################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AclI AflII AgeI AscI AseI AsnI BbrPI BclI BfrI BsiWI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRV EcoT22I FbaI FseI HindIII HpaI MfeI MluI MunI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 2958 GGTACC G^GTACC AccI 2570 GTAGAC GT^[AC][GT]AC AhdI 4712 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2652 GTGCAC G^TGCAC ApaLI 2652 GTGCAC G^TGCAC Asp718I 2958 GGTACC G^GTACC AspEI 4712 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2316 TGCGCA TGC^GCA AvrII 5550 CCTAGG C^CTAGG BciVI 97 GGATAC GTATCC(6/5) BlnI 5550 CCTAGG C^CTAGG BsaAI 4529 TACGTG [CT]AC^GT[GA] BsaBI 847 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 3810 CGGCCG CG[GA][CT]^CG BsmI 6659 GAATGC GAATGC(1/-1) BstEII 3307 GGTGACC G^GT[ACGT]ACC BstPI 3307 GGTGACC G^GT[ACGT]ACC DraI 6986 TTTAAA TTT^AAA DrdI 459 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3810 CGGCCG C^GGCCG Eam1105I 4712 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3810 CGGCCG C^GGCCG Eco52I 3810 CGGCCG C^GGCCG EcoO65I 3307 GGTGACC G^GT[ACGT]ACC EcoRI 968 GAATTC G^AATTC FspI 2316 TGCGCA TGC^GCA KpnI 2958 GGTACC GGTAC^C MamI 847 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 1273 GCTAGC G^CTAGC PshAI 3332 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfiI 4738 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 6113 ACTAGT A^CTAGT SspI 6105 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 2573 GACGTC GACGT^C 3272 GACGTC AccIII 2002 TCCGGA T^CCGGA 4702 TCCGGA AocI 5365 CCTAAGG CC^T[ACGT]AGG 6198 CCTGAGG AspI 4613 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4945 GACCTGGTC BbeI 2720 GGCGCC GGCGC^C 4886 GGCGCC BcgI 2053 CGAGGCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4492 CGAAGAGGATGC BseAI 2002 TCCGGA T^CCGGA 4702 TCCGGA Bsp1407I 3018 TGTACA T^GTACA 3978 TGTACA BspEI 2002 TCCGGA T^CCGGA 4702 TCCGGA BsrBI 2426 GAGCGG CCGCTC(-3/-3) 5879 GAGCGG BsrGI 3018 TGTACA T^GTACA 3978 TGTACA BssSI 71 CACGAG CACGAG(-5/-1) 5933 CTCGTG Bsu36I 5365 CCTAAGG CC^T[ACGT]AGG 6198 CCTGAGG DraIII 907 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 2333 CACATCGTG Eco81I 5365 CCTAAGG CC^T[ACGT]AGG 6198 CCTGAGG KasI 2720 GGCGCC G^GCGCC 4886 GGCGCC KspI 313 CCGCGG CCGC^GG 4859 CCGCGG MroI 2002 TCCGGA T^CCGGA 4702 TCCGGA NarI 2720 GGCGCC GG^CGCC 4886 GGCGCC NdeI 2475 CATATG CA^TATG 6164 CATATG PflFI 4613 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4945 GACCTGGTC SacI 2180 GAGCTC GAGCT^C 2670 GAGCTC SacII 313 CCGCGG CCGC^GG 4859 CCGCGG SexAI 996 ACCTGGT A^CC[AT]GGT 4946 ACCTGGT SfoI 2720 GGCGCC GGC^GCC 4886 GGCGCC SspBI 3018 TGTACA T^GTACA 3978 TGTACA Tth111I 4613 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4945 GACCTGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI