################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AccIII AflII AgeI Alw44I ApaLI AscI AviII BcgI BfrI BsaBI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraIII EcoO65I EcoRV FseI FspI MamI MfeI MluI MroI MunI NaeI NgoMIV NotI NruI PacI PinAI PmeI PvuI RsrII SalI SbfI SfiI SmaI SnaBI SpeI Sse8387I StuI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 715 GACGTC GACGT^C AccI 2377 GTAGAC GT^[AC][GT]AC AclI 1830 AACGTT AA^CGTT AhdI 1635 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 2785 GGGCCC GGGCC^C AseI 3895 ATTAAT AT^TAAT AsnI 3895 ATTAAT AT^TAAT AspEI 1635 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1145 TGGCCA TGG^CCA BamHI 250 GGATCC G^GATCC BbeI 168 GGCGCC GGCGC^C BbsI 1179 GAAGAC GAAGAC(2/6) BlpI 1114 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1114 GCTGAGC GC^T[ACGT]AGC BpuAI 1179 GAAGAC GAAGAC(2/6) BsiEI 106 CGGCCG CG[GA][CT]^CG BsmBI 4489 GAGACG CGTCTC(1/5) BspLU11I 3100 ACATGT A^CATGT BspMI 5716 GCAGGT ACCTGC(4/8) BstAPI 5917 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 309 TTCGAA TT^CGAA CelII 1114 GCTGAGC GC^T[ACGT]AGC DrdI 709 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 106 CGGCCG C^GGCCG Eam1105I 1635 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 106 CGGCCG C^GGCCG Eco52I 106 CGGCCG C^GGCCG EcoRI 5012 GAATTC G^AATTC KasI 168 GGCGCC G^GCGCC MluNI 1145 TGGCCA TGG^CCA MscI 1145 TGGCCA TGG^CCA NarI 168 GGCGCC GG^CGCC NcoI 5547 CCATGG C^CATGG NdeI 419 CATATG CA^TATG NheI 954 GCTAGC G^CTAGC NspV 309 TTCGAA TT^CGAA PshAI 5147 GACGTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3895 ATTAAT AT^TAAT Psp1406I 1830 AACGTT AA^CGTT SexAI 4562 ACCAGGT A^CC[AT]GGT SfoI 168 GGCGCC GGC^GCC SfuI 309 TTCGAA TT^CGAA SgrAI 164 CACCGGCG C[GA]^CCGG[CT]G SspI 1878 AATATT AAT^ATT SwaI 3596 ATTTAAAT ATTT^AAAT XbaI 2456 TCTAGA T^CTAGA XcmI 765 CCAGACATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 668 GGTACC G^GTACC 760 GGTACC Asp700I 546 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1703 GAATAGTTTC Asp718I 668 GGTACC G^GTACC 760 GGTACC AspI 4491 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6059 GACCCTGTC AvrII 1598 CCTAGG C^CTAGG 6197 CCTAGG BclI 1415 TGATCA T^GATCA 5137 TGATCA BglI 2778 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6196 GCCTAGGTGGC BlnI 1598 CCTAGG C^CTAGG 6197 CCTAGG BsgI 6186 CTGCAC GTGCAG(16/14) 3651 GTGCAG BsmI 414 GAATGC GAATGC(1/-1) 3819 GCATTC Bsp1407I 2884 TGTACA T^GTACA 3097 TGTACA BspHI 4081 TCATGA T^CATGA 5008 TCATGA BsrDI 4239 CATTGC GCAATG(2/0) 4834 CATTGC BsrFI 165 ACCGGC [GA]^CCGG[CT] 2593 GCCGGT BsrGI 2884 TGTACA T^GTACA 3097 TGTACA Cfr10I 165 ACCGGC [GA]^CCGG[CT] 2593 GCCGGT EaeI 106 CGGCCG [CT]^GGCC[GA] 1145 TGGCCA FbaI 1415 TGATCA T^GATCA 5137 TGATCA HpaI 3904 GTTAAC GTT^AAC 5078 GTTAAC KpnI 668 GGTACC GGTAC^C 760 GGTACC KspI 8 CCGCGG CCGC^GG 172 CCGCGG PaeR7I 1297 CTCGAG C^TCGAG 2711 CTCGAG PflFI 4491 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6059 GACCCTGTC PflMI 4792 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5833 CCAGACACTGG RcaI 4081 TCATGA T^CATGA 5008 TCATGA SacII 8 CCGCGG CCGC^GG 172 CCGCGG ScaI 1463 AGTACT AGT^ACT 4327 AGTACT SphI 915 GCATGC GCATG^C 6107 GCATGC SspBI 2884 TGTACA T^GTACA 3097 TGTACA Tth111I 4491 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 6059 GACCCTGTC Van91I 4792 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5833 CCAGACACTGG XhoI 1297 CTCGAG C^TCGAG 2711 CTCGAG XmnI 546 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1703 GAATAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI Aor51HI ApoI AspHI AvaI AvaII BanI BanII BbrPI BbvCI BbvI BciVI BcnI BfaI BglII BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII