##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AhdI Aor51HI
AseI AsnI AspEI AviII BamHI
BbrPI BsaBI BseAI BsiWI BspDI
BspEI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FspI HpaI MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 5253 GGTACC G^GTACC
AccI 1977 GTCGAC GT^[AC][GT]AC
AflII 667 CTTAAG C^TTAAG
AgeI 2415 ACCGGT A^CCGGT
AscI 1620 GGCGCGCC GG^CGCGCC
Asp718I 5253 GGTACC G^GTACC
AspI 1031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 381 GGCGCC GGCGC^C
BcgI 341 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5901 TGATCA T^GATCA
BfrI 667 CTTAAG C^TTAAG
BsaAI 4648 CACGTA [CT]AC^GT[GA]
BsiEI 2796 CGGTCG CG[GA][CT]^CG
BsmBI 2322 CGTCTC CGTCTC(1/5)
BspHI 5904 TCATGA T^CATGA
BsrBI 4474 GAGCGG CCGCTC(-3/-3)
BstAPI 2721 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 2836 CGGACCG CG^G[AT]CCG
FbaI 5901 TGATCA T^GATCA
FseI 4055 GGCCGGCC GGCCGG^CC
HgaI 6183 GCGTC GACGC(5/10)
KasI 381 GGCGCC G^GCGCC
KpnI 5253 GGTACC GGTAC^C
KspI 2840 CCGCGG CCGC^GG
NaeI 4056 GCCGGC GCC^GGC
NarI 381 GGCGCC GG^CGCC
NgoMIV 4056 GCCGGC G^CCGGC
PflFI 1031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2415 ACCGGT A^CCGGT
RcaI 5904 TCATGA T^CATGA
RsrII 2836 CGGACCG CG^G[AT]CCG
SacII 2840 CCGCGG CCGC^GG
SalI 1977 GTCGAC G^TCGAC
SfiI 1894 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 381 GGCGCC GGC^GCC
SmaI 1898 CCCGGG CCC^GGG
SspI 5916 AATATT AAT^ATT
SwaI 6011 ATTTAAAT ATTT^AAAT
Tth111I 1031 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5196 TCTAGA T^CTAGA
XmaCI 1898 CCCGGG C^CCGGG
XmaI 1898 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 381 GGCGCC G[GA]^CG[CT]C
6182 GGCGTC
AflIII 3851 ACATGT A^C[GA][CT]GT
4216 ACATGT
Alw44I 2666 GTGCAC G^TGCAC
3894 GTGCAC
ApaI 1902 GGGCCC GGGCC^C
2109 GGGCCC
ApaLI 2666 GTGCAC G^TGCAC
3894 GTGCAC
BciVI 5929 GGATAC GTATCC(6/5)
3805 GTATCC
BglII 776 AGATCT A^GATCT
4944 AGATCT
BlpI 2627 GCTCAGC GC^T[ACGT]AGC
6286 GCTGAGC
Bpu1102I 2627 GCTCAGC GC^T[ACGT]AGC
6286 GCTGAGC
BsaHI 381 GGCGCC G[GA]^CG[CT]C
6182 GGCGTC
BsaWI 2415 ACCGGT [AT]^CCGG[AT]
5051 ACCGGA
Bsp1407I 6144 TGTACA T^GTACA
6408 TGTACA
BspLU11I 3851 ACATGT A^CATGT
4216 ACATGT
BsrDI 662 CATTGC GCAATG(2/0)
5543 GCAATG
BsrGI 6144 TGTACA T^GTACA
6408 TGTACA
CelII 2627 GCTCAGC GC^T[ACGT]AGC
6286 GCTGAGC
EcoNI 1672 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1870 CCTGTGGAAGG
EcoRI 2451 GAATTC G^AATTC
4006 GAATTC
HaeII 381 GGCGCC [GA]GCGC^[CT]
2683 AGCGCC
Hin1I 381 GGCGCC G[GA]^CG[CT]C
6182 GGCGTC
HindIII 4606 AAGCTT A^AGCTT
5018 AAGCTT
NdeI 654 CATATG CA^TATG
3776 CATATG
PaeR7I 1950 CTCGAG C^TCGAG
2072 CTCGAG
SapI 1332 GCTCTTC GCTCTTC(1/4)
1357 GCTCTTC
SspBI 6144 TGTACA T^GTACA
6408 TGTACA
XhoI 1950 CTCGAG C^TCGAG
2072 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI