIII 1547 ACATGT A^C[GA][CT]GT
1656 ACATGT
AhdI 2003 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3371 GACTGCGGGTC
ApaI 439 GGGCCC GGGCC^C
1534 GGGCCC
Asp718I 872 GGTACC G^GTACC
2365 GGTACC
AspEI 2003 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3371 GACTGCGGGTC
AvrII 1192 CCTAGG C^CTAGG
3994 CCTAGG
BbsI 1816 GTCTTC GAAGAC(2/6)
2296 GTCTTC
BglI 2405 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3846 GCCATGTTGGC
BlnI 1192 CCTAGG C^CTAGG
3994 CCTAGG
BlpI 2906 GCTGAGC GC^T[ACGT]AGC
4013 GCTGAGC
Bpu1102I 2906 GCTGAGC GC^T[ACGT]AGC
4013 GCTGAGC
BpuAI 1816 GTCTTC GAAGAC(2/6)
2296 GTCTTC
BsaBI 1909 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4481 GATGGGAATC
BsmI 1924 GAATGC GAATGC(1/-1)
4234 GAATGC
BspLU11I 1547 ACATGT A^CATGT
1656 ACATGT
BsrDI 746 GCAATG GCAATG(2/0)
2360 GCAATG
BstXI 548 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4144 CCAGCACTTTGG
BstYI 1606 GGATCT [GA]^GATC[CT]
4216 AGATCC
CelII 2906 GCTGAGC GC^T[ACGT]AGC
4013 GCTGAGC
DraI 671 TTTAAA TTT^AAA
4098 TTTAAA
DraIII 1522 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2023 CACTCAGTG
Eam1105I 2003 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3371 GACTGCGGGTC
EarI 286 CTCTTC CTCTTC(1/4)
1349 GAAGAG
HaeII 44 GGCGCT [GA]GCGC^[CT]
1853 GGCGCT
KpnI 872 GGTACC GGTAC^C
2365 GGTACC
Ksp632I 286 CTCTTC CTCTTC(1/4)
1349 GAAGAG
KspI 69 CCGCGG CCGC^GG
276 CCGCGG
MaeII 2477 ACGT A^CGT
3346 ACGT
MamI 1909 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4481 GATGGGAATC
MflI 1606 GGATCT [GA]^GATC[CT]
4216 AGATCC
PpuMI 827 AGGTCCC [GA]G^G[AT]CC[CT]
2085 AGGTCCT
PvuII 6 CAGCTG CAG^CTG
1554 CAGCTG
SacII 69 CCGCGG CCGC^GG
276 CCGCGG
SmaI 2823 CCCGGG CCC^GGG
3276 CCCGGG
SspI 3066 AATATT AAT^ATT
4548 AATATT
XhoII 1606 GGATCT [GA]^GATC[CT]
4216 AGATCC
XmaCI 2823 CCCGGG C^CCGGG
3276 CCCGGG
XmaI 2823 CCCGGG C^CCGGG
3276 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcgI BciVI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XmnI