##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
BclI BglII BsiWI BspDI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI EcoRV
FbaI FseI FspI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfiI SfuI
SgrAI SpeI Sse8387I SspI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 1025 TCCGGA T^CCGGA
AcsI 4639 GAATTT [GA]^AATT[CT]
AflII 3214 CTTAAG C^TTAAG
AgeI 4631 ACCGGT A^CCGGT
Alw44I 2240 GTGCAC G^TGCAC
ApaLI 2240 GTGCAC G^TGCAC
ApoI 4639 GAATTT [GA]^AATT[CT]
BbeI 6174 GGCGCC GGCGC^C
BcgI 2841 CGACACTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3243 GTATCC GTATCC(6/5)
BfrI 3214 CTTAAG C^TTAAG
BglI 6166 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3227 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1025 TCCGGA T^CCGGA
BsiEI 409 CGGCCG CG[GA][CT]^CG
BsmI 5237 GCATTC GAATGC(1/-1)
Bsp1407I 618 TGTACA T^GTACA
BspEI 1025 TCCGGA T^CCGGA
BsrGI 618 TGTACA T^GTACA
BssSI 5867 CACGAG CACGAG(-5/-1)
EagI 409 CGGCCG C^GGCCG
EclXI 409 CGGCCG C^GGCCG
Eco52I 409 CGGCCG C^GGCCG
EcoT22I 5235 ATGCAT ATGCA^T
HincII 4854 GTTAAC GT[CT]^[GA]AC
HindII 4854 GTTAAC GT[CT]^[GA]AC
HpaI 4854 GTTAAC GTT^AAC
KasI 6174 GGCGCC G^GCGCC
MamI 3227 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1025 TCCGGA T^CCGGA
NaeI 411 GCCGGC GCC^GGC
NarI 6174 GGCGCC GG^CGCC
NgoMIV 411 GCCGGC G^CCGGC
NsiI 5235 ATGCAT ATGCA^T
PinAI 4631 ACCGGT A^CCGGT
SexAI 1936 ACCTGGT A^CC[AT]GGT
SfoI 6174 GGCGCC GGC^GCC
SnaBI 297 TACGTA TAC^GTA
SspBI 618 TGTACA T^GTACA
SwaI 283 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflIII 615 ACGTGT A^C[GA][CT]GT
649 ACGTGT
BbrPI 614 CACGTG CAC^GTG
911 CACGTG
DraI 284 TTTAAA TTT^AAA
1445 TTTAAA
DraIII 1935 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5112 CACAGCGTG
DrdI 1854 GACGCCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5159 GACTCAGGAGTC
HindIII 2940 AAGCTT A^AGCTT
5294 AAGCTT
KspI 72 CCGCGG CCGC^GG
120 CCGCGG
PmaCI 614 CACGTG CAC^GTG
911 CACGTG
PmlI 614 CACGTG CAC^GTG
911 CACGTG
SacII 72 CCGCGG CCGC^GG
120 CCGCGG
ScaI 2486 AGTACT AGT^ACT
5971 AGTACT
SphI 415 GCATGC GCATG^C
6089 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AspI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI