##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI Asp718I AvrII BbeI BbvCI
BcgI BfrI BlnI BsiWI BspDI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco52I FseI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AhdI 1008 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3191 AGCGCT AGC^GCT
AseI 5847 ATTAAT AT^TAAT
AsnI 5847 ATTAAT AT^TAAT
AspEI 1008 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1920 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 841 TGCGCA TGC^GCA
BanI 2934 GGCACC G^G[CT][GA]CC
BbrPI 4928 CACGTG CAC^GTG
BglI 2949 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4915 AGATCT A^GATCT
BsaBI 3318 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1014 CGGTCG CG[GA][CT]^CG
BsrBI 4733 GAGCGG CCGCTC(-3/-3)
BsrFI 4548 ACCGGC [GA]^CCGG[CT]
BssHII 734 GCGCGC G^CGCGC
Cfr10I 4548 ACCGGC [GA]^CCGG[CT]
DraIII 739 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1771 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1008 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3191 AGCGCT AGC^GCT
EcoRV 3318 GATATC GAT^ATC
FspI 841 TGCGCA TGC^GCA
HpaI 5813 GTTAAC GTT^AAC
MamI 3318 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1049 CAATTG C^AATTG
MunI 1049 CAATTG C^AATTG
NdeI 3743 CATATG CA^TATG
NruI 4406 TCGCGA TCG^CGA
PflFI 1920 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4928 CACGTG CAC^GTG
PmlI 4928 CACGTG CAC^GTG
PshAI 1047 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5847 ATTAAT AT^TAAT
SacI 1243 GAGCTC GAGCT^C
SbfI 5192 CCTGCAGG CCTGCA^GG
SmaI 2418 CCCGGG CCC^GGG
SmlI 1795 CTCAAG C^T[CT][GA]AG
SpeI 1498 ACTAGT A^CTAGT
SphI 3616 GCATGC GCATG^C
Sse8387I 5192 CCTGCAGG CCTGCA^GG
SspI 5571 AATATT AAT^ATT
Tth111I 1920 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6231 TCTAGA T^CTAGA
XmaCI 2418 CCCGGG C^CCGGG
XmaI 2418 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 2028 GACGCC G[GA]^CG[CT]C
3539 GGCGTC
Alw44I 1257 GTGCAC G^TGCAC
5769 GTGCAC
ApaI 341 GGGCCC GGGCC^C
519 GGGCCC
ApaLI 1257 GTGCAC G^TGCAC
5769 GTGCAC
BamHI 905 GGATCC G^GATCC
2442 GGATCC
BciVI 1401 GGATAC GTATCC(6/5)
3433 GGATAC
BlpI 654 GCTTAGC GC^T[ACGT]AGC
2208 GCTGAGC
Bpu1102I 654 GCTTAGC GC^T[ACGT]AGC
2208 GCTGAGC
BsaAI 4928 CACGTG [CT]AC^GT[GA]
5085 TACGTG
BsaHI 2028 GACGCC G[GA]^CG[CT]C
3539 GGCGTC
BsaI 3046 GAGACC GGTCTC(1/5)
3105 GAGACC
BsmI 2572 GCATTC GAATGC(1/-1)
5980 GCATTC
BstAPI 378 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1635 GCAACTGCTGC
BstEII 417 GGTTACC G^GT[ACGT]ACC
1332 GGTGACC
BstPI 417 GGTTACC G^GT[ACGT]ACC
1332 GGTGACC
CelII 654 GCTTAGC GC^T[ACGT]AGC
2208 GCTGAGC
EcoO65I 417 GGTTACC G^GT[ACGT]ACC
1332 GGTGACC
EcoRI 3750 GAATTC G^AATTC
4329 GAATTC
Hin1I 2028 GACGCC G[GA]^CG[CT]C
3539 GGCGTC
HindIII 863 AAGCTT A^AGCTT
6024 AAGCTT
SapI 4635 GAAGAGC GCTCTTC(1/4)
4986 GAAGAGC
SexAI 180 ACCAGGT A^CC[AT]GGT
3626 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AvaI
AvaII BalI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI