##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI Aor51HI AscI
AviII BbeI BcgI BglII BlpI
Bpu1102I BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstPI
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
FspI HaeII HpaI KasI KspI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 5030 GGTACC G^GTACC
AccII 1942 CGCG CG^CG
AcyI 2783 GGCGTC G[GA]^CG[CT]C
AgeI 6935 ACCGGT A^CCGGT
AhdI 2253 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6806 GTGCAC G^TGCAC
AlwNI 3051 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 404 CCTGAGG CC^T[ACGT]AGG
ApaLI 6806 GTGCAC G^TGCAC
Asp718I 5030 GGTACC G^GTACC
AspEI 2253 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3100 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1839 CCTAGG C^CTAGG
BalI 2145 TGGCCA TGG^CCA
BamHI 4405 GGATCC G^GATCC
BbrPI 3523 CACGTG CAC^GTG
BbvCI 1911 GCTGAGG CCTCAGC(-5/-2)
BglI 3261 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1839 CCTAGG C^CTAGG
BsaBI 1366 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2783 GGCGTC G[GA]^CG[CT]C
BsaWI 6935 ACCGGT [AT]^CCGG[AT]
BsrFI 6935 ACCGGT [GA]^CCGG[CT]
Bst1107I 3406 GTATAC GTA^TAC
BstUI 1942 CGCG CG^CG
BstXI 6089 CCATACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3406 GTATAC GTA^TAC
Bsu36I 404 CCTGAGG CC^T[ACGT]AGG
CfoI 3287 GCGC GCG^C
Cfr10I 6935 ACCGGT [GA]^CCGG[CT]
DrdI 990 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 6095 CCATGG C^C[GA][CT]GG
EaeI 2145 TGGCCA [CT]^GGCC[GA]
Eam1105I 2253 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 404 CCTGAGG CC^T[ACGT]AGG
FauI 6124 GCGGG CCCGC(4/6)
HhaI 3287 GCGC GCG^C
Hin1I 2783 GGCGTC G[GA]^CG[CT]C
HinP1I 3287 GCGC G^CGC
HincII 2525 GTTGAC GT[CT]^[GA]AC
HindII 2525 GTTGAC GT[CT]^[GA]AC
KpnI 5030 GGTACC GGTAC^C
MamI 1366 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1941 ACGCGT A^CGCGT
MluNI 2145 TGGCCA TGG^CCA
MscI 2145 TGGCCA TGG^CCA
MvnI 1942 CGCG CG^CG
NcoI 6095 CCATGG C^CATGG
NheI 248 GCTAGC G^CTAGC
PflFI 3100 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 6935 ACCGGT A^CCGGT
PmaCI 3523 CACGTG CAC^GTG
PmlI 3523 CACGTG CAC^GTG
PpuMI 4012 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 6560 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 5233 CAGCTG CAG^CTG
SapI 3275 GCTCTTC GCTCTTC(1/4)
ScaI 2945 AGTACT AGT^ACT
SexAI 5682 ACCAGGT A^CC[AT]GGT
SmaI 2919 CCCGGG CCC^GGG
SpeI 222 ACTAGT A^CTAGT
StuI 4795 AGGCCT AGG^CCT
SwaI 971 ATTTAAAT ATTT^AAAT
Tth111I 3100 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5057 TCTAGA T^CTAGA
XmaCI 2919 CCCGGG C^CCGGG
XmaI 2919 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 1539 CTTAAG C^TTAAG
4262 CTTAAG
ApaI 4116 GGGCCC GGGCC^C
6424 GGGCCC
AspHI 793 GAGCAC G[AT]GC[AT]^C
6806 GTGCAC
AvaI 2919 CCCGGG C^[CT]CG[GA]G
6605 CCCGAG
BbsI 4669 GTCTTC GAAGAC(2/6)
5837 GTCTTC
BclI 2354 TGATCA T^GATCA
5468 TGATCA
BcnI 2919 CCCGG CC^[GC]GG
2920 CCGGG
BfrI 1539 CTTAAG C^TTAAG
4262 CTTAAG
BpuAI 4669 GTCTTC GAAGAC(2/6)
5837 GTCTTC
BsiHKAI 793 GAGCAC G[AT]GC[AT]^C
6806 GTGCAC
BsoBI 2919 CCCGGG C^[CT]CG[GA]G
6605 CCCGAG
BspMI 2959 ACCTGC ACCTGC(4/8)
2313 GCAGGT
BstYI 2668 GGATCT [GA]^GATC[CT]
4405 GGATCC
DraII 4012 GGGACCT [GA]G^G[ACGT]CC[CT]
6424 GGGCCCC
DraIII 4599 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5544 CACAAGGTG
EcoO109I 4012 GGGACCT [GA]G^G[ACGT]CC[CT]
6424 GGGCCCC
EcoRI 4160 GAATTC G^AATTC
4322 GAATTC
EcoT14I 1839 CCTAGG C^C[AT][AT]GG
6095 CCATGG
FbaI 2354 TGATCA T^GATCA
5468 TGATCA
HapII 2920 CCGG C^CGG
6936 CCGG
HgaI 1943 GCGTC GACGC(5/10)
2784 GCGTC
HindIII 192 AAGCTT A^AGCTT
6658 AAGCTT
HpaII 2920 CCGG C^CGG
6936 CCGG
MflI 2668 GGATCT [GA]^GATC[CT]
4405 GGATCC
MspA1I 5233 CAGCTG C[AC]G^C[GT]G
5516 CCGCTG
MspI 2920 CCGG C^CGG
6936 CCGG
NciI 2919 CCCGG CC^[GC]GG
2920 CCGGG
NdeI 3411 CATATG CA^TATG
3700 CATATG
PflMI 515 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4240 CCAGTGTGTGG
SnaBI 4879 TACGTA TAC^GTA
6888 TACGTA
SphI 832 GCATGC GCATG^C
1922 GCATGC
StyI 1839 CCTAGG C^C[AT][AT]GG
6095 CCATGG
Van91I 515 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4240 CCAGTGTGTGG
XhoII 2668 GGATCT [GA]^GATC[CT]
4405 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AvaII BanI BanII BbvI
BciVI BfaI BmyI BpmI BsaAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoRV EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MnlI MseI MslI MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmnI