##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
BamHI BbeI BcgI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII BssSI BstBI
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
HaeII HindIII HpaI KasI KpnI
MamI MluI MroI NarI NdeI
NheI NotI NruI NspV PacI
PshBI PvuI PvuII RsrII SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspBI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 6085 AACGTT AA^CGTT
AcyI 5354 GACGCC G[GA]^CG[CT]C
AflII 6290 CTTAAG C^TTAAG
AgeI 5580 ACCGGT A^CCGGT
AlwNI 5767 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 5485 CCTGAGG CC^T[ACGT]AGG
Asp700I 1883 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 7 TGCGCA TGC^GCA
BbrPI 4144 CACGTG CAC^GTG
BclI 5933 TGATCA T^GATCA
BfrI 6290 CTTAAG C^TTAAG
BglII 920 AGATCT A^GATCT
BlpI 97 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 97 GCTCAGC GC^T[ACGT]AGC
BsaHI 5354 GACGCC G[GA]^CG[CT]C
BsmI 1595 GCATTC GAATGC(1/-1)
BspLU11I 4860 ACATGT A^CATGT
BsrBI 95 CCGCTC CCGCTC(-3/-3)
Bst1107I 4877 GTATAC GTA^TAC
BstAPI 3470 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4877 GTATAC GTA^TAC
Bsu36I 5485 CCTGAGG CC^T[ACGT]AGG
CelII 97 GCTCAGC GC^T[ACGT]AGC
Eco81I 5485 CCTGAGG CC^T[ACGT]AGG
EcoNI 5646 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4186 GATATC GAT^ATC
EcoT22I 1593 ATGCAT ATGCA^T
FbaI 5933 TGATCA T^GATCA
FspI 7 TGCGCA TGC^GCA
Hin1I 5354 GACGCC G[GA]^CG[CT]C
NaeI 6278 GCCGGC GCC^GGC
NgoMIV 6278 GCCGGC G^CCGGC
NsiI 1593 ATGCAT ATGCA^T
PaeR7I 3591 CTCGAG C^TCGAG
PinAI 5580 ACCGGT A^CCGGT
PmaCI 4144 CACGTG CAC^GTG
PmeI 6463 GTTTAAAC GTTT^AAAC
PmlI 4144 CACGTG CAC^GTG
PshAI 5541 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6085 AACGTT AA^CGTT
SalI 5692 GTCGAC G^TCGAC
SexAI 2353 ACCAGGT A^CC[AT]GGT
SphI 2368 GCATGC GCATG^C
SspI 3313 AATATT AAT^ATT
StuI 6357 AGGCCT AGG^CCT
SwaI 6435 ATTTAAAT ATTT^AAAT
XhoI 3591 CTCGAG C^TCGAG
XmnI 1883 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 189 GTGCAC G^TGCAC
3064 GTGCAC
ApaLI 189 GTGCAC G^TGCAC
3064 GTGCAC
AspI 2623 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3123 GACAGGGTC
AvrII 4574 CCTAGG C^CTAGG
5396 CCTAGG
BciVI 4915 GTATCC GTATCC(6/5)
5300 GTATCC
BlnI 4574 CCTAGG C^CTAGG
5396 CCTAGG
BsaWI 2871 TCCGGT [AT]^CCGG[AT]
5580 ACCGGT
BspMI 180 ACCTGC ACCTGC(4/8)
125 GCAGGT
BstEII 5583 GGTAACC G^GT[ACGT]ACC
6330 GGTGACC
BstPI 5583 GGTAACC G^GT[ACGT]ACC
6330 GGTGACC
DraIII 2352 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6004 CACTACGTG
EarI 5030 CTCTTC CTCTTC(1/4)
5292 CTCTTC
EcoO65I 5583 GGTAACC G^GT[ACGT]ACC
6330 GGTGACC
HincII 3829 GTTGAC GT[CT]^[GA]AC
5692 GTCGAC
HindII 3829 GTTGAC GT[CT]^[GA]AC
5692 GTCGAC
Ksp632I 5030 CTCTTC CTCTTC(1/4)
5292 CTCTTC
KspI 3058 CCGCGG CCGC^GG
3632 CCGCGG
PflFI 2623 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3123 GACAGGGTC
SacII 3058 CCGCGG CCGC^GG
3632 CCGCGG
ScaI 1865 AGTACT AGT^ACT
2384 AGTACT
SpeI 1885 ACTAGT A^CTAGT
2650 ACTAGT
Tth111I 2623 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3123 GACAGGGTC
XbaI 519 TCTAGA T^CTAGA
2085 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI