##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AflII AgeI Alw44I ApaLI
AscI AviII BcgI BfrI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
EcoO65I EcoRV FseI FspI MamI
MfeI MluI MroI MunI NaeI
NgoMIV NotI NruI PacI PinAI
PmeI PvuI RsrII SalI SbfI
SfiI SmaI SnaBI SpeI Sse8387I
StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 715 GACGTC GACGT^C
AccI 2377 GTAGAC GT^[AC][GT]AC
AclI 1830 AACGTT AA^CGTT
AhdI 1635 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2785 GGGCCC GGGCC^C
AseI 3895 ATTAAT AT^TAAT
AsnI 3895 ATTAAT AT^TAAT
AspEI 1635 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1145 TGGCCA TGG^CCA
BamHI 250 GGATCC G^GATCC
BbeI 168 GGCGCC GGCGC^C
BbsI 1179 GAAGAC GAAGAC(2/6)
BlpI 1114 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1114 GCTGAGC GC^T[ACGT]AGC
BpuAI 1179 GAAGAC GAAGAC(2/6)
BsiEI 106 CGGCCG CG[GA][CT]^CG
BsmBI 4489 GAGACG CGTCTC(1/5)
BspLU11I 3100 ACATGT A^CATGT
BspMI 5716 GCAGGT ACCTGC(4/8)
BstAPI 5917 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 309 TTCGAA TT^CGAA
CelII 1114 GCTGAGC GC^T[ACGT]AGC
DrdI 709 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 106 CGGCCG C^GGCCG
Eam1105I 1635 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 106 CGGCCG C^GGCCG
Eco52I 106 CGGCCG C^GGCCG
EcoRI 5012 GAATTC G^AATTC
KasI 168 GGCGCC G^GCGCC
MluNI 1145 TGGCCA TGG^CCA
MscI 1145 TGGCCA TGG^CCA
NarI 168 GGCGCC GG^CGCC
NcoI 5547 CCATGG C^CATGG
NdeI 419 CATATG CA^TATG
NheI 954 GCTAGC G^CTAGC
NspV 309 TTCGAA TT^CGAA
PshAI 5147 GACGTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3895 ATTAAT AT^TAAT
Psp1406I 1830 AACGTT AA^CGTT
SexAI 4562 ACCAGGT A^CC[AT]GGT
SfoI 168 GGCGCC GGC^GCC
SfuI 309 TTCGAA TT^CGAA
SgrAI 164 CACCGGCG C[GA]^CCGG[CT]G
SspI 1878 AATATT AAT^ATT
SwaI 3596 ATTTAAAT ATTT^AAAT
XbaI 2456 TCTAGA T^CTAGA
XcmI 765 CCAGACATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 668 GGTACC G^GTACC
760 GGTACC
Asp700I 546 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1703 GAATAGTTTC
Asp718I 668 GGTACC G^GTACC
760 GGTACC
AspI 4491 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6059 GACCCTGTC
AvrII 1598 CCTAGG C^CTAGG
6197 CCTAGG
BclI 1415 TGATCA T^GATCA
5137 TGATCA
BglI 2778 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6196 GCCTAGGTGGC
BlnI 1598 CCTAGG C^CTAGG
6197 CCTAGG
BsgI 6186 CTGCAC GTGCAG(16/14)
3651 GTGCAG
BsmI 414 GAATGC GAATGC(1/-1)
3819 GCATTC
Bsp1407I 2884 TGTACA T^GTACA
3097 TGTACA
BspHI 4081 TCATGA T^CATGA
5008 TCATGA
BsrDI 4239 CATTGC GCAATG(2/0)
4834 CATTGC
BsrFI 165 ACCGGC [GA]^CCGG[CT]
2593 GCCGGT
BsrGI 2884 TGTACA T^GTACA
3097 TGTACA
Cfr10I 165 ACCGGC [GA]^CCGG[CT]
2593 GCCGGT
EaeI 106 CGGCCG [CT]^GGCC[GA]
1145 TGGCCA
FbaI 1415 TGATCA T^GATCA
5137 TGATCA
HpaI 3904 GTTAAC GTT^AAC
5078 GTTAAC
KpnI 668 GGTACC GGTAC^C
760 GGTACC
KspI 8 CCGCGG CCGC^GG
172 CCGCGG
PaeR7I 1297 CTCGAG C^TCGAG
2711 CTCGAG
PflFI 4491 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6059 GACCCTGTC
PflMI 4792 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5833 CCAGACACTGG
RcaI 4081 TCATGA T^CATGA
5008 TCATGA
SacII 8 CCGCGG CCGC^GG
172 CCGCGG
ScaI 1463 AGTACT AGT^ACT
4327 AGTACT
SphI 915 GCATGC GCATG^C
6107 GCATGC
SspBI 2884 TGTACA T^GTACA
3097 TGTACA
Tth111I 4491 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6059 GACCCTGTC
Van91I 4792 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5833 CCAGACACTGG
XhoI 1297 CTCGAG C^TCGAG
2711 CTCGAG
XmnI 546 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1703 GAATAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApoI AspHI AvaI AvaII
BanI BanII BbrPI BbvCI BbvI
BciVI BcnI BfaI BglII BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII