##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I ApaLI AscI Asp718I
AspI AviII BbvCI BclI BfrI
BglI BseAI BsiEI BsiWI BsmBI
BspEI BssHII Bst1107I BstBI BstXI
BstZ17I CpoI DraII DraIII DsaI
EagI EclXI Eco52I EcoO109I FauI
FbaI FseI FspI HpaI KpnI
KspI MluI MroI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SbfI SexAI SfiI SfuI
SgrAI SmaI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1634 GTCGAC GT^[AC][GT]AC
AccII 35 CGCG CG^CG
Aor51HI 2143 AGCGCT AGC^GCT
ApaI 147 GGGCCC GGGCC^C
AvaI 296 CTCGAG C^[CT]CG[GA]G
AvrII 1750 CCTAGG C^CTAGG
BanII 147 GGGCCC G[GA]GC[CT]^C
BbeI 821 GGCGCC GGCGC^C
BbrPI 416 CACGTG CAC^GTG
BcgI 3858 CGAGGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1653 GTATCC GTATCC(6/5)
BcnI 1096 CCCGG CC^[GC]GG
BlnI 1750 CCTAGG C^CTAGG
BlpI 4804 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4804 GCTGAGC GC^T[ACGT]AGC
BsaWI 3321 ACCGGA [AT]^CCGG[AT]
BsgI 5105 CTGCAC GTGCAG(16/14)
BsoBI 296 CTCGAG C^[CT]CG[GA]G
BsrBI 1318 GAGCGG CCGCTC(-3/-3)
BsrFI 578 GCCGGT [GA]^CCGG[CT]
BstAPI 2443 GCAATTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3484 GGTGACC G^GT[ACGT]ACC
BstPI 3484 GGTGACC G^GT[ACGT]ACC
BstUI 35 CGCG CG^CG
CelII 4804 GCTGAGC GC^T[ACGT]AGC
Cfr10I 578 GCCGGT [GA]^CCGG[CT]
Eco47III 2143 AGCGCT AGC^GCT
EcoO65I 3484 GGTGACC G^GT[ACGT]ACC
EcoRV 3073 GATATC GAT^ATC
EcoT22I 1807 ATGCAT ATGCA^T
HgaI 6007 GCGTC GACGC(5/10)
KasI 821 GGCGCC G^GCGCC
MfeI 3593 CAATTG C^AATTG
MunI 3593 CAATTG C^AATTG
MvnI 35 CGCG CG^CG
NarI 821 GGCGCC GG^CGCC
NciI 1096 CCCGG CC^[GC]GG
NdeI 3490 CATATG CA^TATG
NsiI 1807 ATGCAT ATGCA^T
PaeR7I 296 CTCGAG C^TCGAG
PmaCI 416 CACGTG CAC^GTG
PmlI 416 CACGTG CAC^GTG
PstI 4331 CTGCAG CTGCA^G
SalI 1634 GTCGAC G^TCGAC
ScaI 3664 AGTACT AGT^ACT
SfoI 821 GGCGCC GGC^GCC
SnaBI 950 TACGTA TAC^GTA
SphI 909 GCATGC GCATG^C
XhoI 296 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 821 GGCGCC G[GA]^CG[CT]C
6006 GGCGTC
AspHI 326 GAGCAC G[AT]GC[AT]^C
413 GAGCAC
BalI 441 TGGCCA TGG^CCA
6152 TGGCCA
BamHI 43 GGATCC G^GATCC
1322 GGATCC
BanI 821 GGCGCC G^G[CT][GA]CC
5448 GGTGCC
BglII 5289 AGATCT A^GATCT
5784 AGATCT
BsaAI 416 CACGTG [CT]AC^GT[GA]
950 TACGTA
BsaHI 821 GGCGCC G[GA]^CG[CT]C
6006 GGCGTC
BsiHKAI 326 GAGCAC G[AT]GC[AT]^C
413 GAGCAC
Bsp1407I 3303 TGTACA T^GTACA
6320 TGTACA
BspDI 898 ATCGAT AT^CGAT
3296 ATCGAT
BspHI 540 TCATGA T^CATGA
4769 TCATGA
BspMI 3154 ACCTGC ACCTGC(4/8)
3499 ACCTGC
BsrGI 3303 TGTACA T^GTACA
6320 TGTACA
BssSI 323 CACGAG CACGAG(-5/-1)
5039 CTCGTG
ClaI 898 ATCGAT AT^CGAT
3296 ATCGAT
DrdI 1637 GACAGTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2559 GACTTTAGAGTC
EaeI 441 TGGCCA [CT]^GGCC[GA]
6152 TGGCCA
EcoNI 3010 CCTAATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5295 CCTCAAACAGG
Hin1I 821 GGCGCC G[GA]^CG[CT]C
6006 GGCGTC
MluNI 441 TGGCCA TGG^CCA
6152 TGGCCA
MscI 441 TGGCCA TGG^CCA
6152 TGGCCA
MslI 1465 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1547 CACATTTGTG
PflMI 2323 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3055 CCATTTGGTGG
RcaI 540 TCATGA T^CATGA
4769 TCATGA
SpeI 4399 ACTAGT A^CTAGT
4408 ACTAGT
SspBI 3303 TGTACA T^GTACA
6320 TGTACA
Van91I 2323 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3055 CCATTTGGTGG
XcmI 521 CCAAAGAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4607 CCAGGTCAGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AhdI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspEI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
Eam1105I EarI Eco81I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI