##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccII AccIII AhdI Alw44I
AocI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BbeI BbvCI
BglI BlnI BlpI Bpu1102I BseAI
BsiWI BsmBI BsoBI BspEI BsrBI
BssHII BstAPI BstBI BstUI Bsu36I
CelII CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI FseI
FspI KasI KpnI KspI MluI
MroI MvnI NarI NcoI NotI
NruI NspV PacI PaeR7I PmeI
PpuMI PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SmaI Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 3381 GACGTC GACGT^C
AclI 6672 AACGTT AA^CGTT
AflII 1918 CTTAAG C^TTAAG
AgeI 2202 ACCGGT A^CCGGT
Aor51HI 860 AGCGCT AGC^GCT
ApaI 2772 GGGCCC GGGCC^C
AspI 86 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 7013 TGGCCA TGG^CCA
BamHI 6589 GGATCC G^GATCC
BanI 3238 GGTGCC G^G[CT][GA]CC
BbrPI 6081 CACGTG CAC^GTG
BcgI 5527 GCAACCCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5880 GTATCC GTATCC(6/5)
BfrI 1918 CTTAAG C^TTAAG
BsaBI 308 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2202 ACCGGT [AT]^CCGG[AT]
BsiEI 5533 CGGTCG CG[GA][CT]^CG
BspDI 5066 ATCGAT AT^CGAT
BssSI 3536 CTCGTG CACGAG(-5/-1)
BstEII 1765 GGTGACC G^GT[ACGT]ACC
BstPI 1765 GGTGACC G^GT[ACGT]ACC
ClaI 5066 ATCGAT AT^CGAT
DraIII 2891 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 7013 TGGCCA [CT]^GGCC[GA]
Eco47III 860 AGCGCT AGC^GCT
EcoO65I 1765 GGTGACC G^GT[ACGT]ACC
EcoT22I 3281 ATGCAT ATGCA^T
FauI 2769 GCGGG CCCGC(4/6)
HaeII 860 AGCGCT [GA]GCGC^[CT]
HgaI 853 GACGC GACGC(5/10)
HindIII 1100 AAGCTT A^AGCTT
HpaI 3802 GTTAAC GTT^AAC
MamI 308 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4546 CAATTG C^AATTG
MluNI 7013 TGGCCA TGG^CCA
MscI 7013 TGGCCA TGG^CCA
MunI 4546 CAATTG C^AATTG
NaeI 4716 GCCGGC GCC^GGC
NdeI 441 CATATG CA^TATG
NgoMIV 4716 GCCGGC G^CCGGC
NheI 4749 GCTAGC G^CTAGC
NsiI 3281 ATGCAT ATGCA^T
PflFI 86 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2202 ACCGGT A^CCGGT
PmaCI 6081 CACGTG CAC^GTG
PmlI 6081 CACGTG CAC^GTG
Psp1406I 6672 AACGTT AA^CGTT
SexAI 7200 ACCAGGT A^CC[AT]GGT
SgrAI 2201 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 1140 TACGTA TAC^GTA
SphI 6956 GCATGC GCATG^C
SwaI 3581 ATTTAAAT ATTT^AAAT
Tth111I 86 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5433 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 853 GACGCC G[GA]^CG[CT]C
3381 GACGTC
AvaII 1468 GGACC G^G[AT]CC
3556 GGTCC
BcnI 4617 CCGGG CC^[GC]GG
5531 CCCGG
BsaAI 1140 TACGTA [CT]AC^GT[GA]
6081 CACGTG
BsaHI 853 GACGCC G[GA]^CG[CT]C
3381 GACGTC
BsaI 4807 GAGACC GGTCTC(1/5)
6226 GAGACC
BspLU11I 4582 ACATGT A^CATGT
7844 ACATGT
BsrDI 7618 CATTGC GCAATG(2/0)
6098 GCAATG
BsrFI 2202 ACCGGT [GA]^CCGG[CT]
4716 GCCGGC
Bst1107I 980 GTATAC GTA^TAC
5488 GTATAC
BstZ17I 980 GTATAC GTA^TAC
5488 GTATAC
CfoI 861 GCGC GCG^C
2124 GCGC
Cfr10I 2202 ACCGGT [GA]^CCGG[CT]
4716 GCCGGC
DraII 2771 GGGGCCC [GA]G^G[ACGT]CC[CT]
8639 AGGGCCT
DsaI 2763 CCGTGG C^C[GA][CT]GG
5445 CCACGG
EcoO109I 2771 GGGGCCC [GA]G^G[ACGT]CC[CT]
8639 AGGGCCT
EcoT14I 378 CCTTGG C^C[AT][AT]GG
4443 CCAAGG
HhaI 861 GCGC GCG^C
2124 GCGC
Hin1I 853 GACGCC G[GA]^CG[CT]C
3381 GACGTC
HinP1I 861 GCGC G^CGC
2124 GCGC
NciI 4617 CCGGG CC^[GC]GG
5531 CCCGG
PshAI 1469 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7044 GACATTTGTC
SacI 1450 GAGCTC GAGCT^C
6474 GAGCTC
SpeI 6634 ACTAGT A^CTAGT
7748 ACTAGT
StuI 556 AGGCCT AGG^CCT
4681 AGGCCT
StyI 378 CCTTGG C^C[AT][AT]GG
4443 CCAAGG
XcmI 5274 CCAAGCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6564 CCATCCGAATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI BanII BbsI
BbvI BclI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoRV FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI