##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI BbrPI BclI BfrI BsiWI
BspDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRV EcoT22I FbaI
FseI HindIII HpaI MfeI MluI
MunI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SbfI SfuI SgrAI SnaBI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2958 GGTACC G^GTACC
AccI 2570 GTAGAC GT^[AC][GT]AC
AhdI 4712 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2652 GTGCAC G^TGCAC
ApaLI 2652 GTGCAC G^TGCAC
Asp718I 2958 GGTACC G^GTACC
AspEI 4712 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2316 TGCGCA TGC^GCA
AvrII 5550 CCTAGG C^CTAGG
BciVI 97 GGATAC GTATCC(6/5)
BlnI 5550 CCTAGG C^CTAGG
BsaAI 4529 TACGTG [CT]AC^GT[GA]
BsaBI 847 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3810 CGGCCG CG[GA][CT]^CG
BsmI 6659 GAATGC GAATGC(1/-1)
BstEII 3307 GGTGACC G^GT[ACGT]ACC
BstPI 3307 GGTGACC G^GT[ACGT]ACC
DraI 6986 TTTAAA TTT^AAA
DrdI 459 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3810 CGGCCG C^GGCCG
Eam1105I 4712 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3810 CGGCCG C^GGCCG
Eco52I 3810 CGGCCG C^GGCCG
EcoO65I 3307 GGTGACC G^GT[ACGT]ACC
EcoRI 968 GAATTC G^AATTC
FspI 2316 TGCGCA TGC^GCA
KpnI 2958 GGTACC GGTAC^C
MamI 847 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1273 GCTAGC G^CTAGC
PshAI 3332 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 4738 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 6113 ACTAGT A^CTAGT
SspI 6105 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 2573 GACGTC GACGT^C
3272 GACGTC
AccIII 2002 TCCGGA T^CCGGA
4702 TCCGGA
AocI 5365 CCTAAGG CC^T[ACGT]AGG
6198 CCTGAGG
AspI 4613 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4945 GACCTGGTC
BbeI 2720 GGCGCC GGCGC^C
4886 GGCGCC
BcgI 2053 CGAGGCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4492 CGAAGAGGATGC
BseAI 2002 TCCGGA T^CCGGA
4702 TCCGGA
Bsp1407I 3018 TGTACA T^GTACA
3978 TGTACA
BspEI 2002 TCCGGA T^CCGGA
4702 TCCGGA
BsrBI 2426 GAGCGG CCGCTC(-3/-3)
5879 GAGCGG
BsrGI 3018 TGTACA T^GTACA
3978 TGTACA
BssSI 71 CACGAG CACGAG(-5/-1)
5933 CTCGTG
Bsu36I 5365 CCTAAGG CC^T[ACGT]AGG
6198 CCTGAGG
DraIII 907 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
2333 CACATCGTG
Eco81I 5365 CCTAAGG CC^T[ACGT]AGG
6198 CCTGAGG
KasI 2720 GGCGCC G^GCGCC
4886 GGCGCC
KspI 313 CCGCGG CCGC^GG
4859 CCGCGG
MroI 2002 TCCGGA T^CCGGA
4702 TCCGGA
NarI 2720 GGCGCC GG^CGCC
4886 GGCGCC
NdeI 2475 CATATG CA^TATG
6164 CATATG
PflFI 4613 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4945 GACCTGGTC
SacI 2180 GAGCTC GAGCT^C
2670 GAGCTC
SacII 313 CCGCGG CCGC^GG
4859 CCGCGG
SexAI 996 ACCTGGT A^CC[AT]GGT
4946 ACCTGGT
SfoI 2720 GGCGCC GGC^GCC
4886 GGCGCC
SspBI 3018 TGTACA T^GTACA
3978 TGTACA
Tth111I 4613 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4945 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI