##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BamHI BciVI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI KpnI
KspI MamI MluI MroI MvnI
NdeI NheI NotI NruI NspV
PaeR7I PflMI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2228 GACGTC GACGT^C
AccI 5554 GTAGAC GT^[AC][GT]AC
AflII 988 CTTAAG C^TTAAG
AocI 2165 CCTCAGG CC^T[ACGT]AGG
AseI 2461 ATTAAT AT^TAAT
AsnI 2461 ATTAAT AT^TAAT
Asp700I 268 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 3334 GGCGCC GGCGC^C
BbrPI 4179 CACGTG CAC^GTG
BbvCI 2551 GCTGAGG CCTCAGC(-5/-2)
BclI 1733 TGATCA T^GATCA
BfrI 988 CTTAAG C^TTAAG
BsaWI 4205 ACCGGA [AT]^CCGG[AT]
BsmBI 588 GAGACG CGTCTC(1/5)
BsrFI 3753 GCCGGC [GA]^CCGG[CT]
Bsu36I 2165 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3753 GCCGGC [GA]^CCGG[CT]
Eco81I 2165 CCTCAGG CC^T[ACGT]AGG
FbaI 1733 TGATCA T^GATCA
HgaI 3906 GACGC GACGC(5/10)
HindIII 4123 AAGCTT A^AGCTT
KasI 3334 GGCGCC G^GCGCC
NaeI 3753 GCCGGC GCC^GGC
NarI 3334 GGCGCC GG^CGCC
NgoMIV 3753 GCCGGC G^CCGGC
PacI 1571 TTAATTAA TTAAT^TAA
PmaCI 4179 CACGTG CAC^GTG
PmlI 4179 CACGTG CAC^GTG
PshBI 2461 ATTAAT AT^TAAT
SfoI 3334 GGCGCC GGC^GCC
SpeI 4397 ACTAGT A^CTAGT
SspI 5037 AATATT AAT^ATT
SwaI 1190 ATTTAAAT ATTT^AAAT
XcmI 3957 CCAGTGTGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 268 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 1307 GTGCAC G^TGCAC
6144 GTGCAC
ApaLI 1307 GTGCAC G^TGCAC
6144 GTGCAC
AspI 3358 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6070 GACCCTGTC
BcgI 6136 CGAATCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3573 GCAGGTGAATCG
BspHI 5454 TCATGA T^CATGA
5903 TCATGA
BspLU11I 3449 ACATGT A^CATGT
4849 ACATGT
BstAPI 148 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4203 GCACCGGATGC
EcoNI 794 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5845 CCTTTGGTAGG
FauI 1400 CCCGC CCCGC(4/6)
2534 GCGGG
HaeII 1334 GGCGCT [GA]GCGC^[CT]
3334 GGCGCC
HpaI 1145 GTTAAC GTT^AAC
4845 GTTAAC
MfeI 1094 CAATTG C^AATTG
4827 CAATTG
MunI 1094 CAATTG C^AATTG
4827 CAATTG
PflFI 3358 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6070 GACCCTGTC
RcaI 5454 TCATGA T^CATGA
5903 TCATGA
SacI 1216 GAGCTC GAGCT^C
2239 GAGCTC
Tth111I 3358 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6070 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshAI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI