##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AscI        Asp718I     AvrII       BbeI        BbvCI       
BcgI        BfrI        BlnI        BsiWI       BspDI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco52I      FseI        KasI        
KpnI        KspI        MluI        NaeI        NarI        
NgoMIV      NheI        NotI        NspV        PacI        
PaeR7I      PinAI       PmeI        Psp1406I    PvuI        
RsrII       SacII       SalI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AhdI         1008  GACTCACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3191  AGCGCT               AGC^GCT
AseI         5847  ATTAAT               AT^TAAT
AsnI         5847  ATTAAT               AT^TAAT
AspEI        1008  GACTCACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1920  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         841  TGCGCA               TGC^GCA
BanI         2934  GGCACC               G^G[CT][GA]CC
BbrPI        4928  CACGTG               CAC^GTG
BglI         2949  GCCGACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        4915  AGATCT               A^GATCT
BsaBI        3318  GATATCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1014  CGGTCG               CG[GA][CT]^CG
BsrBI        4733  GAGCGG               CCGCTC(-3/-3)
BsrFI        4548  ACCGGC               [GA]^CCGG[CT]
BssHII        734  GCGCGC               G^CGCGC
Cfr10I       4548  ACCGGC               [GA]^CCGG[CT]
DraIII        739  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1771  GACAGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1008  GACTCACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3191  AGCGCT               AGC^GCT
EcoRV        3318  GATATC               GAT^ATC
FspI          841  TGCGCA               TGC^GCA
HpaI         5813  GTTAAC               GTT^AAC
MamI         3318  GATATCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1049  CAATTG               C^AATTG
MunI         1049  CAATTG               C^AATTG
NdeI         3743  CATATG               CA^TATG
NruI         4406  TCGCGA               TCG^CGA
PflFI        1920  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        4928  CACGTG               CAC^GTG
PmlI         4928  CACGTG               CAC^GTG
PshAI        1047  GACAATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        5847  ATTAAT               AT^TAAT
SacI         1243  GAGCTC               GAGCT^C
SbfI         5192  CCTGCAGG             CCTGCA^GG
SmaI         2418  CCCGGG               CCC^GGG
SmlI         1795  CTCAAG               C^T[CT][GA]AG
SpeI         1498  ACTAGT               A^CTAGT
SphI         3616  GCATGC               GCATG^C
Sse8387I     5192  CCTGCAGG             CCTGCA^GG
SspI         5571  AATATT               AAT^ATT
Tth111I      1920  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         6231  TCTAGA               T^CTAGA
XmaCI        2418  CCCGGG               C^CCGGG
XmaI         2418  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI         2028  GACGCC               G[GA]^CG[CT]C
             3539  GGCGTC              
Alw44I       1257  GTGCAC               G^TGCAC
             5769  GTGCAC              
ApaI          341  GGGCCC               GGGCC^C
              519  GGGCCC              
ApaLI        1257  GTGCAC               G^TGCAC
             5769  GTGCAC              
BamHI         905  GGATCC               G^GATCC
             2442  GGATCC              
BciVI        1401  GGATAC               GTATCC(6/5)
             3433  GGATAC              
BlpI          654  GCTTAGC              GC^T[ACGT]AGC
             2208  GCTGAGC             
Bpu1102I      654  GCTTAGC              GC^T[ACGT]AGC
             2208  GCTGAGC             
BsaAI        4928  CACGTG               [CT]AC^GT[GA]
             5085  TACGTG              
BsaHI        2028  GACGCC               G[GA]^CG[CT]C
             3539  GGCGTC              
BsaI         3046  GAGACC               GGTCTC(1/5)
             3105  GAGACC              
BsmI         2572  GCATTC               GAATGC(1/-1)
             5980  GCATTC              
BstAPI        378  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1635  GCAACTGCTGC         
BstEII        417  GGTTACC              G^GT[ACGT]ACC
             1332  GGTGACC             
BstPI         417  GGTTACC              G^GT[ACGT]ACC
             1332  GGTGACC             
CelII         654  GCTTAGC              GC^T[ACGT]AGC
             2208  GCTGAGC             
EcoO65I       417  GGTTACC              G^GT[ACGT]ACC
             1332  GGTGACC             
EcoRI        3750  GAATTC               G^AATTC
             4329  GAATTC              
Hin1I        2028  GACGCC               G[GA]^CG[CT]C
             3539  GGCGTC              
HindIII       863  AAGCTT               A^AGCTT
             6024  AAGCTT              
SapI         4635  GAAGAGC              GCTCTTC(1/4)
             4986  GAAGAGC             
SexAI         180  ACCAGGT              A^CC[AT]GGT
             3626  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AfaI        AflIII      AluI        AlwI        AlwNI       
AocI        ApoI        Asp700I     AspHI       AvaI        
AvaII       BalI        BanII       BbsI        BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI