##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AseI AsnI Asp718I AviII
BbrPI BciVI BfrI BglII BsaAI
BsaBI BsiEI BsiWI Bsp1407I BspDI
BsrGI BssSI BstBI ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoRV FseI FspI HpaI KpnI
MamI MluI NheI NotI NruI
NspV PacI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 3055 TCCGGA T^CCGGA
AscI 49 GGCGCGCC GG^CGCGCC
AspI 5311 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3883 CCTAGG C^CTAGG
BcgI 3526 GCACACTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3883 CCTAGG C^CTAGG
BseAI 3055 TCCGGA T^CCGGA
BspEI 3055 TCCGGA T^CCGGA
BspHI 3400 TCATGA T^CATGA
BssHII 50 GCGCGC G^CGCGC
Bst1107I 1908 GTATAC GTA^TAC
BstEII 1746 GGTGACC G^GT[ACGT]ACC
BstPI 1746 GGTGACC G^GT[ACGT]ACC
BstZ17I 1908 GTATAC GTA^TAC
DraI 5418 TTTAAA TTT^AAA
DrdI 884 GACTGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1746 GGTGACC G^GT[ACGT]ACC
EcoT22I 4434 ATGCAT ATGCA^T
HindIII 2206 AAGCTT A^AGCTT
MfeI 487 CAATTG C^AATTG
MroI 3055 TCCGGA T^CCGGA
MunI 487 CAATTG C^AATTG
NcoI 2302 CCATGG C^CATGG
NdeI 1162 CATATG CA^TATG
NsiI 4434 ATGCAT ATGCA^T
PflFI 5311 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 775 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3400 TCATGA T^CATGA
ScaI 3520 AGTACT AGT^ACT
SphI 3715 GCATGC GCATG^C
Tth111I 5311 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AatII 775 GACGTC GACGT^C
2724 GACGTC
AccI 1908 GTATAC GT^[AC][GT]AC
4390 GTAGAC
AgeI 1743 ACCGGT A^CCGGT
4133 ACCGGT
AhdI 2649 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACAGCTCGTC
Asp700I 2921 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3422 GAATAACTTC
AspEI 2649 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACAGCTCGTC
BamHI 3900 GGATCC G^GATCC
4404 GGATCC
BclI 4674 TGATCA T^GATCA
5400 TGATCA
BfaI 1290 CTAG C^TAG
3884 CTAG
BspLU11I 3316 ACATGT A^CATGT
6560 ACATGT
Eam1105I 2649 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACAGCTCGTC
FbaI 4674 TGATCA T^GATCA
5400 TGATCA
MaeI 1290 CTAG C^TAG
3884 CTAG
NaeI 1 GCCGGC GCC^GGC
2128 GCCGGC
NgoMIV 1 GCCGGC G^CCGGC
2128 GCCGGC
PinAI 1743 ACCGGT A^CCGGT
4133 ACCGGT
XmnI 2921 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3422 GAATAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmaI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI