##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Alw44I ApaLI AscI Asp718I AviII
BbrPI BclI BsiEI BsiWI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoT22I FbaI
FseI FspI HindIII KpnI KspI
MluI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SnaBI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 1265 GGCGCC G[GA]^CG[CT]C
AflIII 4771 ACATGT A^C[GA][CT]GT
AhdI 2182 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 224 AGCGCT AGC^GCT
AspEI 2182 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5095 GGATCC G^GATCC
BbeI 1265 GGCGCC GGCGC^C
BcgI 3812 GCAGATTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4366 GTATCC GTATCC(6/5)
BsaHI 1265 GGCGCC G[GA]^CG[CT]C
BspHI 3215 TCATGA T^CATGA
BspLU11I 4771 ACATGT A^CATGT
BsrBI 3114 CCGCTC CCGCTC(-3/-3)
BstBI 3088 TTCGAA TT^CGAA
BstEII 5956 GGTCACC G^GT[ACGT]ACC
BstPI 5956 GGTCACC G^GT[ACGT]ACC
DraIII 1012 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1522 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2182 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 224 AGCGCT AGC^GCT
EcoO65I 5956 GGTCACC G^GT[ACGT]ACC
EcoRV 4710 GATATC GAT^ATC
Hin1I 1265 GGCGCC G[GA]^CG[CT]C
HpaI 4962 GTTAAC GTT^AAC
KasI 1265 GGCGCC G^GCGCC
MfeI 433 CAATTG C^AATTG
MunI 433 CAATTG C^AATTG
NaeI 2127 GCCGGC GCC^GGC
NarI 1265 GGCGCC GG^CGCC
NdeI 5472 CATATG CA^TATG
NgoMIV 2127 GCCGGC G^CCGGC
NspV 3088 TTCGAA TT^CGAA
RcaI 3215 TCATGA T^CATGA
ScaI 2751 AGTACT AGT^ACT
SexAI 1664 ACCTGGT A^CC[AT]GGT
SfoI 1265 GGCGCC GGC^GCC
SfuI 3088 TTCGAA TT^CGAA
SgrAI 1267 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 160 CCCGGG CCC^GGG
SpeI 2569 ACTAGT A^CTAGT
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 2347 CTTAAG C^TTAAG
2596 CTTAAG
ApaI 3144 GGGCCC GGGCC^C
5846 GGGCCC
AseI 4653 ATTAAT AT^TAAT
4877 ATTAAT
AsnI 4653 ATTAAT AT^TAAT
4877 ATTAAT
AvaI 160 CCCGGG C^[CT]CG[GA]G
327 CCCGAG
BanI 1265 GGCGCC G^G[CT][GA]CC
4924 GGCACC
BfrI 2347 CTTAAG C^TTAAG
2596 CTTAAG
BsaAI 2970 CACGTA [CT]AC^GT[GA]
3388 TACGTG
BsmBI 3078 CGTCTC CGTCTC(1/5)
6330 CGTCTC
BsoBI 160 CCCGGG C^[CT]CG[GA]G
327 CCCGAG
BssSI 1638 CACGAG CACGAG(-5/-1)
5465 CACGAG
HgaI 194 GACGC GACGC(5/10)
1610 GCGTC
PflMI 4079 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6453 CCACTCCATGG
PpuMI 2507 AGGACCC [GA]G^G[AT]CC[CT]
5250 AGGACCC
PshBI 4653 ATTAAT AT^TAAT
4877 ATTAAT
SacI 887 GAGCTC GAGCT^C
5336 GAGCTC
SbfI 1485 CCTGCAGG CCTGCA^GG
5641 CCTGCAGG
SfiI 1218 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1431 GGCCCTGCTGGCC
SphI 1142 GCATGC GCATG^C
3799 GCATGC
Sse8387I 1485 CCTGCAGG CCTGCA^GG
5641 CCTGCAGG
Van91I 4079 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6453 CCACTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaII AvrII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspEI BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI