################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccI AclI AgeI Aor51HI AscI AseI AsnI BamHI BbeI BbrPI BglII BsaAI BsiEI BsiWI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI MfeI MluI MunI NarI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 3357 TCCGGA T^CCGGA AcsI 3044 AAATTT [GA]^AATT[CT] AflII 3474 CTTAAG C^TTAAG ApoI 3044 AAATTT [GA]^AATT[CT] AviII 4355 TGCGCA TGC^GCA BclI 1836 TGATCA T^GATCA BfrI 3474 CTTAAG C^TTAAG BsaWI 3357 TCCGGA [AT]^CCGG[AT] BseAI 3357 TCCGGA T^CCGGA Bsp1407I 1558 TGTACA T^GTACA BspEI 3357 TCCGGA T^CCGGA BspHI 621 TCATGA T^CATGA BsrGI 1558 TGTACA T^GTACA BssSI 2245 CTCGTG CACGAG(-5/-1) BstAPI 3726 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 191 GGTGACC G^GT[ACGT]ACC BstPI 191 GGTGACC G^GT[ACGT]ACC EcoO65I 191 GGTGACC G^GT[ACGT]ACC FbaI 1836 TGATCA T^GATCA FspI 4355 TGCGCA TGC^GCA HincII 1387 GTCAAC GT[CT]^[GA]AC HindII 1387 GTCAAC GT[CT]^[GA]AC MroI 3357 TCCGGA T^CCGGA NdeI 2785 CATATG CA^TATG NheI 4499 GCTAGC G^CTAGC PshAI 168 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 621 TCATGA T^CATGA SbfI 4589 CCTGCAGG CCTGCA^GG Sse8387I 4589 CCTGCAGG CCTGCA^GG SspBI 1558 TGTACA T^GTACA StuI 820 AGGCCT AGG^CCT SwaI 670 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 872 GGTACC G^GTACC 2365 GGTACC AflIII 1547 ACATGT A^C[GA][CT]GT 1656 ACATGT AhdI 2003 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3371 GACTGCGGGTC ApaI 439 GGGCCC GGGCC^C 1534 GGGCCC Asp718I 872 GGTACC G^GTACC 2365 GGTACC AspEI 2003 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3371 GACTGCGGGTC AvrII 1192 CCTAGG C^CTAGG 3994 CCTAGG BbsI 1816 GTCTTC GAAGAC(2/6) 2296 GTCTTC BglI 2405 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3846 GCCATGTTGGC BlnI 1192 CCTAGG C^CTAGG 3994 CCTAGG BlpI 2906 GCTGAGC GC^T[ACGT]AGC 4013 GCTGAGC Bpu1102I 2906 GCTGAGC GC^T[ACGT]AGC 4013 GCTGAGC BpuAI 1816 GTCTTC GAAGAC(2/6) 2296 GTCTTC BsaBI 1909 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4481 GATGGGAATC BsmI 1924 GAATGC GAATGC(1/-1) 4234 GAATGC BspLU11I 1547 ACATGT A^CATGT 1656 ACATGT BsrDI 746 GCAATG GCAATG(2/0) 2360 GCAATG BstXI 548 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4144 CCAGCACTTTGG BstYI 1606 GGATCT [GA]^GATC[CT] 4216 AGATCC CelII 2906 GCTGAGC GC^T[ACGT]AGC 4013 GCTGAGC DraI 671 TTTAAA TTT^AAA 4098 TTTAAA DraIII 1522 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 2023 CACTCAGTG Eam1105I 2003 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3371 GACTGCGGGTC EarI 286 CTCTTC CTCTTC(1/4) 1349 GAAGAG HaeII 44 GGCGCT [GA]GCGC^[CT] 1853 GGCGCT KpnI 872 GGTACC GGTAC^C 2365 GGTACC Ksp632I 286 CTCTTC CTCTTC(1/4) 1349 GAAGAG KspI 69 CCGCGG CCGC^GG 276 CCGCGG MaeII 2477 ACGT A^CGT 3346 ACGT MamI 1909 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4481 GATGGGAATC MflI 1606 GGATCT [GA]^GATC[CT] 4216 AGATCC PpuMI 827 AGGTCCC [GA]G^G[AT]CC[CT] 2085 AGGTCCT PvuII 6 CAGCTG CAG^CTG 1554 CAGCTG SacII 69 CCGCGG CCGC^GG 276 CCGCGG SmaI 2823 CCCGGG CCC^GGG 3276 CCCGGG SspI 3066 AATATT AAT^ATT 4548 AATATT XhoII 1606 GGATCT [GA]^GATC[CT] 4216 AGATCC XmaCI 2823 CCCGGG C^CCGGG 3276 CCCGGG XmaI 2823 CCCGGG C^CCGGG 3276 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcgI BciVI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XmnI