################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccII AccIII AhdI Alw44I AocI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BbeI BbvCI BglI BlnI BlpI Bpu1102I BseAI BsiWI BsmBI BsoBI BspEI BsrBI BssHII BstAPI BstBI BstUI Bsu36I CelII CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI FseI FspI KasI KpnI KspI MluI MroI MvnI NarI NcoI NotI NruI NspV PacI PaeR7I PmeI PpuMI PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SmaI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 3381 GACGTC GACGT^C AclI 6672 AACGTT AA^CGTT AflII 1918 CTTAAG C^TTAAG AgeI 2202 ACCGGT A^CCGGT Aor51HI 860 AGCGCT AGC^GCT ApaI 2772 GGGCCC GGGCC^C AspI 86 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 7013 TGGCCA TGG^CCA BamHI 6589 GGATCC G^GATCC BanI 3238 GGTGCC G^G[CT][GA]CC BbrPI 6081 CACGTG CAC^GTG BcgI 5527 GCAACCCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5880 GTATCC GTATCC(6/5) BfrI 1918 CTTAAG C^TTAAG BsaBI 308 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2202 ACCGGT [AT]^CCGG[AT] BsiEI 5533 CGGTCG CG[GA][CT]^CG BspDI 5066 ATCGAT AT^CGAT BssSI 3536 CTCGTG CACGAG(-5/-1) BstEII 1765 GGTGACC G^GT[ACGT]ACC BstPI 1765 GGTGACC G^GT[ACGT]ACC ClaI 5066 ATCGAT AT^CGAT DraIII 2891 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 7013 TGGCCA [CT]^GGCC[GA] Eco47III 860 AGCGCT AGC^GCT EcoO65I 1765 GGTGACC G^GT[ACGT]ACC EcoT22I 3281 ATGCAT ATGCA^T FauI 2769 GCGGG CCCGC(4/6) HaeII 860 AGCGCT [GA]GCGC^[CT] HgaI 853 GACGC GACGC(5/10) HindIII 1100 AAGCTT A^AGCTT HpaI 3802 GTTAAC GTT^AAC MamI 308 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4546 CAATTG C^AATTG MluNI 7013 TGGCCA TGG^CCA MscI 7013 TGGCCA TGG^CCA MunI 4546 CAATTG C^AATTG NaeI 4716 GCCGGC GCC^GGC NdeI 441 CATATG CA^TATG NgoMIV 4716 GCCGGC G^CCGGC NheI 4749 GCTAGC G^CTAGC NsiI 3281 ATGCAT ATGCA^T PflFI 86 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2202 ACCGGT A^CCGGT PmaCI 6081 CACGTG CAC^GTG PmlI 6081 CACGTG CAC^GTG Psp1406I 6672 AACGTT AA^CGTT SexAI 7200 ACCAGGT A^CC[AT]GGT SgrAI 2201 CACCGGTG C[GA]^CCGG[CT]G SnaBI 1140 TACGTA TAC^GTA SphI 6956 GCATGC GCATG^C SwaI 3581 ATTTAAAT ATTT^AAAT Tth111I 86 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5433 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 853 GACGCC G[GA]^CG[CT]C 3381 GACGTC AvaII 1468 GGACC G^G[AT]CC 3556 GGTCC BcnI 4617 CCGGG CC^[GC]GG 5531 CCCGG BsaAI 1140 TACGTA [CT]AC^GT[GA] 6081 CACGTG BsaHI 853 GACGCC G[GA]^CG[CT]C 3381 GACGTC BsaI 4807 GAGACC GGTCTC(1/5) 6226 GAGACC BspLU11I 4582 ACATGT A^CATGT 7844 ACATGT BsrDI 7618 CATTGC GCAATG(2/0) 6098 GCAATG BsrFI 2202 ACCGGT [GA]^CCGG[CT] 4716 GCCGGC Bst1107I 980 GTATAC GTA^TAC 5488 GTATAC BstZ17I 980 GTATAC GTA^TAC 5488 GTATAC CfoI 861 GCGC GCG^C 2124 GCGC Cfr10I 2202 ACCGGT [GA]^CCGG[CT] 4716 GCCGGC DraII 2771 GGGGCCC [GA]G^G[ACGT]CC[CT] 8639 AGGGCCT DsaI 2763 CCGTGG C^C[GA][CT]GG 5445 CCACGG EcoO109I 2771 GGGGCCC [GA]G^G[ACGT]CC[CT] 8639 AGGGCCT EcoT14I 378 CCTTGG C^C[AT][AT]GG 4443 CCAAGG HhaI 861 GCGC GCG^C 2124 GCGC Hin1I 853 GACGCC G[GA]^CG[CT]C 3381 GACGTC HinP1I 861 GCGC G^CGC 2124 GCGC NciI 4617 CCGGG CC^[GC]GG 5531 CCCGG PshAI 1469 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 7044 GACATTTGTC SacI 1450 GAGCTC GAGCT^C 6474 GAGCTC SpeI 6634 ACTAGT A^CTAGT 7748 ACTAGT StuI 556 AGGCCT AGG^CCT 4681 AGGCCT StyI 378 CCTTGG C^C[AT][AT]GG 4443 CCAAGG XcmI 5274 CCAAGCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6564 CCATCCGAATACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI BanII BbsI BbvI BclI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRI EcoRII EcoRV FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI