##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BclI BcnI BglII BlnI BsaI
BsiEI BsiWI BsmBI BspDI BspLU11I
BsrBI BsrFI BssHII BssKI BstAPI
BstBI BstNI BstUI Bsu36I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRII EcoRV FbaI FseI FspI
KspI MluI MvaI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI RsrII SacII SapI SbfI
ScaI ScrFI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 74 enzymes) #
###################################
Acc65I 1830 GGTACC G^GTACC
AccIII 1026 TCCGGA T^CCGGA
AclI 974 AACGTT AA^CGTT
AcyI 472 GGCGCC G[GA]^CG[CT]C
AflII 2113 CTTAAG C^TTAAG
AseI 2014 ATTAAT AT^TAAT
AsnI 2014 ATTAAT AT^TAAT
Asp718I 1830 GGTACC G^GTACC
AvaI 602 CTCGAG C^[CT]CG[GA]G
BalI 93 TGGCCA TGG^CCA
BanII 857 GAGCTC G[GA]GC[CT]^C
BbeI 472 GGCGCC GGCGC^C
BbsI 910 GAAGAC GAAGAC(2/6)
BfrI 2113 CTTAAG C^TTAAG
BglI 1532 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 343 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 343 GCTGAGC GC^T[ACGT]AGC
BpuAI 910 GAAGAC GAAGAC(2/6)
BsaAI 615 CACGTA [CT]AC^GT[GA]
BsaHI 472 GGCGCC G[GA]^CG[CT]C
BsaWI 1026 TCCGGA [AT]^CCGG[AT]
BseAI 1026 TCCGGA T^CCGGA
BsgI 1286 CTGCAC GTGCAG(16/14)
BsmI 70 GAATGC GAATGC(1/-1)
BsoBI 602 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1520 TGTACA T^GTACA
BspEI 1026 TCCGGA T^CCGGA
BsrDI 1973 GCAATG GCAATG(2/0)
BsrGI 1520 TGTACA T^GTACA
BssSI 1036 CTCGTG CACGAG(-5/-1)
BstEII 1748 GGTCACC G^GT[ACGT]ACC
BstPI 1748 GGTCACC G^GT[ACGT]ACC
BstXI 456 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 343 GCTGAGC GC^T[ACGT]AGC
CfoI 473 GCGC GCG^C
DraII 552 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1455 GACATCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 552 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1748 GGTCACC G^GT[ACGT]ACC
EcoRI 2304 GAATTC G^AATTC
FauI 1970 CCCGC CCCGC(4/6)
HaeII 472 GGCGCC [GA]GCGC^[CT]
HapII 1027 CCGG C^CGG
HgaI 489 GACGC GACGC(5/10)
HhaI 473 GCGC GCG^C
Hin1I 472 GGCGCC G[GA]^CG[CT]C
HinP1I 473 GCGC G^CGC
HindIII 1672 AAGCTT A^AGCTT
HpaI 214 GTTAAC GTT^AAC
HpaII 1027 CCGG C^CGG
KasI 472 GGCGCC G^GCGCC
KpnI 1830 GGTACC GGTAC^C
MfeI 8 CAATTG C^AATTG
MluNI 93 TGGCCA TGG^CCA
MroI 1026 TCCGGA T^CCGGA
MscI 93 TGGCCA TGG^CCA
MspI 1027 CCGG C^CGG
MunI 8 CAATTG C^AATTG
NarI 472 GGCGCC GG^CGCC
PaeR7I 602 CTCGAG C^TCGAG
PflMI 1585 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 552 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2014 ATTAAT AT^TAAT
Psp1406I 974 AACGTT AA^CGTT
SacI 857 GAGCTC GAGCT^C
SalI 589 GTCGAC G^TCGAC
SfcI 891 CTATAG C^T[GA][CT]AG
SfoI 472 GGCGCC GGC^GCC
SpeI 2091 ACTAGT A^CTAGT
SspBI 1520 TGTACA T^GTACA
SspI 19 AATATT AAT^ATT
Van91I 1585 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1841 CCAGCATTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 602 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 1280 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1445 CAGTAGCTG
AspHI 432 GAGCAC G[AT]GC[AT]^C
857 GAGCTC
BanI 472 GGCGCC G^G[CT][GA]CC
1830 GGTACC
BfaI 485 CTAG C^TAG
2092 CTAG
BsiHKAI 432 GAGCAC G[AT]GC[AT]^C
857 GAGCTC
BsmAI 2127 GAGAC GTCTC(1/5)
411 GTCTC
BspHI 768 TCATGA T^CATGA
1071 TCATGA
BspMI 2061 ACCTGC ACCTGC(4/8)
934 GCAGGT
Bst1107I 618 GTATAC GTA^TAC
2369 GTATAC
BstZ17I 618 GTATAC GTA^TAC
2369 GTATAC
EaeI 93 TGGCCA [CT]^GGCC[GA]
1995 TGGCCG
EarI 667 GAAGAG CTCTTC(1/4)
718 GAAGAG
EcoT14I 1536 CCATGG C^C[AT][AT]GG
2185 CCATGG
Ksp632I 667 GAAGAG CTCTTC(1/4)
718 GAAGAG
MaeI 485 CTAG C^TAG
2092 CTAG
MslI 1600 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1845 CATTTCTGTG
NcoI 1536 CCATGG C^CATGG
2185 CCATGG
RcaI 768 TCATGA T^CATGA
1071 TCATGA
SmlI 602 CTCGAG C^T[CT][GA]AG
2113 CTTAAG
StyI 1536 CCATGG C^C[AT][AT]GG
2185 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BciVI
BmyI BpmI BsaBI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BstF5I BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EcoT22I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MspA1I MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PvuII RsaI
Sau3AI Sau96I SfaNI SphI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII