##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccIII AgeI Alw44I ApaLI
AscI AviII BamHI BclI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BstBI ClaI CpoI
EcoRV FbaI FspI HpaI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI RsrII
SalI SfuI SnaBI SpeI SphI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3602 GGTACC G^GTACC
AclI 992 AACGTT AA^CGTT
AflII 1670 CTTAAG C^TTAAG
AseI 1472 ATTAAT AT^TAAT
AsnI 1472 ATTAAT AT^TAAT
Asp700I 4039 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3602 GGTACC G^GTACC
BcgI 2007 CGAGGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1650 GTATCC GTATCC(6/5)
BfrI 1670 CTTAAG C^TTAAG
BsaWI 135 TCCGGT [AT]^CCGG[AT]
BsiEI 3167 CGGCCG CG[GA][CT]^CG
BspHI 5308 TCATGA T^CATGA
BsrDI 4972 GCAATG GCAATG(2/0)
BssSI 567 CTCGTG CACGAG(-5/-1)
Bst1107I 1498 GTATAC GTA^TAC
BstZ17I 1498 GTATAC GTA^TAC
DraI 1968 TTTAAA TTT^AAA
DrdI 5434 GACAGTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3167 CGGCCG C^GGCCG
EclXI 3167 CGGCCG C^GGCCG
Eco52I 3167 CGGCCG C^GGCCG
EcoRI 2220 GAATTC G^AATTC
EcoT22I 1086 ATGCAT ATGCA^T
FseI 161 GGCCGGCC GGCCGG^CC
HindIII 5479 AAGCTT A^AGCTT
KpnI 3602 GGTACC GGTAC^C
NheI 4144 GCTAGC G^CTAGC
NsiI 1086 ATGCAT ATGCA^T
PshBI 1472 ATTAAT AT^TAAT
Psp1406I 992 AACGTT AA^CGTT
RcaI 5308 TCATGA T^CATGA
SbfI 3926 CCTGCAGG CCTGCA^GG
ScaI 5083 AGTACT AGT^ACT
SgrAI 2520 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 3926 CCTGCAGG CCTGCA^GG
SspI 1637 AATATT AAT^ATT
XbaI 711 TCTAGA T^CTAGA
XmnI 4039 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 4211 GACGTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5331 GACACTGTGTC
AspEI 4211 GACGTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5331 GACACTGTGTC
AspI 2818 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4252 GACAGGGTC
AvrII 837 CCTAGG C^CTAGG
4373 CCTAGG
BglII 383 AGATCT A^GATCT
2148 AGATCT
BlnI 837 CCTAGG C^CTAGG
4373 CCTAGG
BlpI 3734 GCTGAGC GC^T[ACGT]AGC
4200 GCTCAGC
Bpu1102I 3734 GCTGAGC GC^T[ACGT]AGC
4200 GCTCAGC
BsaI 1996 GAGACC GGTCTC(1/5)
3781 GGTCTC
BsmBI 4982 CGTCTC CGTCTC(1/5)
4941 GAGACG
BsmI 2294 GAATGC GAATGC(1/-1)
2506 GAATGC
CelII 3734 GCTGAGC GC^T[ACGT]AGC
4200 GCTCAGC
DraIII 740 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
6278 CACTCGGTG
Eam1105I 4211 GACGTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5331 GACACTGTGTC
HincII 349 GTCAAC GT[CT]^[GA]AC
908 GTTGAC
HindII 349 GTCAAC GT[CT]^[GA]AC
908 GTTGAC
NcoI 3620 CCATGG C^CATGG
6219 CCATGG
PflFI 2818 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4252 GACAGGGTC
PflMI 778 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4081 CCAGAAGATGG
PpuMI 4076 AGGTCCC [GA]G^G[AT]CC[CT]
4314 GGGACCC
SacI 1069 GAGCTC GAGCT^C
1219 GAGCTC
Tth111I 2818 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4252 GACAGGGTC
Van91I 778 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4081 CCAGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
PvuII RsaI SacII SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI