################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AscI Asp718I AvrII BbeI BbvCI BcgI BfrI BlnI BsiWI BspDI Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I FseI KasI KpnI KspI MluI NaeI NarI NgoMIV NheI NotI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AhdI 1008 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3191 AGCGCT AGC^GCT AseI 5847 ATTAAT AT^TAAT AsnI 5847 ATTAAT AT^TAAT AspEI 1008 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1920 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 841 TGCGCA TGC^GCA BanI 2934 GGCACC G^G[CT][GA]CC BbrPI 4928 CACGTG CAC^GTG BglI 2949 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4915 AGATCT A^GATCT BsaBI 3318 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1014 CGGTCG CG[GA][CT]^CG BsrBI 4733 GAGCGG CCGCTC(-3/-3) BsrFI 4548 ACCGGC [GA]^CCGG[CT] BssHII 734 GCGCGC G^CGCGC Cfr10I 4548 ACCGGC [GA]^CCGG[CT] DraIII 739 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1771 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1008 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3191 AGCGCT AGC^GCT EcoRV 3318 GATATC GAT^ATC FspI 841 TGCGCA TGC^GCA HpaI 5813 GTTAAC GTT^AAC MamI 3318 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1049 CAATTG C^AATTG MunI 1049 CAATTG C^AATTG NdeI 3743 CATATG CA^TATG NruI 4406 TCGCGA TCG^CGA PflFI 1920 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4928 CACGTG CAC^GTG PmlI 4928 CACGTG CAC^GTG PshAI 1047 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5847 ATTAAT AT^TAAT SacI 1243 GAGCTC GAGCT^C SbfI 5192 CCTGCAGG CCTGCA^GG SmaI 2418 CCCGGG CCC^GGG SmlI 1795 CTCAAG C^T[CT][GA]AG SpeI 1498 ACTAGT A^CTAGT SphI 3616 GCATGC GCATG^C Sse8387I 5192 CCTGCAGG CCTGCA^GG SspI 5571 AATATT AAT^ATT Tth111I 1920 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 6231 TCTAGA T^CTAGA XmaCI 2418 CCCGGG C^CCGGG XmaI 2418 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 2028 GACGCC G[GA]^CG[CT]C 3539 GGCGTC Alw44I 1257 GTGCAC G^TGCAC 5769 GTGCAC ApaI 341 GGGCCC GGGCC^C 519 GGGCCC ApaLI 1257 GTGCAC G^TGCAC 5769 GTGCAC BamHI 905 GGATCC G^GATCC 2442 GGATCC BciVI 1401 GGATAC GTATCC(6/5) 3433 GGATAC BlpI 654 GCTTAGC GC^T[ACGT]AGC 2208 GCTGAGC Bpu1102I 654 GCTTAGC GC^T[ACGT]AGC 2208 GCTGAGC BsaAI 4928 CACGTG [CT]AC^GT[GA] 5085 TACGTG BsaHI 2028 GACGCC G[GA]^CG[CT]C 3539 GGCGTC BsaI 3046 GAGACC GGTCTC(1/5) 3105 GAGACC BsmI 2572 GCATTC GAATGC(1/-1) 5980 GCATTC BstAPI 378 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1635 GCAACTGCTGC BstEII 417 GGTTACC G^GT[ACGT]ACC 1332 GGTGACC BstPI 417 GGTTACC G^GT[ACGT]ACC 1332 GGTGACC CelII 654 GCTTAGC GC^T[ACGT]AGC 2208 GCTGAGC EcoO65I 417 GGTTACC G^GT[ACGT]ACC 1332 GGTGACC EcoRI 3750 GAATTC G^AATTC 4329 GAATTC Hin1I 2028 GACGCC G[GA]^CG[CT]C 3539 GGCGTC HindIII 863 AAGCTT A^AGCTT 6024 AAGCTT SapI 4635 GAAGAGC GCTCTTC(1/4) 4986 GAAGAGC SexAI 180 ACCAGGT A^CC[AT]GGT 3626 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI