##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AgeI AhdI Aor51HI ApaI
AscI AspEI BamHI BbeI BcgI
BglI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FseI HaeII KasI KspI
MluI NarI NheI NotI NruI
PinAI PmeI PvuI RsrII SacII
SalI SapI SfiI SfoI SgrAI
SmaI SnaBI SphI SspBI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1386 GGTACC G^GTACC
AccIII 1463 TCCGGA T^CCGGA
AclI 6408 AACGTT AA^CGTT
AcyI 3215 GACGCC G[GA]^CG[CT]C
Alw44I 5075 GTGCAC G^TGCAC
ApaLI 5075 GTGCAC G^TGCAC
Asp718I 1386 GGTACC G^GTACC
AviII 2404 TGCGCA TGC^GCA
BalI 3288 TGGCCA TGG^CCA
BanI 1386 GGTACC G^G[CT][GA]CC
BclI 5965 TGATCA T^GATCA
BlpI 2386 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2386 GCTGAGC GC^T[ACGT]AGC
BsaHI 3215 GACGCC G[GA]^CG[CT]C
BseAI 1463 TCCGGA T^CCGGA
BsiEI 2716 CGACCG CG[GA][CT]^CG
BspEI 1463 TCCGGA T^CCGGA
BsrBI 2602 CCGCTC CCGCTC(-3/-3)
BsrFI 2819 GCCGGC [GA]^CCGG[CT]
BssHII 2428 GCGCGC G^CGCGC
BstBI 6003 TTCGAA TT^CGAA
CelII 2386 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2819 GCCGGC [GA]^CCGG[CT]
DsaI 1782 CCATGG C^C[GA][CT]GG
EcoRV 5450 GATATC GAT^ATC
FbaI 5965 TGATCA T^GATCA
FspI 2404 TGCGCA TGC^GCA
Hin1I 3215 GACGCC G[GA]^CG[CT]C
HincII 1933 GTTAAC GT[CT]^[GA]AC
HindII 1933 GTTAAC GT[CT]^[GA]AC
HpaI 1933 GTTAAC GTT^AAC
KpnI 1386 GGTACC GGTAC^C
MfeI 4558 CAATTG C^AATTG
MluNI 3288 TGGCCA TGG^CCA
MroI 1463 TCCGGA T^CCGGA
MscI 3288 TGGCCA TGG^CCA
MunI 4558 CAATTG C^AATTG
NaeI 2819 GCCGGC GCC^GGC
NcoI 1782 CCATGG C^CATGG
NdeI 6539 CATATG CA^TATG
NgoMIV 2819 GCCGGC G^CCGGC
NspV 6003 TTCGAA TT^CGAA
PacI 4086 TTAATTAA TTAAT^TAA
PaeR7I 1008 CTCGAG C^TCGAG
Psp1406I 6408 AACGTT AA^CGTT
SacI 5410 GAGCTC GAGCT^C
SbfI 2281 CCTGCAGG CCTGCA^GG
SfuI 6003 TTCGAA TT^CGAA
SpeI 6440 ACTAGT A^CTAGT
Sse8387I 2281 CCTGCAGG CCTGCA^GG
StuI 2540 AGGCCT AGG^CCT
XbaI 4539 TCTAGA T^CTAGA
XhoI 1008 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2429 CGCG CG^CG
2752 CGCG
Asp700I 205 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6406 GAAACGTTTC
AspI 1264 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4125 GACAGAGTC
AvrII 3957 CCTAGG C^CTAGG
5748 CCTAGG
BlnI 3957 CCTAGG C^CTAGG
5748 CCTAGG
BspHI 3554 TCATGA T^CATGA
4170 TCATGA
BstUI 2429 CGCG CG^CG
2752 CGCG
DraII 731 GGGTCCT [GA]G^G[ACGT]CC[CT]
2614 GGGTCCC
DraIII 2107 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
6166 CACTGAGTG
EaeI 2765 CGGCCA [CT]^GGCC[GA]
3288 TGGCCA
EcoO109I 731 GGGTCCT [GA]G^G[ACGT]CC[CT]
2614 GGGTCCC
EcoRI 6149 GAATTC G^AATTC
6650 GAATTC
HgaI 3215 GACGC GACGC(5/10)
2422 GCGTC
MvnI 2429 CGCG CG^CG
2752 CGCG
PflFI 1264 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4125 GACAGAGTC
PpuMI 731 GGGTCCT [GA]G^G[AT]CC[CT]
2614 GGGTCCC
PshAI 1635 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3367 GACTGTGGTC
RcaI 3554 TCATGA T^CATGA
4170 TCATGA
ScaI 1501 AGTACT AGT^ACT
1905 AGTACT
SexAI 552 ACCAGGT A^CC[AT]GGT
5315 ACCAGGT
SwaI 6120 ATTTAAAT ATTT^AAAT
6500 ATTTAAAT
Tth111I 1264 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4125 GACAGAGTC
XcmI 234 CCAACATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1841 CCACTGCAAAGCTGG
XmnI 205 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6406 GAAACGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI AseI AsnI AspHI AvaI
AvaII BanII BbrPI BbsI BbvCI
BbvI BciVI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaAI
BsaBI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI Eco81I EcoO65I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PmaCI PmlI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII