################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AatII AccIII AgeI Alw44I ApaLI AscI AviII BamHI BclI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BstBI ClaI CpoI EcoRV FbaI FspI HpaI MamI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SalI SfuI SnaBI SpeI SphI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 3602 GGTACC G^GTACC AclI 992 AACGTT AA^CGTT AflII 1670 CTTAAG C^TTAAG AseI 1472 ATTAAT AT^TAAT AsnI 1472 ATTAAT AT^TAAT Asp700I 4039 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3602 GGTACC G^GTACC BcgI 2007 CGAGGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1650 GTATCC GTATCC(6/5) BfrI 1670 CTTAAG C^TTAAG BsaWI 135 TCCGGT [AT]^CCGG[AT] BsiEI 3167 CGGCCG CG[GA][CT]^CG BspHI 5308 TCATGA T^CATGA BsrDI 4972 GCAATG GCAATG(2/0) BssSI 567 CTCGTG CACGAG(-5/-1) Bst1107I 1498 GTATAC GTA^TAC BstZ17I 1498 GTATAC GTA^TAC DraI 1968 TTTAAA TTT^AAA DrdI 5434 GACAGTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3167 CGGCCG C^GGCCG EclXI 3167 CGGCCG C^GGCCG Eco52I 3167 CGGCCG C^GGCCG EcoRI 2220 GAATTC G^AATTC EcoT22I 1086 ATGCAT ATGCA^T FseI 161 GGCCGGCC GGCCGG^CC HindIII 5479 AAGCTT A^AGCTT KpnI 3602 GGTACC GGTAC^C NheI 4144 GCTAGC G^CTAGC NsiI 1086 ATGCAT ATGCA^T PshBI 1472 ATTAAT AT^TAAT Psp1406I 992 AACGTT AA^CGTT RcaI 5308 TCATGA T^CATGA SbfI 3926 CCTGCAGG CCTGCA^GG ScaI 5083 AGTACT AGT^ACT SgrAI 2520 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 3926 CCTGCAGG CCTGCA^GG SspI 1637 AATATT AAT^ATT XbaI 711 TCTAGA T^CTAGA XmnI 4039 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AhdI 4211 GACGTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5331 GACACTGTGTC AspEI 4211 GACGTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5331 GACACTGTGTC AspI 2818 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4252 GACAGGGTC AvrII 837 CCTAGG C^CTAGG 4373 CCTAGG BglII 383 AGATCT A^GATCT 2148 AGATCT BlnI 837 CCTAGG C^CTAGG 4373 CCTAGG BlpI 3734 GCTGAGC GC^T[ACGT]AGC 4200 GCTCAGC Bpu1102I 3734 GCTGAGC GC^T[ACGT]AGC 4200 GCTCAGC BsaI 1996 GAGACC GGTCTC(1/5) 3781 GGTCTC BsmBI 4982 CGTCTC CGTCTC(1/5) 4941 GAGACG BsmI 2294 GAATGC GAATGC(1/-1) 2506 GAATGC CelII 3734 GCTGAGC GC^T[ACGT]AGC 4200 GCTCAGC DraIII 740 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 6278 CACTCGGTG Eam1105I 4211 GACGTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5331 GACACTGTGTC HincII 349 GTCAAC GT[CT]^[GA]AC 908 GTTGAC HindII 349 GTCAAC GT[CT]^[GA]AC 908 GTTGAC NcoI 3620 CCATGG C^CATGG 6219 CCATGG PflFI 2818 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4252 GACAGGGTC PflMI 778 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4081 CCAGAAGATGG PpuMI 4076 AGGTCCC [GA]G^G[AT]CC[CT] 4314 GGGACCC SacI 1069 GAGCTC GAGCT^C 1219 GAGCTC Tth111I 2818 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4252 GACAGGGTC Van91I 778 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4081 CCAGAAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 148 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PmaCI PmlI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI